Chemical, structural properties, and osteoconductive effectiveness of bone block derived from porcine cancellous bone

Abstract

The purpose of this study is to determine the efficacy of bioactive calcium phosphate obtained from porcine cancellous bone for the treatment of bone defects and nonunion. Porcine cancellous bone blocks were heat treated at 1300 degrees C for 2 h. The chemical composition, calcium-to-phosphate ratio, and microstructure of the porcine bone blocks were examined. For in vivo implantation, bone defects were created on the anteromedial aspect of the proximal tibia in seven beagle dogs and the xenograft bone blocks were placed into these defects. Plain radiographs were taken at 2-week intervals for roentgenographic evaluation. At 12 weeks, the specimens were stained with hematoxylin and eosin (H&E). The composition and morphology of heat-treated porcine cancellous bone were found to be similar to heat-treated human cancellous bone. Radiographs showed union between the host bone/bone-block interfaces. At 12 weeks, uniform and substantial new bone formation was observed. It is concluded that heat-treated porcine cancellous bone demonstrated effective osteoconductivity. This high-temperature heat-treatment technique has several advantages, including decreased risk of disease transmission and immunoreactivity, while also offering excellent biocompatibility.

Preparation and characterization of fluorinated porcine hydroxyapatite

“…Both chemical and thermal approaches have been used to prepare biological HA derived from animal bones 14,24,30,31) . Thermal treatment has several advantages: it is the only means to eliminate organic matter 32) and it decreases the risk of disease transmission and immunoreactivity 30) .…”

“…Thermal treatment has several advantages: it is the only means to eliminate organic matter 32) and it decreases the risk of disease transmission and immunoreactivity 30) . For biological HA prepared using the thermal approach, its characteristics depend on heating conditions such as the temperature, gas atmosphere, and heating time.…”

“…Although crystallinity increased with rising temperature, a small amount of β-TCP was formed at 800°C by the partial decomposition of HA 32) when oxygen was absent during the heat treatment 30) . In the present study, raw porcine bone blocks were calcined at 800°C for 2 h to eliminate all organic matter.…”

Effect of Porcine Cancellous Bones on Regeneration in Rats with Calvarial Defect

“…동종골은 동종의 사체나 생존해 있는 다른 공여자에게서 얻는 것으로서 이식재를 동결시키거나 동결건조, 탈회동결건 조, 그리고 방사선 조사, 고압 멸균 등을 가한 형태로 항원성을 제거하여 면역반응을 최소화시켜 사용하게 된다 [11]. 주로 사 람에서 동종골을 채취하는 방법은 고관절대치술(total hip replacement)에서 나오는 대퇴골두와 사체에서 얻게 되나, 사체 에서 채취하는 경우 살아있는 환자에서 채취할 때보다 4배 정도의 세균 감염률이 높은 것으로 알려져 있다 [3].…”

Decellularized Bovine Bone Graft for Zygomatic Bone Reconstruction

“…Bovine cancellous bones structure and morphology are similar to human cancellous bone, and additionally it has osteoconductivity ability [23]. However, the major disadvantage of xenogenous bone grafts is that it has immune response that is also stronger than that of allografts.…”