Chemical Investigation of Carrageenan from the Red alga <i>Sarconema filiforme</i> (Gigartinales, Rhodophyta) of Indian Waters

Kumar, Sanjay; Mehta, Gaurav K.; Prasad, Kamalesh; Meena, Ramavtar; Siddhanta, A. K.

doi:10.1177/1934578x1100600928

Cited by 8 publications

(3 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Natural polysaccharides rarely have a regular structure. As a rule, they are built up from disaccharide units of several carrageenan types with a predominance of one or another type [6][7][8], or they are even more complex D/L-hybrid structures that contain agar and carrageenan units [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Carrageenans and Their Oligosaccharides from Red Seaweeds Ahnfeltiopsis flabelliformis and Mastocarpus pacificus (Phyllophoraceae) and Their Antiproliferative Activity

Kravchenko

Menchinskaya

Исаков

et al. 2023

IJMS

View full text Add to dashboard Cite

Comparative structural analysis of gelling polysaccharides from A. flabelliformis and M. pacificus belonging to Phyllophoraceae and the effect of their structural features and molecular weight on human colon cancer cell lines (HT-29, DLD-1, HCT-116) was carried out. According to chemical analysis, IR and NMR spectroscopies, M. pacificus produces kappa/iota-carrageenan with a predominance of kappa units and minor amounts of mu and/or nu units, while the polysaccharide from A. flabelliformis is iota/kappa-carrageenan (predominance of iota units) and contains negligible amounts of beta- and nu-carrageenans. Iota/kappa- (Afg-OS) and kappa/iota-oligosaccharides (Mp-OS) were obtained from the original polysaccharides through mild acid hydrolysis. The content of more sulfated iota units in Afg-OS (iota/kappa 7:1) was higher than in Mp-OS (1.0:1.8). The poly- and oligosaccharides up to 1 mg/mL did not show a cytotoxic effect on all tested cell lines. Polysaccharides showed an antiproliferative effect only at 1 mg/mL. Oligosaccharides had a more pronounced effect on HT-29 and HCT-116 cells than the original polymers, while HCT-116 cells were slightly more sensitive to their action. Kappa/iota-oligosaccharides exhibit a greater antiproliferative effect and more strongly decrease the number of colonies forming in HCT-116 cells. At the same time, iota/kappa-oligosaccharides inhibit cell migration more strongly. Kappa/iota-oligosaccharides induce apoptosis in the SubG0 and G2/M phases, while iota/kappa-oligosaccharides in the SubG0 phase.

show abstract

Section: Introductionmentioning

confidence: 99%

Carrageenans and Their Oligosaccharides from Red Seaweeds Ahnfeltiopsis flabelliformis and Mastocarpus pacificus (Phyllophoraceae) and Their Antiproliferative Activity

Kravchenko

Menchinskaya

Исаков

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…α-Carrageenan has been observed in the cell wall of several red algae such as Catenella nipae Zanardini (Zablackis and Santos, 1986), Sarconema filiforme (Sonder) Kylin (Chiovitti et al, 1998; Kumar et al, 2011) and in some Solieria spp. (Chiovitti et al, 1997; Bondu et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Discovery of a novel iota carrageenan sulfatase isolated from the marine bacterium Pseudoalteromonas carrageenovora

Genicot¹,

Groisillier²,

Rogniaux³

et al. 2014

Front. Chem.

View full text Add to dashboard Cite

Carrageenans are sulfated polysaccharides extracted from the cell wall of some marine red algae. These polysaccharides are widely used as gelling, stabilizing, and viscosifying agents in the food and pharmaceutical industries. Since the rheological properties of these polysaccharides depend on their sulfate content, we screened several isolated marine bacteria for carrageenan specific sulfatase activity, in the aim of developing enzymatic bioconversion of carrageenans. As a result of the screening, an iota-carrageenan sulfatase was detected in the cell-free lysate of the marine bacterium Pseudoalteromonas carrageenovora strain PscT. It was purified through Phenyl Sepharose and Diethylaminoethyl Sepharose chromatography. The pure enzyme, Psc ι-CgsA, was characterized. It had a molecular weight of 115.9 kDaltons and exhibited an optimal activity/stability at pH ~8.3 and at 40 ± 5°C. It was inactivated by phenylmethylsulfonyl fluoride but not by ethylene diamine tetraacetic acid. Psc ι-CgsA specifically catalyzes the hydrolysis of the 4-S sulfate of iota-carrageenan. The purified enzyme could transform iota-carrageenan into hybrid iota-/alpha- or pure alpha-carrageenan under controlled conditions. The gene encoding Psc ι-CgsA, a protein of 1038 amino acids, was cloned into Escherichia coli, and the sequence analysis revealed that Psc ι-CgsA has more than 90% sequence identity with a putative uncharacterized protein Q3IKL4 from the marine strain Pseudoalteromonas haloplanktis TAC 125, but besides this did not share any homology to characterized sulfatases. Phylogenetic studies show that P. carrageenovora sulfatase thus represents the first characterized member of a new sulfatase family, with a C-terminal domain having strong similarity with the superfamily of amidohydrolases, highlighting the still unexplored diversity of marine polysaccharide modifying enzymes.

show abstract

“…Then the derivatives are analysed by GC-MS, and the composition and linkage pattern are analysed by comparing the spectral data with the Carbohydrate Research Centre Spectral Database-PMAA. 103,104,179 For example, the α, ı, and pyruvated carrageenan content and position of sulphate esters in the carrageenan from the red alga Sarconema filiforme were determined by linkage analysis by GC-MS. 105 Similarly, the nature of the glycosidic linkages and sulphate positions in the extracted sulphated polysaccharides from Ulva pertusa were elucidated by methylation analysis using GC-MS. 106…”

Section: Structural Characterization Methodsmentioning

confidence: 99%

Seaweed-based polysaccharides – review of extraction, characterization, and bioplastic application

Krishnan,

Ravi,

Kumar Mondal

et al. 2024

Green Chem.

View full text Add to dashboard Cite

show abstract

Chemical Investigation of Carrageenan from the Red alga Sarconema filiforme (Gigartinales, Rhodophyta) of Indian Waters

Cited by 8 publications

References 47 publications

Carrageenans and Their Oligosaccharides from Red Seaweeds Ahnfeltiopsis flabelliformis and Mastocarpus pacificus (Phyllophoraceae) and Their Antiproliferative Activity

Carrageenans and Their Oligosaccharides from Red Seaweeds Ahnfeltiopsis flabelliformis and Mastocarpus pacificus (Phyllophoraceae) and Their Antiproliferative Activity

Discovery of a novel iota carrageenan sulfatase isolated from the marine bacterium Pseudoalteromonas carrageenovora

Seaweed-based polysaccharides – review of extraction, characterization, and bioplastic application

Contact Info

Product

Resources

About