Chemical derivatization with a polycyclic aromatic hydrocarbon for highly sensitive detection of N-linked glycans using MALDI-TOF MS

Tong, Wei; Han, Huanhuan; Song, Zifeng; Ma, Cheng; Pan, Yiting; Zhang, Yangjun; Qin, Weijie; Qian, Xiaohong

doi:10.1039/c2ay25663k

Cited by 12 publications

(4 citation statements)

References 17 publications

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“…Mass spectrometry (MS) has been proven to be a powerful and effective tool to complete the glycan mapping work. 8,9 However, due to the low abundance and poor ionization efficiency of the available glycan, it is difficult to detect the glycan in complex samples by MS directly. Therefore, it is necessary to separate and purify glycans from complicated samples.…”

mentioning

confidence: 99%

Designed synthesis of MOF-derived magnetic nanoporous carbon materials for selective enrichment of glycans for glycomics analysis

2015

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In this work, magnetic nanoporous carbon (NPC) materials were synthesized by choosing a MOF as a sacrificial template and a carbon precursor. The obtained Co-ZIF-67 materials showed strong magnetic response, high surface area, a uniform size of mesopores and high carbon content. The Co-ZIF-67 materials were successfully applied to glycomics analysis by enriching N-linked glycans in bio-samples with high selectivity and efficiency.

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confidence: 99%

Designed synthesis of MOF-derived magnetic nanoporous carbon materials for selective enrichment of glycans for glycomics analysis

2015

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“…Schiff base formation with ethidium bromide has been used to produce a very sensitive detection system, demonstrated with maltoheptaose and N ‐linked glycans (Tong et al, ). The increase in sensitivity was about sixfold and threefold, respectively, when compared with the signals from 2‐AB‐ or permethylated‐derivatized maltohexaose.…”

Section: Derivativesmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Harvey

2015

Mass Spectrometry Reviews

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This review is the seventh update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2012. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, and fragmentation are covered in the first part of the review and applications to various structural types constitute the remainder. The main groups of compound are oligo- and poly-saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:255-422, 2017.

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“…[5][6][7][8] Therefore, the global N-glycan proling of proteins is crucial not only in elucidating the functions of glycans in biological processes and disease development but also in discovering biomarkers for early diagnosis and therapeutic monitoring. [9][10][11][12] Due to its high sensitivity, accuracy and easy elucidation of spectra, matrix-assisted laser desorption/ionization time of ight mass spectrometry (MALDI-TOF MS) has been a major approach for global glycan identication. 13 The rapid progress in glycomics has resulted in an increasing demand for identication and characterization of N-glycans as efficiently and rapidly as possible.…”

Section: Introductionmentioning

confidence: 99%

A graphene oxide-based immobilized PNGase F reagent for highly efficient N-glycan release and MALDI-TOF MS profiling

et al. 2014

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Protein N-glycosylation plays an important role in a variety of biological processes, and unusual changes in glycan structures are often correlated with various pathological states. Therefore, N-glycan profiling not only plays a vital role in the mechanism study of biological processes but also contributes to the discovery of biomarkers for early disease diagnosis. However, the conventional in-solution release of Nglycans by free endoglycosidases, such as peptide N-glycosidase F (PNGase F), suffers from several drawbacks, including prolonged incubation time, possible incomplete digestion and high cost due to the non-reusability of the enzyme. Herein, we report a novel graphene oxide (GO)-based immobilized PNGase F reagent for fast and highly efficient N-glycan release and glycoform profiling of glycoproteins.To create this reagent, PNGase F was attached to the GO surface via carbodiimide-activated amidation.The ultra-high surface area of GO allows for high loading with PNGase F and significantly facilitates the deglycosylation process. Highly efficient N-glycan release of ribonuclease B and asialofetuin was achieved with this reagent within 2 min. Furthermore, the successful application of our GO-based immobilized PNGase F in the N-glycan release of the human plasma glycoproteome was demonstrated by reproducible N-glycan identification using MALDI-TOF MS.

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Chemical derivatization with a polycyclic aromatic hydrocarbon for highly sensitive detection of N-linked glycans using MALDI-TOF MS

Cited by 12 publications

References 17 publications

Designed synthesis of MOF-derived magnetic nanoporous carbon materials for selective enrichment of glycans for glycomics analysis

Designed synthesis of MOF-derived magnetic nanoporous carbon materials for selective enrichment of glycans for glycomics analysis

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

A graphene oxide-based immobilized PNGase F reagent for highly efficient N-glycan release and MALDI-TOF MS profiling

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