In this study, the composition and antioxidant activity of Pistacia lentiscus L. leaf essential oil (EO) from four Tunisian localities were investigated. The EO yields (%, w/w) of P. lentiscus leaf were 0.009% in Oued El Bir, 0.02% in Jebel Mansour, 0.007% in Siliana and 0.01% in Tabarka. The main compounds of Oued El Bir EO were terpinene-4-ol (41.24%) and aterpineol (7.31%), whereas those of Jebel Mansour were a-pinene (9.48%), limonene (19.11%) and a-phellandrene (3.20%). In Siliana samples, terpinene-4-ol (23.32%), b-caryophyllene (22.62%) and a-terpineol (7.12%) were the main compounds. For P. lentiscus L. from Tabarka, a-terpineol (9.79%) and b-caryophyllene (38.33%) were the major constituents. Three chemotypes of P. lentiscus EO were identified for the first time in Tunisia. P. lentiscus EOs were also screened for their antioxidant activities. The 1,1-diphenyl-2-picrylhydrazyl assay gives an IC 50 range value of 60-110 mg/mL for all the samples studied. EOs from different Tunisian localities showed lower b-carotene bleaching activity than butylated hydroxytoluene and butylated hydroxyanisol. P. lentiscus EO presents a very low capacity to chelate ferrous irons (IC 50 ¼ 80.8-104 mg/mL) and no metal chelating activity was recorded.