Chemical composition of Hypogymnia physodes lichen and biological activities of some its major metabolites

Ranković, Branislav; Kosanić, Marijana; Manojlović, N.; Rančić, Aleksandar; Stanojković, Tatjana

doi:10.1007/s00044-013-0644-y

Cited by 61 publications

(58 citation statements)

References 29 publications

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“…To the best of our knowledge, only the cytotoxic effects of methanolic extract from H. physodes has been studied in MCF-7 and MDA-MB-231 cell lines (IC 50 ¼ 50 and 44 lg/mL, respectively) (Ari et al 2014). The cytotoxic activity of physodic acid isolated from H. physodes in FemX (IC 50 ¼ 19.52 lg/mL) and LS174 (IC 50 ¼ 17.89 lg/mL) has also been studied (Ranković et al 2014). Pavlović et al (2013) reported the biological activity of physodic, physodalic, 3-hydroxyphysodic and isophysodic acids isolated from H. physodes in rat Values are expressed as mean n ¼ 6 replications; measured after 72 h of exposure the cells to the test compounds.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic activity of physodic acid and acetone extract fromHypogymnia physodesagainst breast cancer cell lines

Studzińska-Sroka

Piotrowska

Kucińska

et al. 2016

Pharmaceutical Biology

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Context: Lichens produce specific secondary metabolites with different biological activity. Objective: This study investigated the cytotoxic effects of physodic acid, in addition to the total phenolic content and cytotoxic and antioxidant activity of acetone extract from Hypogymnia physodes (L.) Nyl. (Parmeliaceae). Materials and methods: Cytotoxicity of physodic acid (0.1-100 lM) was assessed in MDA-MB-231, MCF-7 and T-47D breast cancer cell lines and a nontumorigenic MCF-10A cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, neutral red uptake and crystal violet assays during 72 h of incubation. An MTT assay was also used to assess the cytotoxic effects of the acetone extract (0.1-100 lg/mL) in the MDA-MB-231, MCF-7, T-47D breast cancer cell lines after 72 h. The total phenolic content of the acetone extract, expressed as the gallic acid equivalent, was investigated using Folin-Ciocalteu reagent. The antioxidant activity of the extract was assessed by 2,2-diphenyl-1-picrylhydrazyl and ferric-reducing antioxidant power assays. Results: The cytotoxic activity of physodic acid appeared to be strong in the tumorigenic cell lines (IC 50 46.0-93.9 lM). The compound was inactive against the nontumorigenic MCF-10A cell line (IC 50 >100 lM). The acetone extract showed cytotoxicity in the breast cancer cell lines (IC 50 46.2-110.4 lg/mL).

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Section: Discussionmentioning

confidence: 99%

Cytotoxic activity of physodic acid and acetone extract fromHypogymnia physodesagainst breast cancer cell lines

Studzińska-Sroka

Piotrowska

Kucińska

et al. 2016

Pharmaceutical Biology

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“…Both tested extracts of C. aculeata showed higher scavenging activity compared to the methanol extracts of Parmelia sulcata, Flavoparmelia caperata, Evernia prunastri and Cladonia foliacea, while the methanol extract of C. aculeata showed the lower scavenging activity than the methanol extract of Hypogymnia physodes [46]. Significant antioxidant activity of extract of lichen Hypogymnia physodes arises from his lichen compounds (depsides, depsidones and usnic acid), which demonstrated strong antioxidant effects [24]. Phenol compounds are very important plant constituents because their hydroxyl groups contributed to their scavenging ability [47].…”

Section: Discussionmentioning

confidence: 92%

“…Secondary metabolites that have been identified from various species of lichen extracts manifested high antioxidant activity [23,24,25].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant activity of lichen Cetraria aculeatas

Tomović¹,

Rančić²,

Vasiljević³

et al. 2015

Praxis Med

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The aim of the present study is to investigate the antioxidant properties of the lichen Cetraria aculeata. Antioxidant activity of the methanol and ethyl acetate extracts of lichen was tested by different methods including determination of total phenolics content, determination of total antioxidant capacity, DPPH free radical scavenging activity, inhibitory activity towards lipid peroxidation, ferrous ion chelating ability and hydroxyl radical scavenging activity. The extracts of the lichen C. aculeata showed significant antioxidant activity. The methanol extract showed higher values for total phenolics and total antioxidant capacity compared to the ethyl acetate extract, while the ethyl acetate extract demonstrated better results for DPPH radical scavenging, inhibitory activity towards lipid peroxidation, chelating ability and hydroxyl radical scavenging than the methanol extract. This is the first report of the antioxidant properties of Cetraria aculeata growing in Serbia. The results of antioxidant activity indicate the application of this lichen as source of natural antioxidants that could be used as a possible food supplement, in the pharmaceutical industry and in the treatment of various diseases.Keywords: ROS, antioxidant activity, lichen, Cetraria aculeata. INTRODUCTIONActive oxygen exists in different forms, such as superoxide anion radicals (O2• −), hydrogen peroxide (H 2 O 2 ), hydroxyl radicals (OH•) and singlet oxygen ( 1 O2). These forms of oxygen are highly reactive intermediates, and have a collective name of reactive oxygen species (ROS) [1,2,3,4,5]. ROS are free radicals very important for living organisms, in which are formed in different ways. In normal aerobic respiration, stimulated polymorphonuclear leukocytes and macrophages, and peroxisomes appear to be the main endogenous sources of most oxidants produced by cells. However, despite the fact that ROS are necessary for cell function, in high concentrations leads to oxidative stress and to the development a large number of diseases such as arthritis, carcinogenesis, aging. In addition to endogenous sources of free radicals, a major contribution of the accumulation of free radicals in cells are provided by exogenous sources such as ionizing radiation, tobacco smoke, certain pollutants, organic solvents and pesticides [4,6,7,8,9,10]. The above mentioned diseases and accelerated aging are the consequence of oxidative tissue damage by free radicals because of unbalanced mechanisms of antioxidant protection under the influence of endogenous and exogenous factors [11]. ROS attack unsaturated fatty acids in membrane proteins, causing lipid peroxidation, and the result is damage to membrane proteins [12]. This leads to reduced permeability of membranes, receptors and enzyme activity, and reduced activation of cells. Free radicals attack the DNA, while leading to DNA damage, resulting in mutations that cause cancer. Therefore, the prevention of many diseases are important antioxidant defense systems, including food, drugs and antioxidant enzymes [1...

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“…As a result of examination of DPPH and superoxide anion capture and reducing power activities of PHA, it was detected that PHA metabolite had high antioxidant capacity (Kosanić & Ranković 2011;Kosanić et al 2013;Ranković et al 2014). Furthermore, it was reported that PHA that showed antimutagenic effect inhibiting the formation of reactive metabolites (Osawa et al 1991) possessed antimicrobial activity (Yilmaz et al 2005;Kosanić & Ranković 2011;Kosanić et al 2013;Ranković et al 2014) if it was used at certain concentrations. In recent years, cytotoxic effects of PHA tested toxicity on microorganisms were evaluated on different cells.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, cytotoxic effects of PHA tested toxicity on microorganisms were evaluated on different cells. Proliferation inhibitory activities on rat thymocytes (Pavlovic et al 2013), human melanoma and colon cancer cells (Kosanić et al 2013;Ranković et al 2014) and cervical cancer cells (Stojanović et al 2014) of PHA metabolite were investigated.…”

Section: Discussionmentioning

confidence: 99%

In vitroantitumor activities of the lichen compounds olivetoric, physodic and psoromic acid in rat neuron and glioblastoma cells

Emsen

Aslan

Toğar

et al. 2015

Pharmaceutical Biology

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Context Since methods utilised in the treatment of glioblastoma multiforme (GBM) are inadequate and have too many side effects, usage of herbal products in the treatment process comes into prominence. Lichens are symbiotic organisms used for medicinal purposes for many years. There are various anticancer treatments about components of two lichen species used in the present study. Objective Antitumor potential of three lichen secondary metabolites including olivetoric acid (OLA) and physodic acid (PHA) isolated from Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) and psoromic acid (PSA) isolated from Rhizoplaca melanophthalma (DC.) Leuckert (Lecanoraceae) were investigated on human U87MG-GBM cell lines and primary rat cerebral cortex (PRCC) cells for the first time. Materials and methods PRCC cells used as healthy brain cells were obtained from SpragueDawley rats. The treatments were carried out on the cells cultured for 48 h. Cytotoxic effects of different concentrations (2.5, 5, 10, 20 and 40 mg/L) of metabolites on the cells were determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) analyses. Total antioxidant capacity (TAC) and total oxidant status (TOS) parameters were used for assessing oxidative alterations. Oxidative DNA damage potentials of metabolites were investigated via evaluating 8-hydroxy-2 0 -deoxyguanosine (8-OH-dG) levels. Results Median inhibitory concentration (IC 50 ) values of OLA, PHA and PSA were 125.71, 698.19 and 79.40 mg/L for PRCC cells and 17.55, 410.72 and 56.22 mg/L for U87MG cells, respectively. It was revealed that cytotoxic effects of these metabolites showed positive correlation with concentration, LDH activity and oxidative DNA damage. Discussion and conclusion The present findings obtained in this study revealed that primarily OLA and then PSA had high potential for use in the treatment of GBM. ARTICLE HISTORY

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Chemical composition of Hypogymnia physodes lichen and biological activities of some its major metabolites

Cited by 61 publications

References 29 publications

Cytotoxic activity of physodic acid and acetone extract fromHypogymnia physodesagainst breast cancer cell lines

Cytotoxic activity of physodic acid and acetone extract fromHypogymnia physodesagainst breast cancer cell lines

Antioxidant activity of lichen Cetraria aculeatas

In vitroantitumor activities of the lichen compounds olivetoric, physodic and psoromic acid in rat neuron and glioblastoma cells

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