Chemical Structure and Molecular Weights of α-(1→3)-<scp>D</scp>-Glucan from<i>Lentinus edodes</i>

Zhang, Pingyi; Zhang, Lina; Cheng, Shanshan

doi:10.1271/bbb.63.1197

Cited by 66 publications

(38 citation statements)

References 27 publications

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“…3)--D-glucan from different Lentinus edodes. 10) Moreover, the solution properties of O-sulfonated derivatives of (1 ! 3)--Dglucan from Lentinus edodes have been studied in our laboratory, indicating stiffer chains than the native glucan.…”

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confidence: 99%

Antitumor Activities ofO-Sulfonated Derivatives of (1→3)-α-D-Glucan from DifferentLentinus edodes

Surenjav

Zeng

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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confidence: 99%

Antitumor Activities ofO-Sulfonated Derivatives of (1→3)-α-D-Glucan from DifferentLentinus edodes

Surenjav

Zeng

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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“…0.05% NaBH4 two times, and precipitation with 36% acetic acid to remove L-FV-II, a-(1---+3)-D-glucan, according to previously reported method. 23 The supernatant was subjected to the Sevag method to remove proteins, and treated with 30% H20 2 to decolorize. Aqueous solution of the glucan was dialyzed against distilled water for 4 days, and concentrated by rotary evaporator at reduced pressure below 45°C, and lyophilized to obtain colorless flakes.…”

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Triple Helix of β-D-Glucan from Lentinus Edodes in 0.5 M NaCl Aqueous Solution Characterized by Light Scattering

Zhang

Zhou

et al. 2001

Polym J

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ABSTRACT:,B-(1-3)-D-glucan with (1-6) branching (L-FV-I) from Lentinus edodes in water was degraded into seven fractions of different molecular weights by ultrasonic irradiation. Weight-average molecular weight M w, radius of gyration , 112 and intrinsic viscosity [7]] of the ,B-o-glucan and its fractions in 0.5 M NaCl aqueous solution and dimethylsulfoxide (DMSO) were studied by multi-angle laser light scattering (MALLS), GPC combined with MALLS, and viscometry. M w dependence of [7]] for the glucan in 0.5 M NaCl aqueous solution was represented approximately by [7]] =7.69X 10-6 M / 32 (cm 3 g-1 ) at M w from 1.87 X 10 5 to 1.20 X 10 6 at 25°C. GPC chromatograms of the glucans in aqueous solution contained two peaks, a main peak corresponding to triple-stranded chains with molecular weight Mw,m, and small second peak corresponding to fragments of single chains with Mw, (about 20±5% content). Analysis of Mw m and ,m 112 in term of the known theory for wormlike chains yielded 2180± 100 nm -1, 120± 10 nm and 0.31 nm for.molar mass per unit contour length ML, persistence length q, and contour length h per main-chain glucose residue, respectively, which agree closely with theory data of triple-helical chains and reported parameters for triple-helix schizophyllan in 0.01 M NaOH aqueous solution. The ratios of Mw min 0.5 M NaCl to M win DMSO were calculated to be roughly 3. The predominant species of the glucan in 0.5 M NaCl ~queous solution exist as triple-helical chains with high rigidity, and in DMSO as single-flexible chains.

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“…Especially, the peak at 822.49 cm −1 is exclusively associated with (1→3)-α-linkages [14]. The lack of absorbance peaks at 890 cm −1 suggested that WIP contained only α-linked glucose [15].…”

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Fruiting bodies of Hericium erinaceus (Bull.) Pers. – a new source of water-insoluble (1→3)-α-d-glucan
Wiater
¹
,
Choma
²
,
Komaniecka
³

et al. 2016
Acta Soc Bot Pol
3
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A water-insoluble polysaccharide (WIP) was isolated from the fruiting bodies of Hericium erinaceus HE01 by an alkaline solution with the yield of 5%. Structural and compositional analyses by total acid hydrolysis, methylation analysis, FT-IR, FT-Raman, and 1 H NMR spectroscopy as well as other instrumental techniques showed predominantly glucose linked by α-glycosidic bonds and small amounts of mannose, xylose, rhamnose, galactose, and ribose. The methylation analysis showed that (1→3)-linked Glcp is the major constituent (70.8%) of the polymer, while the 3,4 substituted d-Glcp represents the main branching residue of the glucan. The presence of (1→3)-α-d-glucan in the hyphae of H. erinaceus was additionally confirmed by the use of specific fluorophore-labeled antibodies.

~~show abstract~~

Chemical Structure and Molecular Weights of α-(1→3)-D-Glucan fromLentinus edodes

Cited by 66 publications

References 27 publications

Antitumor Activities ofO-Sulfonated Derivatives of (1→3)-α-D-Glucan from DifferentLentinus edodes

Antitumor Activities ofO-Sulfonated Derivatives of (1→3)-α-D-Glucan from DifferentLentinus edodes

Triple Helix of β-D-Glucan from Lentinus Edodes in 0.5 M NaCl Aqueous Solution Characterized by Light Scattering

Fruiting bodies of Hericium erinaceus (Bull.) Pers. – a new source of water-insoluble (1→3)-α-d-glucan

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