Decellularized Bovine Bone Graft for Zygomatic Bone Reconstruction

Karalashvili, L; Chichua, N; Menabde, G; Atskvereli, L; Grdzelidze, T; Machavariani, A; Zurmukhtashvili, Marika; Gogilashvili, Ketevan; Kakabadze, Zurab; Z, Chichua

doi:10.21767/2471-8041.100087

Cited by 8 publications

(9 citation statements)

References 27 publications

(24 reference statements)

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“…In the other three patients under the same condition, the mandibular defect was repaired by autogenous rib graft. All four patients were examined twice a year after mandibular repair, none of whome had any particular problems [100]. In an experiment like this, decellularized xenograft-derived bovine bone scaffolds was used as a bone graft in a tibial defect in a 58-year-old woman.…”

Section: Infrequent Clinical Trial Of Bioscaffolds In the Human Bodymentioning

confidence: 99%

Application of decellularized bone matrix as a bioscaffold in bone tissue engineering

2022

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Autologous bone grafts are commonly used as the gold standard to repair and regenerate diseased bones. However, they are strongly associated with postoperative complications, especially at the donor site, and increased surgical costs. In an effort to overcome these limitations, tissue engineering (TE) has been proposed as an alternative to promote bone repair. The successful outcome of tissue engineering depends on the microstructure and composition of the materials used as scaffold. Decellularized bone matrix-based biomaterials have been applied as bioscaffolds in bone tissue engineering. These biomaterials play an important role in providing the mechanical and physical microenvironment needed by cells to proliferate and survive. Decellularized extracellular matrix (dECM) can be used as a powder, hydrogel and electrospun scaffolds. These bioscaffolds mimic the native microenvironment due to their structure similar to the original tissue. The aim of this review is to highlight the bone decellularization techniques. Herein we discuss: (1) bone structure; (2) properties of an ideal scaffold; (3) the potential of decellularized bone as bioscaffolds; (4) terminal sterilization of decellularized bone; (5) cell removing confirmation in decellularized tissues; and (6) post decellularization procedures. Finally, the improvement of bone formation by dECM and the immunogenicity aspect of using the decellularized bone matrix are presented, to illustrate how novel dECM-based materials can be used as bioscaffold in tissue engineering. A comprehensive understanding of tissue engineering may allow for better incorporation of therapeutic approaches in bone defects allowing for bone repair and regeneration.

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Section: Infrequent Clinical Trial Of Bioscaffolds In the Human Bodymentioning

confidence: 99%

Application of decellularized bone matrix as a bioscaffold in bone tissue engineering

2022

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“…Spongy granules were radiologically visible throughout the monitoring period, were responsible for the higher relative bone density values and underwent remodelling, but they adhered to the host bone to an insufficient extent, which delayed healing [ 41 , 42 ]. Karalashvili et al (2017) used a bovine bone graft for zygomatic bone reconstruction and reported that it retained its shape without resorption and bone integration [ 43 ]. Substantially better results were achieved by Kharkova et al (2019) after combining a tricalcium phosphate scaffold with EPO.…”

Section: Discussionmentioning

confidence: 99%

Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft

Vasileva

Chaprazov

2023

Veterinary Sciences

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Critical-size bone defect models are the standard in studies of the osteogenic potential of biomaterials. The present investigation aimed to evaluate the ability of recombinant human erythropoietin (EPO) to induce trabecular bone healing either alone or combined with a xenograft in a rat femoral critical-size defect model. Five-mm bone defects were created in the femoral diaphysis of fifty-six skeletally mature male Wistar albino rats. The animals were divided into six groups: one control group and five experimental groups. The defects in the control group were left empty, whereas an absorbable collagen cone soaked either with saline or erythropoietin (alone or in combination with xenograft) was placed in locally treated groups. The systemic treatment group received EPO subcutaneously. Bone formation was objectively evaluated through radiography, osteodensitometry and histological examination on post-operative days 30 and 90. The results demonstrate that EPO, locally applied on a collagen scaffold, was capable of inducing bone healing, whereas the single systemically administered high EPO dose had only an insignificant effect on bone formation. The combination of EPO with a bone substitute under the form of cancellous granules resulted in more rapid integration between the xenograft and host bone.

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“…Finally, xenografts have ethical and religious concerns. Karalashvili et al (2017) described the use of a decellularized bovine bone graft in a zygomatic large bone defect reconstruction and reported long-term retention of graft shape without resorption and bone integration. Bovine cancellous xenografts have also been used in the treatment of tibial fractures in elderly patients and showed favorable healing outcomes (Bansal et al, 2009).…”

Section: Xenograftsmentioning

confidence: 99%

Reconstruction of Large Skeletal Defects: Current Clinical Therapeutic Strategies and Future Directions Using 3D Printing

Vidal

Kampleitner

Brennan

et al. 2020

Front. Bioeng. Biotechnol.

137

108

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The healing of bone fractures is a well-orchestrated physiological process involving multiple cell types and signaling molecules interacting at the fracture site to replace and repair bone tissue without scar formation. However, when the lesion is too large, normal healing is compromised. These so-called non-union bone fractures, mostly arising due to trauma, tumor resection or disease, represent a major therapeutic challenge for orthopedic and reconstructive surgeons. In this review, we firstly present the current commonly employed surgical strategies comprising auto-, allo-, and xenograft transplantations, as well as synthetic biomaterials. Further to this, we discuss the multiple factors influencing the effectiveness of the reconstructive therapy. One essential parameter is adequate vascularization that ensures the vitality of the bone grafts thereby supporting the regeneration process, however deficient vascularization presents a frequently encountered problem in current management strategies. To address this challenge, vascularized bone grafts, including free or pedicled fibula flaps, or in situ approaches using the Masquelet induced membrane, or the patient's body as a bioreactor, comprise feasible alternatives. Finally, we highlight future directions and novel strategies such as 3D printing and bioprinting which could overcome some of the current challenges in the field of bone defect reconstruction, with the benefit of fabricating personalized and vascularized scaffolds.

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Decellularized Bovine Bone Graft for Zygomatic Bone Reconstruction

Cited by 8 publications

References 27 publications

Application of decellularized bone matrix as a bioscaffold in bone tissue engineering

Application of decellularized bone matrix as a bioscaffold in bone tissue engineering

Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft

Reconstruction of Large Skeletal Defects: Current Clinical Therapeutic Strategies and Future Directions Using 3D Printing

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