Structural plasticity of D3–D14 ubiquitin ligase in strigolactone signalling

Shabek, Nitzan; Ticchiarelli, Fabrizio; Mao, Haibin; Hinds, Thomas R.; Leyser, Ottoline; Zheng, Ning

doi:10.1038/s41586-018-0743-5

Cited by 147 publications

(200 citation statements)

References 39 publications

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“…However, recent findings demonstrated that specific binding of SL to D14 is enough to induce conformational change of D14 to interact with other components from the SL signaling pathway. [114][115][116] This model can explain why compounds like debranones (21), which lack the site of hydrolysis, are active SL agonists. Hydrolytic degradation of SLs by D14 may help maintain homeostatic level of SLs.…”

Section: Perception and Signal Transductionmentioning

confidence: 99%

Recent progress in the chemistry and biochemistry of strigolactones

Yoneyama

2020

J. Pestic. Sci.

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Strigolactones (SLs) are plant secondary metabolites derived from carotenoids. SLs play important roles in the regulation of plant growth and development in planta and coordinate interactions between plants and other organisms including root parasitic plants, and symbiotic and pathogenic microbes in the rhizosphere. In the 50 years since the discovery of the first SL, strigol, our knowledge about the chemistry and biochemistry of SLs has advanced explosively, especially over the last two decades. In this review, recent advances in the chemistry and biology of SLs are summarized and possible future outcomes are discussed.

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Section: Perception and Signal Transductionmentioning

confidence: 99%

Recent progress in the chemistry and biochemistry of strigolactones

Yoneyama

2020

J. Pestic. Sci.

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“…A recent study has provided new insights into the formation of the rice D14-D53-SCF D3 complex (Shabek et al, 2018). The crystal structure of the D3-ASK1 complex revealed the Cterminal a-helix (CTH) of D3 is dynamic and exists in two forms, dislodged from, or engaged with the D3-ASK1 complex (Shabek et al, 2018). This D3-CTH peptide was shown to directly interact with D14 in vitro and in planta in a GR24-dependent manner (Shabek et al, 2018).…”

Section: Reviewmentioning

confidence: 99%

“…The crystal structure of the D3-ASK1 complex revealed the Cterminal a-helix (CTH) of D3 is dynamic and exists in two forms, dislodged from, or engaged with the D3-ASK1 complex (Shabek et al, 2018). This D3-CTH peptide was shown to directly interact with D14 in vitro and in planta in a GR24-dependent manner (Shabek et al, 2018). D3-CTH uses common residues to interact with both D3-ASK1 and D14, suggesting it binds to D14 when dislodged from D3-ASK1 (Shabek et al, 2018).…”

Section: Reviewmentioning

confidence: 99%

Fellowship of the rings: a saga of strigolactones and other small signals

2019

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Strigolactones are an important class of plant signalling molecule with both external rhizospheric and internal hormonal functions in flowering plants. The past decade has seen staggering progress in strigolactone biology, permitting highly detailed understanding of their signalling, synthesis and biological rolesor so it seems. However, phylogenetic analyses show that strigolactone signalling mediated by the D14-SCF MAX2-SMXL7 complex is only one of a number of closely related signalling pathways, and is much less ubiquitous in land plants than might be expected. The existence of closely related pathways, such as the KAI2-SMAX1 module, challenges many of our assumptions about strigolactones, and in particular emphasises how little we understand about the specificity of strigolactone signalling with respect to related signalling pathways. In this review, we examine recent advances in strigolactone signalling, taking a holistic evolutionary view to identify the ambiguities and uncertainties in our understanding. We highlight that while we now have highly detailed molecular models for the core mechanism of D14-SMXL7 signalling, we still do not understand the ligand specificity of D14, the specificity of its interaction with SMXL7, nor the specificity of SMXL7 function. Our analysis therefore identifies key areas requiring further study.

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“…Biochemical analyses with recombinant D14 proteins by means of the synthetic SL analog GR24 revealed that the SL signal transduction requires GR24 cleavage 11,12 . One of the cleavage products, the D ring, may remain covalently attached to the receptor 13,14 , thereby probably allowing the recruitment of partners for downstream processes 15,16 . The required SL cleavage for signal transduction is still under debate 15,16 .…”

Section: Introductionmentioning

confidence: 99%

“…One of the cleavage products, the D ring, may remain covalently attached to the receptor 13,14 , thereby probably allowing the recruitment of partners for downstream processes 15,16 . The required SL cleavage for signal transduction is still under debate 15,16 .…”

Section: Introductionmentioning

confidence: 99%

APhelipanche ramosaKAI2 Protein Perceives enzymatically Strigolactones and Isothiocyanates

Germain¹,

Jacobs²,

Brun³

et al. 2020

Preprint

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22Phelipanche ramosa is an obligate root-parasitic weed threatening major crops in central 23Europe. For its germination, it has to perceive various structurally diverging host-exuded 24 signals, including isothiocyanates (ITCs) and strigolactones (SLs). However, the receptors 25 involved are still uncharacterized. Here, we identified five putative SL receptors in P. ramosa, 26 of which PrKAI2d3 is involved in seed germination stimulation. We established the high 27 plasticity of PrKAI2d3, allowing interaction with different chemicals, including ITCs. The SL 28 perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic 29 plants. We provide evidence that the PrKAI2d3 enzymatic activity confers hypersensitivity to 30SLs. Additionally, we demonstrated that methylbutenolide-OH binds PrKAI2d3 and 31 stimulates P. ramosa germination with a bioactivity comparable to that of ITCs. This study 32 highlights that P. ramosa has extended its signal perception system during evolution, a fact to 33 be considered in the development of specific and efficient biocontrol methods. 34Besides their involvement in germination, SLs have a strong stimulating activity on 54 arbuscular mycorrhizal fungi (Glomeromycotina) by promoting mitochondrial metabolism 55 and hyphal branching 7 , thereby mediating the establishment of the oldest mutualistic 56 interaction of land plants 8 . In addition to their role in rhizosphere signaling, SLs act as 57 hormones in planta with pervasive roles throughout the plant development ,9 and, as such, are 58 perceived in vascular plants by the α/β-hydrolase DWARF14 (D14) 10,11 . Biochemical 59 analyses with recombinant D14 proteins by means of the synthetic SL analog GR24 revealed 60 that the SL signal transduction requires GR24 cleavage 11,12 . One of the cleavage products, the 61 D ring, may remain covalently attached to the receptor 13,14 , thereby probably allowing the 62 recruitment of partners for downstream processes 15,16 . The required SL cleavage for signal 63 transduction is still under debate 15,16 . 64 In Arabidopsis thaliana, D14 belongs to a small gene family, including KARRIKIN 65 INSENSITIVE2/HYPOSENSITIVE TO LIGHT (AtKAI2/AtHTL) that shares the α/β-66 hydrolase catalytic triad. However, AtKAI2 regulates AtD14-independent processes, such as 67 seed germination, and preferentially perceives (−)-GR24 that mimics non-natural SLs, 68 karrikins 17 , and supposedly a still unknown endogenous ligand 18 . Interestingly, the KAI2 gene 69 family has expanded during the evolution of obligate parasitic-plant genomes 19 . For example, 70Phelipanche aegyptiaca and Striga hermonthica possess five and eleven KAI2/HTL genes, 71 respectively. Two KAI2 paralogs play a role in SL response in P. aegyptiaca 19 and six S. 72 hermonthica KAI2/HTL proteins are hypersensitive to SLs 19,20 , with ShHTL7 exhibiting the 73 same perception mechanism as D14 21 . The remarkable expansion of the KAI2 gene family in 74Orobanchaceae along with the capacity of P. ramosa to perceive ITCs let us...

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Structural plasticity of D3–D14 ubiquitin ligase in strigolactone signalling

Cited by 147 publications

References 39 publications

Recent progress in the chemistry and biochemistry of strigolactones

Recent progress in the chemistry and biochemistry of strigolactones

Fellowship of the rings: a saga of strigolactones and other small signals

APhelipanche ramosaKAI2 Protein Perceives enzymatically Strigolactones and Isothiocyanates

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