Chemical imaging of the reconstruction of new bone and trace elements inside bioactive glass scaffolds in vivo: a multimodal and quantitative micro‐ion beam analysis

Abstract

An original multimodal analysis is proposed for follow‐up of bone regeneration inside a SiO2–CaO bioactive glass scaffold in vivo. Classic histological studies are conducted in connection with SEM with backscattered electron imaging and micro‐particle induced X‐ray emission ion beam analysis, allowing fine morphological characterization and highly sensitive microanalysis of the scaffold/bone interface. The highly bioactive scaffold is entirely changed into bone mineral after 6 weeks of implantation in sheep mandible; thanks to our proposed methodology, it is possible to highlight subtle differences in mineralization and to distinguish between the mineralized scaffold, the newly formed bone and native bone from the trace metal nutrients content. Copyright © 2014 John Wiley & Sons, Ltd.

A review: In vivo studies of bioceramics as bone substitute materials

“…Nevertheless, Lao et al. [ 119 ] investigated based on the multimodal study of SiO 2 –CaO bioactive glass scaffolds implanted in the mandible of sheep by follow‐up of differences in bone minerals. The result provides helpful details on bone maturity and bone mineral construction.…”

“…After 11 weeks, it showed very useful in renewing a rat tibia defect by bone generation in the pore network and degradation of the scaffold. Nevertheless, Lao et al [119] investigated based on the multimodal study of SiO 2 -CaO bioactive glass scaffolds implanted in the mandible of sheep by follow-up of differences in bone minerals. The result provides helpful details on bone maturity and bone mineral construction.…”