Abstract:Significant opportunities exist in the use of seafood by-products to create new beneficial products. Moreover, cephalopod skin is a good source of bioactive compounds. The present study compares the chemical structure properties and antioxidant activity of pigments extracted from the skin of Octopus vulgaris (OVS) and Dosidicus gigas (DGS) with methanol-HCl (T1) and ethanol-HCl (T2). The solubility and spectroscopic analysis (UV-Vis and 1 H NMR) indicated that extracted pigments belonged to the ommochrome fami… Show more
“…They also differ from those of porphyrins since no Soret band at ≈ 400 nm is distinguishable. These pro les turn out to be comparable to those of ommochromes which present a large band from 400 to 600 nm and a smaller one around 310 or 380 nm depending on the pH and the pigment in question 19,22,30 .…”
Section: Resultsmentioning
confidence: 59%
“…In this study, we noted that at least two groups of acid-soluble pigments were involved in the purple colour of shells of the oyster C. gigas. Among possible groups proposed from the identi cation of genes associated with their biosynthesis 9 , while the presence of acid-soluble porphyrins represented here by uroporphyrin I or III is now well established 7,8 , the presence of carotenoids and eumelanin could be ruled-out, their solubility being not compatible 1,30,38,39 . Besides, after separations of porphyrins from the set of acid-soluble pigments, the resulting PF was slightly soluble at 1 mg/mL in methanol, turning fully soluble with the addition of HCl (aq) , a typical property of ommochrome pigments.…”
Section: Discussionmentioning
confidence: 99%
“…While ommatins have an absorption band with a λ max around 450-500 nm, ommin A absorbs around 520 nm. The wide absorption band of PF, from 400 to 600 nm with λ max at 464, 496 and 552 nm, can result from a combination of various ommochromes pigments 30 (ommatins and ommins, at least). The discrimination between pheomelanin and ommochromes was then conducted by alkaline oxidation of PF, where none of pheomelanin markers were identi ed.…”
Among the great variety of pigments found in living beings in general, and more particularly in molluscs, the ommochromes are a family of unknown organic dyes and until now still too little studied. Several lines of physicochemical and structural evidence allowed us here to complete the composition of the purple colour of shell patterns of Crassostrea gigas, highlighting an intriguing association of ommochromes and porphyrins, corroborated by the presence of known genes associated with their biosynthetic pathways. We describe here our pathway to demonstrate for the first time the presence of ommochromes in a bivalve.
“…They also differ from those of porphyrins since no Soret band at ≈ 400 nm is distinguishable. These pro les turn out to be comparable to those of ommochromes which present a large band from 400 to 600 nm and a smaller one around 310 or 380 nm depending on the pH and the pigment in question 19,22,30 .…”
Section: Resultsmentioning
confidence: 59%
“…In this study, we noted that at least two groups of acid-soluble pigments were involved in the purple colour of shells of the oyster C. gigas. Among possible groups proposed from the identi cation of genes associated with their biosynthesis 9 , while the presence of acid-soluble porphyrins represented here by uroporphyrin I or III is now well established 7,8 , the presence of carotenoids and eumelanin could be ruled-out, their solubility being not compatible 1,30,38,39 . Besides, after separations of porphyrins from the set of acid-soluble pigments, the resulting PF was slightly soluble at 1 mg/mL in methanol, turning fully soluble with the addition of HCl (aq) , a typical property of ommochrome pigments.…”
Section: Discussionmentioning
confidence: 99%
“…While ommatins have an absorption band with a λ max around 450-500 nm, ommin A absorbs around 520 nm. The wide absorption band of PF, from 400 to 600 nm with λ max at 464, 496 and 552 nm, can result from a combination of various ommochromes pigments 30 (ommatins and ommins, at least). The discrimination between pheomelanin and ommochromes was then conducted by alkaline oxidation of PF, where none of pheomelanin markers were identi ed.…”
Among the great variety of pigments found in living beings in general, and more particularly in molluscs, the ommochromes are a family of unknown organic dyes and until now still too little studied. Several lines of physicochemical and structural evidence allowed us here to complete the composition of the purple colour of shell patterns of Crassostrea gigas, highlighting an intriguing association of ommochromes and porphyrins, corroborated by the presence of known genes associated with their biosynthetic pathways. We describe here our pathway to demonstrate for the first time the presence of ommochromes in a bivalve.
“…The head skin, considered a by-product, was manually collected, freeze-dried (Labconco, Kansas City, MO, USA), and kept at −18 ± 2 °C. Pigment extracts were prepared from 100 mg of freeze-dried skin samples via extraction with 1% HCl-methanol solution (v/v), as described elsewhere (Esparza-Espinoza et al, 2021). The samples without solvent were freeze-dried, packaged in polyethene bags, and stored at −18 ± 2 °C.…”
Section: Preparation Of Cephalopod Skin Pigment Extractsmentioning
confidence: 99%
“…This behavior could be due to the presence of antioxidant compounds in the pigments, which may exert a certain level of lipid oxidation inhibition. This capacity is based on the configuration of ommochromic compounds present in the pigment extracts (Esparza-Espinoza et al, 2021). Ommochromes can act as free radical acceptors, thereby decreasing oxidation and forming new radicals in the oxidation process (Romero & Martínez, 2015).…”
Bidens pilosa L. is a species of plant that grows wild. It is commonly found in abundance in the Mekong Delta region of Vietnam. This study aimed to investigate the chemical composition, in vitro and in vivo antioxidant activity, the antimicrobial activity against some aquatic pathogenic bacteria and antifungal activity against plant pathogenic bacteria of Bidens pilosa L. extract. The results showed that Bidens pilosa L. extract had good antioxidant capacity through all four test methods of DPPH, ABTS •+ , RP, and TAC with the EC 50 values of 455.78 ± 3.28 μg/mL, 148.68 ± 2.02 μg/mL, 462.09 ± 12.57 μg/mL and 139.14 ± 4.34 μg/mL, respectively. Fruit flies fed on a diet supplemented with 0.5 g/mL of Bidens pilosa L. extract had an average lifespan of 2.15 times and 1.54 times longer under oxidative stress using 20 mM Paraquat and H 2 O 2 10%, respectively. In addition, the total polyphenols and total flavonoids in the extract were also determined to be 107.49 ± 4.04 mg GAE/g and 165.63 ± 2.90 mg QE/g, respectively.Regarding the antimicrobial activity, the ethanol extract of Bidens pilosa L. showed stronger resistance to Gram (+) S. agalactiae than the tested Gram (-) bacteria, including A. hydrophila, E. ictaluri, and A. dhakensis. In addition, the ethanol extract from Bidens pilosa L. also showed the better ability to inhibit the growth of the fungus Colletotrichum sp. (MIC = 1250 µg/mL) than that of Fusarium oxysporum (MIC = 2500 µg/mL).
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