Chemical Moieties and Interactions Involved in the Binding of Zearalenone to the Surface of <i>Lactobacillus rhamnosus</i> Strains GG

El-Nezami, Hani; Polychronaki, Nektaria; Lee, Yuan Kun; Haskard, Carolyn A.; Juvonen, Risto O.; Salminen, Seppo; Mykkänen, Hannu

doi:10.1021/jf049924m

Cited by 82 publications

(68 citation statements)

References 12 publications

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“…Overall, acid-treated cells bound less ZEN than the viable or autoclaved cells; however, this was not statistically significant (p<0.05). Nonetheless, this is a pointer to the presence, to a lesser extent of protein binding sites (El-Nezami et al 2004). …”

Section: Discussionmentioning

confidence: 99%

Adsorption and degradation of zearalenone by bacillus strains

Tinyiro

Wokadala

et al. 2011

Folia Microbiol

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Two Bacillus strains; Bacillus subtilis 168 and Bacillus natto CICC 24640 separately adsorbed and degraded zearalenone in liquid media, in vitro. Viable, autoclaved (121°C, 20 min) and acid-treated cells of both strains separately bound more than 55% of zearalenone (ZEN, 20 μg/L) after 30 min and 1-h incubation at 37°C under aerobic conditions, and the amount of ZEN adsorbed was dependent on initial cell volume. In addition, ZEN was degraded by the culture extract of both strains. Degradation by B. subtilis 168 and B. natto CICC 24640 culture extract after 24-h aerobic incubation at 30°C was 81% and 100%, respectively. B. natto CICC 24640 culture extract comprehensively degraded ZEN and, for both strains, no oestrogenic ZEN analogues were present. ZEN degradation was accompanied by carbondioxide emission indicating a decarboxylation reaction. ZEN degradation by the salient B. natto CICC 24640 culture extract varied with initial ZEN concentration, incubation time, temperature and pH. Degradation was enhanced by Mn(2+), Zn(2+), Ca(2+) and Mg(2+) but impeded by Hg(2+), Cu(2+), Pb(2+), ethylenediaminetetraacetic acid and 1,10-phenanthroline. The degradation reaction is associated with a metalloproteinase of molar mass in the range 31-43 kDa. Overall, the two generally recognised as safe Bacillus strains can, potentially, be utilised for detoxification of zearalenone in food.

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Section: Discussionmentioning

confidence: 99%

Adsorption and degradation of zearalenone by bacillus strains

Tinyiro

Wokadala

et al. 2011

Folia Microbiol

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“…ZEA is usually non-lethal to animals but due to its structural similarity with estradiol 17B (E 2 ), the main female sex hormone, they compete with each other for binding to the human cytosolic estrogenic receptor ERb in the uterus, mammary gland, hypothalamus and pituitary gland, influencing estrogen-dependent transcription in the nucleus (Bennett and Klich, 2003;Bräse et al, 2009;El-Nezami et al, 2004). Receptor binding by ZEA has been shown to inhibit the binding estrogenic hormones in rat mammary tissues.…”

Section: Introductionmentioning

confidence: 99%

Individual and combined cytotoxic effects of Fusarium toxins (deoxynivalenol, nivalenol, zearalenone and fumonisins B1) on swine jejunal epithelial cells

Wan¹,

Turner²,

El-Nezami³

2013

Food and Chemical Toxicology

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155

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“…The detoxification of PAT from aqueous solution by kombucha tea biomass could be explained on the basis of the adsorbent ability of bacteria and yeast cells present in the cellulose biomass of kombucha. Bacterial cell wall peptidoglycans and polysaccharides have been suggested to be responsible components for the mycotoxin binding by bacteria (El-Nezami et al, 2004). Also, yeast cell wall carbohydrates and proteins were reported to be important components involved in PAT removal (Guo et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Detoxification of Patulin by Kombucha tea culture

Ismaiel

Bassyouni

Kamel

et al. 2015

CyTA - Journal of Food

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Kombucha is a refreshing beverage, obtained by fermenting sugared tea with a symbiotic culture of acetic acid bacteria and yeast, consumed for its positive effects on human health. In this study, the potential of kombucha supernatant for reduction of patulin (PAT) of three toxigenic fungal strains (Penicillium expansum LC015096, Talaromyces purpureogenus LC015095, and Acremonium implicatum LC015097) in liquid medium and apple fruit was investigated. In liquid medium, kombucha up to 10% (v/v) significantly inhibited PAT production of P. expansum by 77.2% and that of T. purpureogenus and A. implicatum by 100%. In apple fruit, inhibition percent of PAT accumulation by the respective fungal strains was 49.8%, 100%, and 53%. In aqueous solution, kombucha cells showed a relative greater PAT uptake capacity than Sacchromyces cerevisiae. The maximum PAT uptake (64.67% and 60.69%) by viable and heat-treated kombucha cells was achieved at pH 3.0 throughout 48 h incubation, respectively.Keywords: Kombucha tea; patulin (PAT); toxicity; apple; biosorption La Kombucha es una bebida refrescante obtenida a partir de la fermentación del té endulzado con un cultivo simbiótico de acetobacterias y levadura, el cual es consumido por sus efectos positivos para la salud. En este estudio, se investigó el potencial de la Kombucha sobrenadante para la reducción de patulina (PAT) de tres cepas de hongos tóxicos (Penicillium expansum LC015096, Talaromyces purpureogenus LC015095 y Acremonium implicatum LC015097) en medio líquido y zumo de manzana. En medio líquido, la Kombucha con un porcentaje de hasta 10% (v/v) inhibió significativamente la producción de PAT de P. expansum en un 77,2% y la de T. purpureogenus y A. implicatum en un 100%. En el zumo de manzana, el porcentaje de inhibición de la acumulación de PAT mediante las cepas de hongos respectivas fue de 49,8%, 100% y 53%. En la solución acuosa, las células de Kombucha mostraron una mayor capacidad relativa de absorción de PAT que en Sacchromyces cerevisiae. La capacidad máxima de absorción de PAT (64,67% y 60,69%) mediante células de Kombucha viables y tratadas con calor se consiguió con un pH 3,0 mediante incubación de 48 h, respectivamente.

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Chemical Moieties and Interactions Involved in the Binding of Zearalenone to the Surface of Lactobacillus rhamnosus Strains GG

Cited by 82 publications

References 12 publications

Adsorption and degradation of zearalenone by bacillus strains

Adsorption and degradation of zearalenone by bacillus strains

Individual and combined cytotoxic effects of Fusarium toxins (deoxynivalenol, nivalenol, zearalenone and fumonisins B1) on swine jejunal epithelial cells

Detoxification of Patulin by Kombucha tea culture

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