Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour

Li, Xiao-Jun; Bao, Wan-Rong; Leung, Chung‐Hang; Ma, Dik‐Lung; Zhang, Ge; Lu, Aiping; Wang, Shunchun; Han, Quan‐Bin

doi:10.3390/molecules21060779

Cited by 27 publications

(5 citation statements)

References 40 publications

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“…Li et al isolated another neutral α-glucan, named BP1 19 (Mol. weight: 9.45 kDa), from Lobelia chinensis by hot-water extraction and studied its immunomodulating activities ( Li et al, 2016 ). The authors proposed that α-glucan BP1 activates Toll-like receptor 4 (TLR4) and exerts immunomodulating effects such as enhancement of the cell proliferation, nitric oxide production, cytokine secretion and phagocytosis in a dose-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

Beyond Alkaloids: Novel Bioactive Natural Products From Lobelia Species

2021

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In this work, we reviewed the progress in the phytochemical and biological investigations of bioactive components derived from medicinally valuable Lobelia species. In the last 60 years, Lobelia has garnered significant attention from the phytochemist from around the world, majorly due to the discovery of bioactive piperidine alkaloids (e.g., lobinaline and lobeline) in the early 1950s. Later, lobeline underwent clinical trials for several indications including the treatment of attention deficit hyperactivity disorder and a multicenter phase three trial for smoking cessation. Subsequently, several other alkaloids derived from different species of Lobelia were also investigated for their pharmacological characteristics. However, in the last few years, the research focus has started shifting to the characterization of the other novel chemical classes. The major shift has been noticed due to the structurally similar alkaloid components, which essentially share similar pharmacological, physicochemical, and toxicological profiles. In this review, we present an up-to-date overview of their progress with special attention to understanding the molecular mechanisms of the novel bioactive components.

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Section: Introductionmentioning

confidence: 99%

Beyond Alkaloids: Novel Bioactive Natural Products From Lobelia Species

2021

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“…Monosaccharide standards ( d -galactose, d -arabinose, l -rhamnose, l -fucose, d -mannose, d -xylose, and d -glucose) were treated as described previously. 52 , 53 Two milligrams of extracted mannans or monosaccharide standards were hydrolyzed with 2 M trifluoroacetic acid at 121 °C for 2 h. After evaporation, the residue was dissolved in 200 μl of H 2 O and reduced with 1 ml of 0.5 mol/l NaBH 4 DMSO solution at 40 °C for 90 min. Then, glacial acetic acid was added dropwise to the reaction tubes.…”

Section: Methodsmentioning

confidence: 99%

Priming with FLO8-deficient Candida albicans induces Th1-biased protective immunity against lethal polymicrobial sepsis

Han

et al. 2020

Cell Mol Immunol

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The morphological switch between yeast and hyphae of Candida albicans is essential for its interaction with the host defense system. However, the lack of understanding of host–pathogen interactions during C. albicans infection greatly hampers the development of effective immunotherapies. Here, we found that priming with the C. albicans FLO8-deficient (flo8) mutant, locked in yeast form, protected mice from subsequent lethal C. albicans infection. Deficiency of Dectin-2, a fungus-derived α-mannan recognition receptor, completely blocked flo8 mutant-induced protection. Mechanistically, the flo8 mutant-induced Dectin-2/CARD9-mediated IL-10 production in DCs and macrophages to block thymus atrophy by inhibiting the C. albicans-induced apoptosis of thymic T cells, which facilitated the continuous output of naive T cells from the thymus to the spleen. Continuous recruitment of naive T cells to the spleen enhanced Th1-biased antifungal immune responses. Consequently, depletion of CD4+ T cells or blockade of IL-10 receptor function using specific antibodies in mice completely blocked the protective effects of flo8 mutant priming against C. albicans infection. Moreover, mannans exposed on the surface of the flo8 mutant were responsible for eliciting protective immunity by inhibiting the C. albicans-induced apoptosis of thymic T cells to sustain the number of naive T cells in the spleen. Importantly, priming with the flo8 mutant extensively protected mice from polymicrobial infection caused by cecal ligation and puncture (CLP) by enhancing Th1-biased immune responses. Together, our findings imply that targeting FLO8 in C. albicans elicits protective immune responses against polymicrobial infections and that mannans extracted from the flo8 mutant are potential immunotherapeutic candidate(s) for controlling infectious diseases.

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“…4), and the results are summarized in Table 2. Based on the chemical shifts, methylation analysis results and reported literature, 37,[40][41][42][43] residues D-H were designated as →4-β-Glcp-1→, α-T-Manp-1→, →2-β-Glcp-1→, →4α-Glcp-1→, and →2-α-Glcp-1→, respectively.…”

Section: Nmr Analysismentioning

confidence: 99%

Preparation, structural characterization and prebiotic potential of mulberry leaf oligosaccharides

et al. 2022

Food Funct.

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Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour

Cited by 27 publications

References 40 publications

Beyond Alkaloids: Novel Bioactive Natural Products From Lobelia Species

Beyond Alkaloids: Novel Bioactive Natural Products From Lobelia Species

Priming with FLO8-deficient Candida albicans induces Th1-biased protective immunity against lethal polymicrobial sepsis

Preparation, structural characterization and prebiotic potential of mulberry leaf oligosaccharides

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