Chemical genetics and strigolactone perception

Lumba, Shelley; Bunsick, Michael; McCourt, Peter

doi:10.12688/f1000research.11379.1

Cited by 7 publications

(5 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We postulate that the plant SCF D3-D14 complex has evolved these unusual features to orchestrate SL sensing, substrate poly-ubiquitination, and hormone metabolism in a highly coordinated manner. To explain the activity of non-hydrolyzable SL agonists 6,33–35 , we propose that D14 perceives and transduces the hormonal signal in its open conformation, which is recognized by the D3-CTH and is competent for D53 binding (Extended Data Fig. 8, Supplementary Discussion).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Structural plasticity of D3–D14 ubiquitin ligase in strigolactone signalling

Shabek

Ticchiarelli

Mao

et al. 2018

Nature

147

183

View full text Add to dashboard Cite

SUMMARY The plant hormone strigolactones (SLs) regulate many aspects of plant physiology. In shoot branching inhibition, the SL-metabolizing α/β hydrolase D14 interacts with the F-box protein D3 to ubiquitinate and degrade the transcription repressor D53. Despite multiple modes of D14-SL interactions determined recently, how the hydrolase functions with D3 to mediate hormone-dependent D53 ubiquitination remains elusive. Here we show that D3 features a C-terminal α-helix (CTH), which can switch between two conformational states. Distinct from its engaged form, which facilitate the binding of D3 and D14 with a hydrolyzed SL intermediate, the dislodged D3 CTH can recognize unmodified D14 in an open conformation and inhibits its enzymatic activity. In an SL-dependent manner, the D3 CTH enables D14 to recruit D53, which in turn activates the hydrolase. By unraveling an unexpected structural plasticity in SCF D3-D14 ubiquitin ligase, our results suggest an intricate mechanism by which the E3 coordinates SL signaling and metabolism.

show abstract

Section: Resultsmentioning

confidence: 99%

“…These results raised the possibility that CLIM might represent the active form of the hormone. This proposition, however, is complicated by the identification of multiple non-hydrolyzable SL agonists 6,33–35 .…”

Section: Introductionmentioning

confidence: 99%

Structural plasticity of D3–D14 ubiquitin ligase in strigolactone signalling

Shabek

Ticchiarelli

Mao

et al. 2018

Nature

147

183

View full text Add to dashboard Cite

show abstract

“…Therefore, chemical regulators of SL functions might ultimately achieve widespread use in agricultural applications (Nakamura and Asami, 2014). To date, SL mimics have been developed to inhibit branching (Nakamura and Asami, 2014;Lumba et al, 2017a) and trigger suicidal germination of parasitic weeds, and some compounds have succeeded in generating practical treatments that can induce suicidal germination of parasitic weeds in soil (Samejima et al, 2016). Recently, several groups have reported the development of SL antagonists (Holbrook-Smith et al, 2016; Mashita et al, 2016;Xiang et al, 2017;Hameed et al, 2018;Hamiaux et al, 2018;Takeuchi et al, 2018;Yoshimura et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Triazole Ureas Covalently Bind to Strigolactone Receptor and Antagonize Strigolactone Responses

et al. 2019

View full text Add to dashboard Cite

Strigolactones, a class of plant hormones with multiple functions, mediate plant-plant and plantmicroorganism communications in the rhizosphere. In this study, we developed potent strigolactone antagonists, which covalently bind to the strigolactone receptor D14, by preparing an array of triazole urea compounds. Using yeast two-hybrid and rice-tillering assays, we identified a triazole urea compound KK094 as a potent inhibitor of strigolactone receptors. Liquid chromatography-tandem mass spectrometry analysis and X-ray crystallography revealed that KK094 was hydrolyzed by D14, and that a reaction product of this degradation covalently binds to the Ser residue of the catalytic triad of D14. Furthermore, we identified two triazole urea compounds KK052 and KK073, whose effects on D14-D53/D14-SLR1 complex formation were opposite due to the absence (KK052) or presence (KK073) of a trifluoromethyl group on their phenyl ring. These results demonstrate that triazole urea compounds are potentially powerful tools for agricultural application and may be useful for the elucidation of the complicated mechanism underlying strigolactone perception.

show abstract

“…Host-derived signal substances, or “host factors,” control the organ development processes of parasites. A well-known example of the host factors are strigolactones, that are exuded from host root, that trigger germination of seeds of Orobancaceae plants (for reviews, see Xie et al, 2010; Lumba et al, 2017). In the case of Cuscuta , volatiles emitted from the host is known to mediate host location by Cuscuta (Runyon et al, 2006).…”

Section: Interaction With Hostmentioning

confidence: 99%

Development of Parasitic Organs of a Stem Holoparasitic Plant in Genus Cuscuta

Shimizu

Aoki

2019

Front. Plant Sci.

View full text Add to dashboard Cite

Parasitic plants infect a broad range of plant species including economically important crops. They survive by absorbing water, minerals, and photosynthates from their hosts. To support their way of life, parasitic plants generally establish parasitic organs that allow them to attach to their hosts and to efficiently absorb substances from the vascular system of the host. Here, we summarize the recent progress in understanding the mechanisms underlying the formation of these parasitic organs, focusing on the process depicted in the stem holoparasitic genus, Cuscuta. An attachment structure called “holdfast” on the stem surface is induced by the light and contact stimuli. Concomitantly with holdfast formation, development of an intrusive structure called haustorium initiates in the inner cortex of the Cuscuta stem, and it elongates through apoplastic space of the host tissue. When haustoria reaches to host vascular tissues, they begin to form vascular conductive elements to connect vascular tissue of Cuscuta stem to those of host. Recent studies have shown parasite-host interaction in the interfacial cell wall, and regulation of development of these parasitic structures in molecular level. We also briefly summarize the role of host receptor in the control of compatibility between Cuscuta and hosts, on which occurrence of attachment structure depends, and the role of plant-to-plant transfer of long-distance signals after the establishment of conductive structure.

show abstract

Chemical genetics and strigolactone perception

Cited by 7 publications

References 72 publications

Structural plasticity of D3–D14 ubiquitin ligase in strigolactone signalling

Structural plasticity of D3–D14 ubiquitin ligase in strigolactone signalling

Triazole Ureas Covalently Bind to Strigolactone Receptor and Antagonize Strigolactone Responses

Development of Parasitic Organs of a Stem Holoparasitic Plant in Genus Cuscuta

Contact Info

Product

Resources

About