“…e monosaccharide composition of DTMP was determined by gas chromatography-mass spectrometry (GC-MS) of Zhang et al [18], using an 7890A-5975GC instrument (Agilent Technologies, CA, USA). e sample (5 mg) was weighed and placed in a plug tube and hydrolyzed with 2 m L2.0 mol/L-1TFA (trifluoroacetic acid) at 110 °C for 3 h. After hydrolysis, hydroxylamine hydrochloride (10 mg), internal standard inositol hexaacetate (2 mg), and pyridine (0.5 ml) were added to the fully hydrolyzed sample.…”
The polysaccharide was extracted by water extraction and alcohol precipitation method from “deer tripe mushroom,” which was domesticated and bred from wild Auricularia delicata in Zambia. The structure of the deer tripe mushroom polysaccharide (DTMP) was characterized by gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR), scanning electron microscope (SEM), and other analytical techniques. And the rheological and gel properties and antioxidant capacities of the polysaccharide were studied. The results showed that DTMP was mainly composed of mannose, fructose, glucose, and galacturonic acid, of which the galacturonic acid was the main component. By calculation, the molar ratio of mannose : fructose : glucose : galactoacid in DTMP is 0.8 : 14.8 : 1.0 : 26.32. The polysaccharide was mainly composed by α-1,4-glycosidic bond. DTMP solution showed a shear-thinning (pseudoplastic) behaviour, and its pseudoplasticity was more obvious at a concentration of 2%. The power law model was used to evaluate the viscosity curves of DTMP, and its viscosity and consistency indices both increased as the concentration increased, whereas both indices decreased as the concentration decreased. The viscosity of the polysaccharide solution changed as the pH changed: the polysaccharide solutions had a higher viscosity at pH = 10. DTMP showed gel-like behaviour (G′ > G″), and the gel strength enhanced with the increase of concentration (2%–10%). In addition, the antioxidant experiment of DTMP showed that it had good antioxidant activity, and there was a significant dose-effect relationship between its activity and concentration in the low concentration range.
“…e monosaccharide composition of DTMP was determined by gas chromatography-mass spectrometry (GC-MS) of Zhang et al [18], using an 7890A-5975GC instrument (Agilent Technologies, CA, USA). e sample (5 mg) was weighed and placed in a plug tube and hydrolyzed with 2 m L2.0 mol/L-1TFA (trifluoroacetic acid) at 110 °C for 3 h. After hydrolysis, hydroxylamine hydrochloride (10 mg), internal standard inositol hexaacetate (2 mg), and pyridine (0.5 ml) were added to the fully hydrolyzed sample.…”
The polysaccharide was extracted by water extraction and alcohol precipitation method from “deer tripe mushroom,” which was domesticated and bred from wild Auricularia delicata in Zambia. The structure of the deer tripe mushroom polysaccharide (DTMP) was characterized by gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR), scanning electron microscope (SEM), and other analytical techniques. And the rheological and gel properties and antioxidant capacities of the polysaccharide were studied. The results showed that DTMP was mainly composed of mannose, fructose, glucose, and galacturonic acid, of which the galacturonic acid was the main component. By calculation, the molar ratio of mannose : fructose : glucose : galactoacid in DTMP is 0.8 : 14.8 : 1.0 : 26.32. The polysaccharide was mainly composed by α-1,4-glycosidic bond. DTMP solution showed a shear-thinning (pseudoplastic) behaviour, and its pseudoplasticity was more obvious at a concentration of 2%. The power law model was used to evaluate the viscosity curves of DTMP, and its viscosity and consistency indices both increased as the concentration increased, whereas both indices decreased as the concentration decreased. The viscosity of the polysaccharide solution changed as the pH changed: the polysaccharide solutions had a higher viscosity at pH = 10. DTMP showed gel-like behaviour (G′ > G″), and the gel strength enhanced with the increase of concentration (2%–10%). In addition, the antioxidant experiment of DTMP showed that it had good antioxidant activity, and there was a significant dose-effect relationship between its activity and concentration in the low concentration range.
“…Then 8 mL periodate oxidation solution and two drops of ethylene glycol was added to the original mixture to terminate the reaction. The yield of formed formic acid was titrated with 40% NaOH after adding two drops of phenolphthalein (27).…”
Section: Periodate Oxidation Smith Degradation and Methylation Analysismentioning
β-glucan has attracted extensive attention due to its health promoting effects, such as lowering the blood sugar and lipids levels, and enhancing immunity. In this study, three different β-glucans (HEBG-1, HEBG-2, HEBG-3) were obtained from Hericium erinaceus by sodium hydroxide, β-1,3-glucanase and β-1,6-glucanase, respectively. The effects of the glucans on in vitro digestion of wheat starch were investigated by Englyst method. We found that addition of HEBGs significantly reduced the digestibility of starch, showing as decreased RDS and pGI, and increased SDS and RS content. In addition, the inhibitory effects positively correlated with the molecular weight of HEBG. The triple helix structure in HEBG plays important roles in inhibiting starch digestion. And β-1,3- glucan showed stronger inhibitor effects than those of β-1,6- glucan. This study unravels the mechanism of HEBG on inhibition of starch digestion and provides a theoretical understanding for the application of edible mushroom β-glucan to the development of low glycemic index starchy foods.
“…4C and D), indicating that the polysaccharide chain structure of LVP partly consisted of (1/4)-linked and (1/4,6)-linked glycosidic bonds. 21 Periodate oxidation and Smith degradation can predict glycosidic linkages of the polysaccharide. At the same time, methylation analysis and NMR spectroscopy were performed to further determine the glycosidic bonds of monosaccharides in LVP.…”
“…The molar ratio of 2,3,6-Me 3 -Manp (1,4-linked Man, Me, methyl; Man, mannose) was higher than that of 2,3-Me 2 -Manp (1,4,6linked Man), revealed that the main chain included the /4) Manp (1/ residues. The degree of branch (DB) was calculated as follows: 21 DB ¼ (NT + NB)/(NT + NB + NL), where NT, NB, and NL are the numbers of terminal, branch, and linear residues, respectively. The calculated DB of LVP was 0.571 on basis of methylation data, showing its highly branched characteristic.…”
Section: Methylation and Nmr Analysis Of Lvpmentioning
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