Chemical labels and natural element tags for the quantitative analysis of bio-molecules

Inductively coupled plasma- and glow discharge plasma-sector field mass spectrometry : Part II. Applications

“…[306][307][308] This is especially true for the characterization of metal containing or metal-binding proteins in biological systems (see the new Royal Society of Chemistry journal-Metallomics) and for protein modifications involving 'heteroelements' such as protein phosphorylation. 309,310 Concerning metals, the contribution from spectral interferences is strongly dependent on the matrix (for instance in case of V, Cr, Cu and Zn). Elements such Fe, Mn and Se are always spectrally interfered.…”

Time-resolved ICP-MS Measurement: a New Method for Elemental and Multiparametric Analysis of Single Cells

“…Not only metal-containing biomolecules, but also other biomolecules, such as amino acids, peptides, proteins, nucleic acids and microbial cells, can be successfully quantified by measuring heteroatoms, such as phosphorus and sulfur, and/or by supporting elemental tags, such as metal-labeled antigens and nanoparticles. [4][5][6][7][8][9][10] In recent years, cytometric analysis of single cells with a time-resolved ICP-MS measurement is receiving much attention for elemental [14][15][16][17][18] and multiparametric [19][20][21][22][23] analyses of single cells. Due to cellular heterogeneity within an isogenic cell population, individual analysis of cells will lead to a more sensitive representation of cell-to-cell variations, instead of a stochastic average analysis by bulk measurements.…”

Preparation of Metal-p-aminobenzyl-DOTA Complex Using Magnetic Particles for Bio-tagging in Laser Ablation ICP-MS

“…6,11,12 It forms complexes with Ln (III) ions that are thermodynamically stable and kinetically inert 12,13 ; it is used for the diagnosis and therapy of tumors in nuclear medicine 14 and in MRI contrast agent Dotarem. [15][16][17] Both its formation and dissociation rates are much lower than those of acyclic polyamino polycarboxylates, such as EDTA.…”

“…Such sampling involves the immobilization of a sample on the chip followed by metal tagging. [5][6][7][8][9] For this, the tagged material should be firmly attached and stable against washing and storage without sample loss. This can be achieved through chemical bonding rather than intermolecular interactions.…”