Chemical proteomics reveals HSP70 1A as a target for the anticancer diterpene oridonin in Jurkat cells

Bioactive Micronutrients in Coffee: Recent Analytical Approaches for Characterization and Quantification

“…A diterpene alkaloid isolated from the Caribbean sponge Agelas citrina has shown antifungal function [76]. Experiment with oridonin type has demonstrated its antiproliferative effect against leukemia-derived Jurkat cells [77]. Abietane type was cytotoxic to human pancreatic cancer cell line MIA PaCa-2 [78].…”

Natural products against hematological malignancies and identification of their targets

“…Dal Piaz et al [66] recently used a chemical proteomic approach to identify the molecular chaperone heat shock protein (HSP) 70 1A as an oridonin target in leukemic Jurkat cells. In detail, oridonin covalently binds to Cys 267 of HSP70 1A and inhibits its activity.…”

Hunting Molecular Targets for Anticancer Reagents by Chemical Proteomics

“…Moreover, the binding site of oridonin on the chaperone was identified by a mass-based approach combined with molecular dynamics simulations. 25 IV. NOVEL APPROACHES FOR CANCER THERAPIES…”

“…[23][24][25][26] Wang and coworkers 23 presented a biotinylated form of diazonamide A attached to agarose beads to unravel its molecular mechanism of perturbing spindle assembly in mammalian cell cultures. Binding profiles obtained for such strategy suggest that a mitochondrial enzyme ornithine d-amino transferase may be a primary target of diazonamide A.…”