Chemical constituents of ethanol extract of leaves and molluscicidal activity of crude extracts from vitex trifolia linn.

Abstract

S u m m a r y β-sitosterol and two triterpenoids: ursolic acid acetate and platanic acid have been isolated from ethanolic extract of Vitex trifola leaves. β-sitosterol was previously isolated from the leaves, stem and seeds of Vitex trifolia. Ursolic acid acetate has been isolated for the first time in this plant species. Platanic acid has been reported for the first time in Vitex trifolia and even in the family of this plant: Verbenaceae. These compounds were characterized using spectroscopic methods including 1D-1 HNMR, 13 CNMR, ESIMS and 2D-NMR (HSQC, HMBC, COSY) experiments and confirmed by comparison of their NMR data with those from the literature. A preliminary molluscicidal test for ethanol, chloroform and n-hexane extracts of leaves of Vitex trifolia against Biomphalaria alexandrina adult snails showed that ethanol extract of leaves with LC 50 value 26.42 mg/l (27.92 mg/l -24.99 mg/l) was more effective than n-hexane extract with LC 50 value 35.48 mg/l (43.81 mg/l -28.72mg/l) and chloroform extract with LC 50 value 46.77 mg/l (53.59 mg/l -43.81 mg/l) after 24 h exposure.

A Review on the Phytochemistry, Medicinal Properties and Pharmacological Activities of 15 Selected Myanmar Medicinal Plants

“…A series of labdane-type diterpenoids, vitextrifolins A–G ( 145 – 151 ), together with rotundifuran, vitetrifolin H, vitexilactone, vitexilactone B, deacetylvitexilactone, viteagnusin I, previtexilactone and negundol were isolated from the fruits of V. trifolia [112]. Additionally, an iridoid agnuside ( 152 ) was found in the leaves of the same species [113].…”

“…Negundoside ( 153 ), agnuside ( 152 ) and 6- p -hydroxybenzoyl mussaenosidic acid ( 154 ) isolated from the aerial parts of V. trifolia showed significant antioxidant activity with IC 50 values of 9.96, 9.81 and 10.31 μg, respectively in DPPH and 16.25, 12.90 and 13.51 μg in NO radical scavenging assays [114]. The ethanol extract of the leaves of V. trifolia displayed free radical scavenging activity [113]. The methyl- p -hydroxybenzoate ( 155 ), which is reported from V. trifolia possessed potential mosquito larvicidal activity with the LC 50 values of 5.77 ppm against Culex quinquefasciatus and 4.74 ppm against Aedes aegypti [115].…”

ED50 from anti-inflammatory properties of Vitex trifolia L. ethanol extract

“…There was no further effort to determine the type of spot. Jangwani et al [20] reported that they had isolated β-sitosterol and two triterpenoids: Ursolic acid acetate and platanic acid from ethanolic extract of V. trifola leaves. …”

Traditional Medicinal Uses, Phytochemistry, Biological Properties, and Health Applications of Vitex sp.

“…With respect to different sorts of Vitex species and depending on the country, they are also known by a variety of names, such as Nirgundi, Sambhalu, Gattiler, Arabian Lilac, Manjingzi, and Legundi [ 5 , 6 , 7 , 8 ]. Despite the different names, Vitex has been historically known and utilized by prehistoric civilization for various purposes, including treatment for reproductive disorders, gastrointestinal conditions, inflammatory diseases, and the ability to subdue several symptoms of psychiatric illnesses [ 9 , 10 , 11 , 12 ]. Nowadays, many studies are being conducted to investigate the biological properties of Vitex genus, as they are believed to contain a pool of metabolites with potential complementary therapeutic actions.…”