Chemical investigation ofHippophaë rhamnoides. II. Main components of the neutral reaction of the saponification products of an extract of the leaves of the sea buckthorn

Salenko, V. L.; Кукина, Т. П.; Karamyshev, V. N.; Sidelnikov, V. N.; Pentegova, V. A.

doi:10.1007/bf00579142

Cited by 7 publications

(2 citation statements)

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“…Goncharova and Glushenkova (1996) reported that sea buckthorn leaves were rich in carotenoids, free and esterified sterols, triterpenols, and isoprenols. Numerous products are made from sea buckthorn leaves such as leaf extract (Salenko et al, 1986), tea, tea powder, and animal feed.…”

Section: Introductionmentioning

confidence: 99%

Sea Buckthorn Products: Manufacture and Composition

Beveridge

Oomah

et al. 1999

J. Agric. Food Chem.

276

204

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Sea buckthorn (Hippophae rhamnoides) is a unique plant currently being domesticated. The fruit is the main component of value, although the leaves are occasionally made into sea buckthorn tea. The two main sources of valuable products are derived from the berries, juice from the fleshy tissue and seed as a single seed from each berry. The juice provides a nutritious beverage, high in suspended solids and very high in vitamin C and carotenes. The juice may contain an oil phase trapped within the suspended solids, or the oil may be removed as pulp oil and provided separately. The pulp remaining after juice removal provides for extraction of "sea buckthorn yellow", a pigment that has potential use as a food coloring material. The seed is a source of seed oil, which is very unsaturated and shows promise, because of its light absorption and emollient properties, as an ingredient in cosmetics, phytopharmaceuticals, or UV skin protectant preparations. It may be prepared by conventional extraction techniques or by supercritical carbon dioxide extraction. The manufacture of the main products derived from sea buckthorn is described, including several examples from the patent literature. The available compositional data for the main products are tabulated to form a comprehensive source of information on the manufacture and composition of sea buckthorn products.

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Section: Introductionmentioning

confidence: 99%

Sea Buckthorn Products: Manufacture and Composition

Beveridge

Oomah

et al. 1999

J. Agric. Food Chem.

276

204

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“…1) Investigations of H. rhamnoides have yielded flavonols, [2][3][4] triterpenoids, 5,6) and tannins.…”

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Inhibitory Effects of the Constituents of <i>Hippophae rhamnoides</i> on 3T3-L1 Cell Differentiation and Nitric Oxide Production in RAW264.7 Cells

Yang

Wen

et al. 2013

Chem. Pharm. Bull.

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Three new flavonol glycosides, hippophaeosides A-C (1-3), together with 27 known constituents, were isolated from Hippophae rhamnoides L. leaves. Their structures were determined by spectroscopic analyses. Their inhibitory activities on 3T3-L1 preadipocyte differentiation and triglyceride accumulation in maturing adipocytes, and nitric oxide production in RAW264.7 cells were examined.Key words Hippophae rhamnoides; flavonol glycoside; triterpenoid; adipocyte; macrophage Hippophae rhamnoides L. (Elaeagnaceae), known as "sea buckthorn," is a deciduous shrub distributed throughout Asia and Europe. The fruit has been used as a traditional medicine for the treatment of cough indigestion and blood stasis in China.1) Investigations of H. rhamnoides have yielded flavonols, 2-4) triterpenoids, 5,6) and tannins.3) The leaf has been reported to have anti-inflammatory, 7) antioxidant, 4) anti-obesity, 8,9) and α-glucosidase inhibitory activities. 4)In our previous study, we investigated anti-obesity and antiinflammatory compounds that not only inhibit triglyceride (TG) accumulation by 3T3-L1 adipocytes but also inhibit the secretion of nitric oxide (NO) from RAW264.7 cells. In that study, several crude drugs were examined. [10][11][12] In the present study, the 80% methanol extract of the leaves of H. rhamnoides L. and the chloroform-and ethyl acetatesoluble portions of the extract were observed to inhibit the TG accumulation in 3T3-L1 cells, and inhibit NO production in lipopolysaccharide (LPS) and interferon-γ (IFN-γ) activated macrophages. Furthermore, an attempt to identify the bioactive compounds present in this plant led to the isolation of three new flavonol glycosides, Hippophaeosides A (1), B (2), and C (3), along with 27 known constituents. Results and DiscussionThe leaves of H. rhamnoides L. were extracted with 80% aqueous methanol, followed by evaporation of the solvent under reduced pressure from the combined extract to give the 80% methanol extract. The extract [TG and NO inhibitory activity: 35% and 63%, respectively (30 µg/mL) [42, 15% (30 µg/mL)]. We focused our efforts on investigating the chemical constituents of the chloroform-and ethyl acetate-soluble fractions. The ethyl acetate layer was separated by Sephadex LH-20 column chromatography to yield four fractions from which 22 compounds including three new compounds 1-3 were isolated. Eight compounds were isolated from the chloroform layer. Compounds 4-30 were identified by detailed comparisons of their

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