Chemical modifications of achromobacter collagenase and their influence of the enzymic activity

Trocheris, I.; Herry, P.; Keil-Dlouhá, V.; Keil, B.

doi:10.1016/0005-2744(80)90510-0

Cited by 5 publications

(2 citation statements)

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“…Previous studies have shown that the subunit of Achromobacter collagenase (strain 1.029) may be structurally similar to the neutral proteinases recovered from Bacillus thermoproteolyticus (thermolysin; EC 3.4.24.4) and B. subtilis (10). This association is based on a comparison of molecular weights, amino acid compositions (9,14,32), essential components of the enzyme active sites (7,33), and circular dichroism measurements (6). However, a comparison of the amino acid compositions of the purified subunits of strain CIP 82.01 and 1.029 collagenases with the amino acid compositions of thermolysin and B .…”

Section: Discussionmentioning

confidence: 99%

Assignment of "Achromobacter iophagus" Strain I.029 to Vibrio alginolyticus Chemovar iophagus

Emod

Soubigou

Tong

et al. 1983

International Journal of Systematic Bacteriology

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RICHARD^Strain CIP 82.01 of Vibrio alginolyticus and strain 1.029 (previously designated "Achromobacter iophagus") were compared. Strain 1.029 produces a collagenase of high specific activity (Achrornobacter collagenase; EC 3.4.24.8). Collagenase production is induced in strain CIP 82.01 by collagen or macromolecular fragments of collagen in a manner similar to collagenase induction in strain 1.029; caseinolytic proteinase is constitutive. In this study we demonstrate that both strains also produce a constitutive extracellular endonuclease. Collagenases from both strains cleave either native collagen in its helical region or a similar synthetic peptide; both enzymes are inhibited by ethylenediamine tetraacetate, but not by diisopropyl fluorophosphate. The collagenase subunit (molecular weight, 35,000) of strain CIP 82.01 is similar in amino acid composition to the subunit of the strain 1.029 enzyme, although some of the aspartic and threonine residues in strain CIP 82.01 are replaced by glutamic and serine residues in strain 1.029. Surface radioiodination followed by two-dimensional electrophoresis showed that there are quantitative differences in the major outer membrane proteins of the two strains. Strains CIP 82.01 and 1.029 differ qualitatively in resistance to ampicillin and carbenicillin, in cellobiose fermentation, in ornithine decarboxylase activity, and in halophilism. We propose that strain 1.029, which was originally designated A . iophagus, be included within the species V. alginolyticus, but that this organism be distinguished from other strains of this species by the designation Vibrio alginolyticus chemovar iophagus, with the corresponding collagenase designated "iophagus collagenase" (EC 3.4.24.8).In 1973, Welton and Woods isolated and described the gram-negative aerobic bacterium Achromobacter iophagus (34). In the presence of collagen or macromolecular fragments of collagen (15), a subculture of this organism (strain 1.029) produces a collagenase having a very high specific activity (12,20). The structural and functional properties of this Achromobacter collagenase (EC 3.4.24.8), as well as the metabolism of strain 1.029, have been described in a series of papers by Woods et al. (21,26,27,29,35) and by workers in our laboratory (4,10,11,13,14,19,30).In a recent study (27), which confirmed the original observation of collagenase induction by macromolecular collagen fragments (15), Achromobacter iophagus was reclassified as a strain of Vibrio alginolyticus; however, a direct experimental comparison of strain 1.029 with a welldefined wild-type strain of V . alginolyticus has been lacking. It is well established that the other strains of the Vibrionaceae (23, 31) can be induced (by collagen and fragments of collagen) to produce collagenolytic and gelatinolytic enzymes.We undertook a comparative study to determine the differences between strain 1.029 isolated from a random selection of cured hides in South Africa (34) and a wild-type strain (strain CIP 82.01) of V . alginolyticus from a different ge...

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Section: Discussionmentioning

confidence: 99%

Assignment of "Achromobacter iophagus" Strain I.029 to Vibrio alginolyticus Chemovar iophagus

Emod

Soubigou

Tong

et al. 1983

International Journal of Systematic Bacteriology

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“…Comparison of amino acid composition In a previous study, Trocheris et al [28] reported the amino acid composition of V. alginolyticus collagenase from a chemically purified protein sample. The comparison of the two amino acid compositions is shown in Table 3.…”

Section: Elisa With Anti-peptide Antibodiesmentioning

confidence: 99%

Structural gene and complete amino acid sequence of Vibrio alginolyticus collagenase

Takeuchi

Shibano

Morihara

et al. 1992

Biochemical Journal

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The DNA encoding the collagenase of Vibrio alginolyticus was cloned, and its complete nucleotide sequence was determined. When the cloned gene was ligated to pUC 18, the Escherichia coli expression vector, bacteria carrying the gene exhibited both collagenase antigen and collagenase activity. The open reading frame from the ATG initiation codon was 2442 bp in length for the collagenase structural gene. The amino acid sequence, deduced from the nucleotide sequence, revealed that the mature collagenase consists of 739 amino acids with an Mr of 81 875. The amino acid sequences of 20 polypeptide fragments were completely identical with the deduced amino acid sequences of the collagenase gene. The amino acid composition predicted from the DNA sequence was similar to the chemically determined composition of purified collagenase reported previously. The analyses of both the DNA and amino acid sequences of the collagenase gene were rigorously performed, but we could not detect any significant sequence similarity to other collagenases.

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Enzyme Handbook

Schomburg¹,

Salzmann²

1991

Enzyme Handbook

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Chemical modifications of achromobacter collagenase and their influence of the enzymic activity

Cited by 5 publications

References 27 publications

Assignment of "Achromobacter iophagus" Strain I.029 to Vibrio alginolyticus Chemovar iophagus

Assignment of "Achromobacter iophagus" Strain I.029 to Vibrio alginolyticus Chemovar iophagus

Structural gene and complete amino acid sequence of Vibrio alginolyticus collagenase

Enzyme Handbook

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