Chemical Compositions, Somatic Embryogenesis, and Somaclonal Variation in Cumin

Bahmankar, Moslem; Mortazavian, Seyed Mohammad Mahdi; Tohidfar, Masoud; Noori, Seyed Ahmad Sadat; Izadi-Darbandi, Ali; Corrado, Giandomenico; Rao, Rosa

doi:10.1155/2017/7283806

Cited by 26 publications

(20 citation statements)

References 63 publications

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“…All polymorphic bands among parent donor and its somaclones were occurred in parental bands which were absence in some somaclones and/or new non-parental bands which were generated only in some somaclones. This result is in line with determination of genetic polymorphism of somaclones in cumin, biochemical markers (Gupta, 2013), RAPD (Bahmankar et al, 2019;Bahraminejad et al, 2012;Soniya et al, 2001), SRAP (Bhatt et al, 2017).…”

Section: Discussionsupporting

confidence: 74%

Establishment of Efficient In vitro Culture Protocol and Screening of Soma clonal Variation Among Regenerated Plants in Cumin (Cuminum cyminum L.)

Hussien

Taghian²,

El-Aref³

et al. 2022

AJAS

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Genetic diversity among in vitro plant regeneration in cumin has been examined by molecular markers analysis (ISSR and SRAP markers). Two landraces of cumin named Egyptian landraces (EGY genotype) and Indian landraces (IND genotype) were used as donor parents, three types of explants (hypocotyl, cotyledon and root) and four MS tissue culture media with different concentrations of growth regulators (auxin and cytokinin) were used to study the impact of genotype, type of explants and growth regulators on callus formation and plant regeneration in cumin. Significant differences among two cumin landraces were observed for regeneration rate and number of shoots per explant. These differences were depending on genotype, explant type and concentration of growth regulators. The best regeneration medium (MS with 0.5 mg/L 2,4-D) used for establishment of regenerated plants. Donor parent, Egyptian landraces (EGY) and Indian landraces (IND) and its regenerated plants on regeneration medium were selected and subjected to somaclonal variation analysis using molecular markers. ISSR (inter-simple-sequence-repeat) and SRAP (sequence-relatedamplified-polymorphism) markers were used to detect the genetic variations between two cumin landraces. Subsequently, primers which exhibited high polymorphism between donor parents were used to analysis of somaclonal variation between each donor parent and its regenerated plants (somaclones), as well as among somaclones. These markers revealed polymorphism showing clear different DNA fragment patterns in all somaclones, which were eminent in their differences from parents.

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Section: Discussionsupporting

confidence: 74%

Establishment of Efficient In vitro Culture Protocol and Screening of Soma clonal Variation Among Regenerated Plants in Cumin (Cuminum cyminum L.)

Hussien

Taghian²,

El-Aref³

et al. 2022

AJAS

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“…It can modulate redox signaling and, its overexpression in tomato could increase ROS and NO scavenging efficiency (60). Competence for SE induction was shown to be positively linked to the amount of anti-oxidant secondary plant compounds and enzymes (18,26,(61)(62)(63)(64)(65). It is relevant to mention that high levels of NO can counteract SE induction, highly lightening the importance of balanced ROS/ RNS homeodynamics in cells.…”

Section: Somatic Embryogenesis (Se) -An Experimental Tool To Identify Markers For Early Reprogramming and Resiliencementioning

confidence: 99%

From Plant Survival Under Severe Stress to Anti-Viral Human Defense – A Perspective That Calls for Common Efforts

et al. 2021

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Reprogramming of primary virus-infected cells is the critical step that turns viral attacks harmful to humans by initiating super-spreading at cell, organism and population levels. To develop early anti-viral therapies and proactive administration, it is important to understand the very first steps of this process. Plant somatic embryogenesis (SE) is the earliest and most studied model for de novo programming upon severe stress that, in contrast to virus attacks, promotes individual cell and organism survival. We argued that transcript level profiles of target genes established from in vitro SE induction as reference compared to virus-induced profiles can identify differential virus traits that link to harmful reprogramming. To validate this hypothesis, we selected a standard set of genes named ‘ReprogVirus’. This approach was recently applied and published. It resulted in identifying ‘CoV-MAC-TED’, a complex trait that is promising to support combating SARS-CoV-2-induced cell reprogramming in primary infected nose and mouth cells. In this perspective, we aim to explain the rationale of our scientific approach. We are highlighting relevant background knowledge on SE, emphasize the role of alternative oxidase in plant reprogramming and resilience as a learning tool for designing human virus-defense strategies and, present the list of selected genes. As an outlook, we announce wider data collection in a ‘ReprogVirus Platform’ to support anti-viral strategy design through common efforts.

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“…Proline had no visible impact on the shoot proliferation of nodal explant of R. canina. This amino acid was previously reported to be effective in somatic embryogenesis in certain plant species [44,45]. Although proline at the maximum concentration (2 g/l) improved shoot length and leaf number, it failed to stimulate shoot regeneration in the nodal explant of R. canina and thus it is not suggested to be used for direct in vitro regeneration of this plant.…”

Section: Discussionmentioning

confidence: 99%

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Samiei

Pahnehkolayi

Tehranifar

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Rosa canina is one of the most popular rose species which is widely used as the rootstock for the propagation of rose cultivars. The purpose of the present study is to improve the in vitro propagation efficiency of this valuable plant species using various growth stimulants in a proliferation medium. In this study, in vitro-derived axillary buds of R. canina were inoculated in Vander Salm (VS) medium supplemented with varying levels of organic or inorganic elicitors including casein hydrolysate (200, 400, and 600 mg/l), glutamic acid (2, 4, 8, and 12 mg/l), proline (500, 1000, 1500, and 2000 mg/l), and silver nitrate (25, 50, 75, and 100 mg/l), separately. Benzyl amino purine (BAP) as well as naphthalin acetic acid (NAA) were added to all media at a constant rate to promote shoot proliferation. Results The results indicated that the supplementation of casein hydrolysate to the VS medium markedly stimulated shoot regeneration by 173% in comparison to control. Shoot proliferation was also positively influenced by glutamic acid at all levels, however, at a lesser extent compared to casein hydrolysate. Silver nitrate at 100 mg/l induced the longest shoots (2.52 ± 0.248 cm) and maximum leaf number (8.90 ± 0.276) among all treatments. Although it did not encourage efficient shoot regeneration, the highest quality shoots with maximum growth vigor were observed in this treatment. Conclusion In this study, the promising role of casein hydrolysate in combination with plant growth regulators has been emphasized for the improved efficiency of R. canina regeneration protocol. Moreover, the addition of silver nitrate to the culture medium seems vital for enhancing the quality of regenerated shoots. The results of this study could be beneficial either for the further pharmaceutical or biochemical investigations of R. canina or commercial purposes for mass propagation of this specimen.

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Chemical Compositions, Somatic Embryogenesis, and Somaclonal Variation in Cumin

Cited by 26 publications

References 63 publications

Establishment of Efficient In vitro Culture Protocol and Screening of Soma clonal Variation Among Regenerated Plants in Cumin (Cuminum cyminum L.)

Establishment of Efficient In vitro Culture Protocol and Screening of Soma clonal Variation Among Regenerated Plants in Cumin (Cuminum cyminum L.)

From Plant Survival Under Severe Stress to Anti-Viral Human Defense – A Perspective That Calls for Common Efforts

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

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