Chemical Compositions, Chromatographic Fingerprints and Antioxidant Activities of Andrographis Herba

Abstract

This paper describes the development of an HPLC-UV-MS method for quantitative determination of andrographolide and dehydroandrographolide in Andrographis Herba and establishment of its chromatographic fingerprint. The method was validated for linearity, limit of detection and quantification, inter-and intra-day precisions, repeatability, stability and recovery. All the validation results of quantitative determination and fingerprinting methods were satisfactory. The developed method was then applied to assay the contents of andrographolide and dehydroandrographolide and to acquire the fingerprints of all the collected Andrographis Herba samples. Furthermore, similarity analysis and principal component analysis were used to reveal the similarities and differences between the samples on the basis of the characteristic peaks. More importantly, the DPPH free radical-scavenging and ferric reducing capacities of the Andrographis Herba samples were assayed. By bivariate correlation analysis, we found that six compounds are positively correlated to DPPH free radical scavenging and ferric reducing capacities, and four compounds are negatively correlated to DPPH free radical scavenging and ferric reducing capacities.

Antioxidant activities and liquid chromatography with electrospray ionization tandem mass spectrometry characterization and quantification of the polyphenolic contents ofRumex nervosusVahl leaves and stems

“…In the present study, we extend our investigation to the stems and leaves of R. nervosus to characterize and quantify their polyphenolic components using LC–ESI‐MS/MS along with various non‐enzymatic in vitro antioxidant activities of separate tissue extracts. Because of its high sensitivity and selectivity, LC–MS/MS is one of the most extensively used techniques in analysis of phytochemicals as well as QC .…”

Andrographolide, A Natural Antioxidant: An Update

“…One study used the HPLC-UV-MS method and the DPPH test, determining the free radical scavenging activities in the different extracts. The radical scavenging activity of all samples was lower than the positive control, BHT [40]. A study showed that an aqueous extract of Andrographis paniculata exhibited greater antioxidant activity than an ethanol extract.…”

“…SOD, CAT, and GSH activities decreased in the gastric tissue of rats treated with indomethacin. Oral administration of andrographolide sodium bisulfate (40,80, and 160 mg/kg) 7 days before treatment with indomethacin reduced oxidative stress by restoring SOD, CAT, and GSH activities [72]. Mice treated with arsenic have decreased SOD and CAT activities.…”

Fingerprint profiles of flavonoid compounds from different Psidium guajava leaves and their antioxidant activities

“…DPPH radical scavenging activity was determined using a method previously described by Hammi et al with slight modifications [23]. The reaction system contained 400 μL of a DPPH methanol solution (100 μM) and 50 μL dilutions of different concentrations of flavonoid extracts (10,20,30,50,80, and 100 μg/mL). The reaction was performed at 25 • C for 30 min.…”