Chemical fingerprint and metabolic fingerprint analysis of Danshen injection by HPLC–UV and HPLC–MS methods

Zhang, Jinlan; Cui, Ming; He, Yun; Yu, Hai-lan; Guo, Dan

doi:10.1016/j.jpba.2004.09.009

Cited by 184 publications

(117 citation statements)

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“…Aqueous extract of Danshen was also found to reduce the oxidative stress in endothelial cell [22] and lipid peroxidation in hepatocytes and hepatic stellate cells [23] . DLA can effectively scavenge superoxide anion generated from the xanthin-xanthin oxidase system and protect the myocardial mitochondria from lipid peroxidation [24] . H owe ve r, l i t t l e i s k n ow n a b o u t t h e a c tion of compound Danshen injection on leukocyte-endothelial cell interaction during endotoxin-induced microcirculatory disturbances in vivo.…”

Section: Compound Danshen Injection Improves Endotoxin-induced Microcmentioning

confidence: 99%

“…The analyses were performed using an Agilent series 1100 HPLC system (Agilent, Waldronn, Germany). In order to identify the structures of main constituents in compound Danshen injection, the sample was analyzed by HPLC-MS techniques [24] . Twelve common peaks in compound Danshen injection were designated (Figure 1 …”

Section: Compound Danshen Injection Improves Endotoxin-induced Microcmentioning

confidence: 99%

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Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery

Han

Horie²,

Miura³

et al. 2007

WJG

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stained with Monastral blue B, rolling and adhesion of leukocytes, production of oxygen radical in venular wall, albumin efflux and enhanced mast cell degranulation in vivo , all of which, except for the leukocyte rolling, were attenuated by the treatment with compound Danshen injection. Experiments performed in vitro further revealed that the expression of CD11b/CD18 and the production of oxygen free radical in neutrophils, and the expression of ICAM-1 in HUVECs were increased by exposure to LPS, and they were attenuated by compound Danshen injection. CONCLUSION: These results suggest that compoundDanshen injection is an efficient drug with multi-targeting potential for improving the microcirculatory disturbance. INTRODUCTIONIt is now well-recognized that stimulation with lipopolysaccharide (LPS) leads to a number of responses in microcirculation, such as endothelial cell damage, an increase in rolling, adhesion and emigration of leukocytes in post-capillary venules, enhanced oxygenfree radical production, mast cell degranulation and protein efflux [1][2][3][4][5][6] . Furthermore, it is demonstrated that LPS stimulation evokes an increase in the expression of adhesion molecules, including L-selectin [7] and CD11b/CD18 in leukocytes, E-selectin and intercellular adhesion molecule 1 (ICAM-1) in endothelial cells [7][8][9][10][11][12][13] . In addition, LPS stimulation can cause the release of proinflammatory mediators and vasoactive substances, such Abstract AIM: To investigate the effect of compound Danshen injection on lipopolysaccharide (LPS)-induced rat mesenteric microcirculatory dysfunctions and the underlying possible mechanism by an inverted intravital microscope and high-speed video camera system. METHODS:LPS was continuously infused through the jugular artery of male Wistar rats at the dose of 2 mg/kg per hour. Changes in mesenteric microcirculation, such as diameters of arterioles and venules, velocity of RBCs in venules, leukocyte rolling, adhesion and emigration, free radicals released from post-capillary venules, FITCalbumin leakage and mast cell degranulation, were observed through an inverted intravital microscope assisted with CCD camera and SIT camera. Meanwhile, the expression of adhesion molecules CD11b/CD18 and the production of free radical in neutrophils, and the expression of intercellular adhesion molecule 1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs) were quantified by flow cytometry (FACS) in vitro . RESULTS:The continuous infusion with LPS resulted in a number of responses in microcirculation, including a significant increase in the positive region of venule

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Section: Compound Danshen Injection Improves Endotoxin-induced Microcmentioning

confidence: 99%

Section: Compound Danshen Injection Improves Endotoxin-induced Microcmentioning

confidence: 99%

Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery

Han

Horie²,

Miura³

et al. 2007

WJG

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“…It fragmented at m/z 245 and 211, corresponding with its MS/MS data in the literature [26]. Compound 27 (60.30 min) possessed a deprotonated ion at m/z 431.0974, and was identified as kaempferol-3-Orhamnoside [58]. Its fragment ion at 285 was assigned as the kaempherol moiety.…”

Section: Flavonoidsmentioning

confidence: 80%

“…It fragmented at m/z 301 (quercetin) as its aglycone ion, and fragmented at m/z 609 due to the loss of a rhamnosyl (146 amu), and fragmented at m/z 463 due to another loss of a rhamnosyl. Compound 15 (19.38 min) was identified as quercetin-3,7-O-alpha-L-dirhamnopyranoside with a [M−H] − ion at 593.1513 and a daughter ion at 447 caused by the loss of a rhamnosyl (146 amu) [58]. According to Lin and Harnly [59], the glycosylated quercetin with a monosaccharide at the same position elute in C18 columns with the following order: galactoside, glucoside, xyloside, arabinopyranoside, arabinofuranoside, rhamnoside and glucuronide, and this order was further confirmed by Keinänen and Julkunen-Tiitto [56], 25 [52], and 28 [58] were verified.…”

Section: Flavonoidsmentioning

confidence: 99%

Optimization of Ultrasonic-Assisted Extraction by Response Surface Methodology with Maximal Phenolic Yield and Antioxidant Activity from <em>Acer truncatum</em> Leaves

Yang

Yin

Fan

et al. 2017

Preprint

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This study was designed for the first time to improve phenolic yield and antioxidant activity of ultrasonic-assisted extraction from Acer truncatum leaves (ATL) using response surface methodology, and phenolic composition in ATL extracted under the optimized condition were characterized by UPLC-QTOF-MS/MS. Solvent and extraction time were selected based on preliminary experiments, and a four-factors-three-levels central composite design was conducted to optimize solvent concentration (X1), material-to-liquid ratio (X2), ultrasonic temperature (X3) and power (X4) for an optimal total phenol yield (Y1) and DPPH• antioxidant activity (Y2). The results showed that the optimal combination was ethanol: water (v:v) 66.21%, material-to-liquid ratio 1:15.31 g/mL, ultrasonic temperature 60 °C, power 267.30 W, and time 30 min with three extractions, giving a maximal total phenol yield of 7593.62 mg gallic acid equivalent /100 g d.w. and a maximal DPPH• antioxidant activity of 74241.61 μmol Trolox equivalent/100 g d.w.. Furthermore, 22 phenolics were first identified in ATL extract obtained under the optimized conditions, indicating that gallates, gallotannins, quercetin, myricetin and chlorogenic acid derivatives were the main phenolic composition in ATL. What's more, a gallotannins pathway existing in ATL from gallic acid to penta-O-galloyl-glucoside was interpreted. All these results provided practical information aiming at full utilization of phenolics in ATL, together with fundamental knowledge for further research.

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“…LC-MS/MS methods may overcome the disadvantages that have been reported with the current DM-NCTD analytical methods, including low resolution of HPLC ultraviolet, 52 instability of gas chromatography-MS, 53 high lower limit of quantification, and long chromatography run times. 54 In the current study, an LC-MS/MS method with multiple reaction monitoring was developed and validated for the determination of DM-NCTD in mouse tissues and tumor, using ribavirin as the IS.…”

Section: Validation Of the Analytical Methods In Vivomentioning

confidence: 99%

Folate receptor-targeted liposomes loaded with a diacid metabolite of norcantharidin enhance antitumor potency for H22 hepatocellular carcinoma both in vitro and in vivo

Liu¹,

Liu²,

Wang³

et al. 2016

IJN

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The diacid metabolite of norcantharidin (DM-NCTD) is clinically effective against hepatocellular carcinoma (HCC), but is limited by its short half-life and high incidence of adverse effects at high doses. We developed a DM-NCTD-loaded, folic acid (FA)-modified, polyethylene glycolated (DM-NCTD/FA-PEG) liposome system to enhance the targeting effect and antitumor potency for HCC at a moderate dose based on our previous study. The DM-NCTD/FA-PEG liposome system produced liposomes with regular spherical morphology, with mean particle size approximately 200 nm, and an encapsulation efficiency >80%. MTT cytotoxicity assays demonstrated that the DM-NCTD/FA-PEG liposomes showed significantly stronger cytotoxicity effects on the H22 hepatoma cell line than did PEG liposomes without the FA modification ( P <0.01). We used liquid chromatography–mass spectrometry for determination of DM-NCTD in tissues and tumors, and found it to be sensitive, rapid, and reliable. In addition, the biodistribution study showed that DM-NCTD liposomes improved tumor-targeting efficiency, and DM-NCTD/FA-PEG liposomes exhibited the highest efficiency of the treatments ( P <0.01). Meanwhile, the results indicated that although the active liposome group had an apparently increased tumor-targeting efficiency of DM-NCTD, the risk to the kidney was higher than in the normal liposome group. With regard to in vivo antitumor activity, DM-NCTD/FA-PEG liposomes inhibited tumors in H22 tumor-bearing mice better than either free DM-NCTD or DM-NCTD/PEG liposomes ( P <0.01), and induced considerably more significant cellular apoptosis in the tumors, with no obvious toxicity to the tissues of model mice or the liver tissue of normal mice, as shown by histopathological examination. All these results demonstrate that DM-NCTD-loaded FA-modified liposomes might have potential application for HCC-targeting therapy.

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Chemical fingerprint and metabolic fingerprint analysis of Danshen injection by HPLC–UV and HPLC–MS methods

Cited by 184 publications

References 1 publication

Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery

Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery

Optimization of Ultrasonic-Assisted Extraction by Response Surface Methodology with Maximal Phenolic Yield and Antioxidant Activity from <em>Acer truncatum</em> Leaves

Folate receptor-targeted liposomes loaded with a diacid metabolite of norcantharidin enhance antitumor potency for H22 hepatocellular carcinoma both in vitro and in vivo

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