Chemical constituents and biological activities against Tribolium castaneum (Herbst) of the essential oil from Citrus wilsonii leaves

Abstract

The essential oil obtained from Citrus wilsonii Tanaka leaves by hydrodistillation was investigated by GC and GC-MS. The main components of the essential oil were identified to be citronellol (16.94 %), neryl acetate (10.35 %), γ-terpinene (9.85 %), citronellal (9.36 %) and β-pinene (6.72 %). These four compounds, predicted with a bioactivity-test to be the active constituents, were isolated and identified. It was found that the essential oil of C. wilsonii leaves and the isolated compounds possessed fumigant and contact toxicity against Tribolium castaneum adults. The essential oil and γ-terpinene showed strong fumigant toxicity against T. castaneum (LC 50 = 8.18 and 4.09 mg L -1 , respectively). The repellency of the crude oil and the active compounds was also determined. Citronellol, neryl acetate and β-pinene were strongly repellent (100, 86 and 92 %, respectively, at 78.63 nL cm -2 , after 2 h treatment) against T. castaneum. The essential oil and citronellol exhibited the same level of repellency compared with the positive control, N, N,. The results indicate that the essential oil of C. wilsonii leaves and its active compounds had the potential to be developed as natural fumigants, insecticides and repellents for the control of T. castaneum.

Contact and Repellent Activities of the Essential Oil from Juniperus formosana against Two Stored Product Insects

“…Estragole was also found to have contact and repellent activities against T. castaneum , L. bostrychophila [38], Lasioderma serricorne , S. oryzae , Callosobruchus chinensis [41] and S. zeamais [42,43]. Additionally, γ-terpinene was found to exhibit activity against T. castaneum [44] and show high toxicity against Anopheles sinensis , A. aegypti , Aedes albopictus and Culex pipiens pallens mosquito larvae [45]. Moreover, germacrene D is known to have a strong effect on insect behavior [46], and α-terpineol exhibited fumigant, contact and repellent bioactivities against L. serricorne [47].…”

Chemical Variability of Peel and Leaf Essential Oils in the Citrus Subgenus Papeda (Swingle) and Few Relatives

“…The leaf oil composition of C. wilsonii was characterized by the association of γterpinene (19.5%), geranial (15.6%), neral (11.6%), and β-pinene (9.7%). Previously reported chemical compositions were drastically different: (i) γ-terpinene (12.9%), thymol (9.8%), β-pinene (8%), (E)-β-ocimene (6.9%), and p-cymene (4.5%) [19]; (ii) linalool (38.2%), γterpinene (25.4%), p-cymene (14.6%), neryl acetate (12.5%), β-pinene (9.8%), and nerol (5.8%) [19]; and iii) citronellol (16.9%), followed by neryl acetate (10.4%), γ-terpinene (9.9%), citronellal (9.4%), and β-pinene (6.7%) [44]. Lota et al [37] described a similar chemical profile with quantitative variations: γ-terpinene (36.1%), geranial (3.4%), neral (2.3%), and β-pinene (14%).…”

Bioactivity of Essential Oil from <i>Artemisia stolonifera</i> (Maxim.) Komar. and Its Main Compounds against Two Stored-Product Insects

“…Besides insecticidal and repellent activity, essential oils from different plant sources have also exhibited several biological activities, including antimicrobial 18 and antifungal 19 , nematicidal 20 , larvicidal 21 and acaricidal 22 . Actually, this vast arsenal of bioactive compounds has attracted attention of researchers in recent years.…”