Chemical composition, antioxidant and anti-inflammatory activities of the essential oils from male and female specimens of Baccharis punctulata (Asteraceae)

Abstract

Baccharis punctulata (Asteraceae), popularly known as "Chíllka saru saru" in Bolivia, has been used by rural communities in Bustillo Province of the Potosi Department for treatment of asthma, luxations and contusions. Aim of the study: To analyze the chemical composition of the essential oils obtained from leaves of female (BPF) and male (BPM) specimens and evaluate their anti-inflammatory and antioxidant properties. Material and methods: Chemical composition analyses of Baccharis punctulata essential oils isolated by hidrodistillation from leaves of male and female specimens were performed by GC-FID-MS. The in vivo anti-inflammatory activity was evaluated using the model of TPA (12-O-tetradecanoylphorbol-13-acetate) induced ear edema, and the polymorphonuclear cell migration was evaluated by mieloperoxidase (MPO) and analyzed histologically. To measure the reactive oxygen species (ROS) in the inflamed tissue, the DCFH-DA fluorescent probe was used. The chemical in vitro antioxidant activity of essential oils was determined using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical assay. Results: The chemical analysis showed high proportion of sesquiterpenes in the volatiles samples obtained from BPM, such as δ-elemene (14.29%), germacrene D (11.29%) and bicyclogermacrene (10.90%), and in the sample from BPF, bicyclogermacrene (42.44%), germacrene D (21.18%) and β-caryophyllene (14.06%). A statistical difference (p < 0.05) on chemical composition between both essential oils was observed. Topical administration of both BPM and BPF essential oils was able to inhibit the formation of TPA-induced edema in the treated groups. Isolated administration of TPA promoted an increase in MPO enzyme activity, and inhibition of the increase of MPO activity was observed when animals were treated with BFP at concentrations of 0.1 mg/ear (13.69 ± 0.20%), 0.3 mg/ear (22.35 ± 0.11%), and 1.0 mg/ear (44.98 ± 0.27%). Topical treatment with BPM was able to inhibit MPO activity at 22.40 ± 0.29% (0.1 mg/ear), 36.49 ± 0.07% (0.3 mg/ear) and 52.19 ± 0.28% (1.0 mg/ear). The positive control of dexamethasone (DEXA, 0.1 mg/ear) was able to revert the increase in the enzymatic activity of MPO caused by TPA (65.16%). Histological analysis showed that topical application of TPA promoted intense cellular infiltration. This inflammatory parameter was reduced with the topical application of the BPF and BPM oil samples as well as with DEXA. The results observed in the ROS and DPPH tests suggest that both samples were able to reduce the inflammatory cells influx and have in vitro antioxidant properties, respectively. Conclusions: This study presents, for the first time, the chemical composition of the essential oils obtained from

Influence of Environmental Factors on the Genetic and Chemical Diversity of Brickellia veronicifolia Populations Growing in Fragmented Shrublands from Mexico

“…Intriguingly, the chemical profile reported in our work remained constant in all the studied populations of B. veronicifolia at the expense of environmental conditions and genetic constitution. The major sesquiterpenoids reported in our work have also been found in other Asteraceae plants such as Centaurea tuberosa [47] and Eremanthus erythropappus [48].…”

Antibacterial, antioxidant and enzyme inhibition activity capacities of Doronicum macrolepis (FREYN&SINT): An endemic plant from Turkey

“…In literature, no study examining the amylase, glucosidase, and cholinesterase inhibition activities of Doronicum species was found. However, the enzyme inhibition activity of many plants from the Asteraceae, which includes also Doronicum , was investigated before (Saoud et al, 2019, Uysal et al, 2018, Ascari et al, 2019, Zengin et al, 2018, Saleem et al, 2019a, Saleem et al, 2019b).…”

Effect of Terpenoids and Flavonoids Isolated from Baccharis conferta Kunth on TPA-Induced Ear Edema in Mice

“…In addition, it promotes the activation of the MAPKs pathway, which induces the release of pro-inflammatory cytokines such as interleukin (IL)-1, tumor necrosis factor (TNF)-α, IL-8 and macrophage inflammatory protein (MIP)-1β. Adhesion molecules and enzymes are activated, resulting in the formation of edema and the migration of leukocytes into the dermis [16]. The anti-inflammatory activities of the BcH, BcD and BcM extracts of B. conferta using the TPA-induced ear edema model in mice are shown in Table 1.…”

“…Neoclerodanes diterpenes are characteristic constituents from this genus and possess a great anti-inflammatory effect in acute and chronic inflammatory conditions. Twenty-four diterpenoids have been reported, and 16 new terpenoids have been isolated, including seven neoclerodanes [1][2][3][4][5][6][7]. Hautriwaic acid, a neoclerodane diterpene, showed anti-inflammatory activity on two murine models: ear edema induced with 12-O-tretradecanoylphorbol-13-acetate (TPA) and monoarthritis induced with kaolin/carrageenan [8,9].…”