Chemical composition, antimicrobial properties and toxicity evaluation of the essential oil of Cupressus lusitanica Mill. leaves from Cameroon

Teke, Gerald Ngo; Elisée, Kemadjou Nana; Roger, Kuiate Jules

doi:10.1186/1472-6882-13-130

Cited by 33 publications

(35 citation statements)

References 27 publications

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“…The morphology, stability and size of AgNPs formed depend on the method of its preparation, nature of the solvent used, temperature and strength of the reducing agent. On this study, AgNPs were biosynthesized using aqueous Cupressus macrocarpa extract because it contains reducing stabilizing agents [35,36]. In the present study formation of AgNPs was indicated by a change in color from pale yellow to dark brown (Figure 1-I-A).…”

Section: Biosynthesis Of Agnpsmentioning

confidence: 64%

Biosynthesis and application of silver nano-biotics for enhancing the antimicrobial activity of glass-ionomer cement against studied microorganisms and its effect on compressive strength

Enan

Ashour

Basha

et al. 2020

Preprint

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Background. The development of dental caries is associated with various microorganisms and secondary caries formation is the main cause of restorations failure. The advise for restorative dental materials that have antimicrobial properties has stimulated the introduction of materials containing different antibacterial agents.Objectives: Present study has been designed to synthesize silver nanoparticles (AgNPs) and incorporate AgNPs and amoxicillin into glass ionomer cement (GIC) to synergize its effect on oral microbes. The effect of the added antimicrobial agents on compressive strength (CS) of GIC was also evaluated.Material and methods: Biosynthesis of AgNPs were done using Cupressus macrocarpa extract and AgNPs were charectatrized. A total of 120 disc-shaped specimens were prepared and classified into 4 main groups where Group A include conventional GIC, Groups B and C include GIC with AgNPs or Amoxicillin, respectively, while Group D included GIC with both AgNPs and Amoxicillin. Each group was tested for the antimicrobial activity against both Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus). The distribution of biofilm was examined via scanning electron microscope. The CS of the tested material was measured using a Material Test System(MTS).Results: UV-Visible spectrum showed a peak of 429 nm. TEM, XRD pattern and FTIR analysis confirmed the formation of AgNPs with spherical to oblong polydispersed particles of diameter in the range of 13.5-25.8 nm. The maximum inhibitory zone was recorded for group D against both tested bacteria with a mean of 29 mm at first 24-hour period to 15 mm at three weeks and showed antimicrobial rate 92.2% and 92.56%, against both strains, respectively. Additionally, group D disintegrated the structure of S. aureus biofilm and even kill bacteria in the biofilms. Addition of AgNPs and Amoxicillin caused an insignificant effect on CS of GIC.Conclusion: TheAgNPs showed a synergistic effect in combination with amoxicillin and GIC dental restorative material against studied microorganisms. The agents can be safely added with minimal effect on mechanical properties of the original cement.

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Section: Biosynthesis Of Agnpsmentioning

confidence: 64%

Biosynthesis and application of silver nano-biotics for enhancing the antimicrobial activity of glass-ionomer cement against studied microorganisms and its effect on compressive strength

Enan

Ashour

Basha

et al. 2020

Preprint

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“…Thus, T. articulata EO falls in Class 5 (a substance with LD 50 higher than 5000 mg/kg), hence the EO can be considered with low toxicity 25 . Similar results were found by Teke et al., (2013), 48 who did not observe any mortality after administration of the EO from Cupressus lusitanica an other member of the Cupressaceae family.…”

Section: Resultsmentioning

confidence: 97%

Chemical composition, acute toxicity, antioxidant and anti-inflammatory activities of Moroccan Tetraclinis articulata L.

Jemli¹,

Kamal²,

Marmouzi³

et al. 2017

Journal of Traditional and Complementary Medicine

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Hydro-distilled essential oil (EO) from the leaves of the western Mediterranean and Moroccan endemic plant Tetraclinis articulata was analyzed by GC/MS and examined for its acute toxicity on mice, in order to establish the safe doses. Furthermore, the anti-Inflammatory activity was evaluated based on carrageenan and trauma induced rats paw edema and the antioxidant potential has been investigated using different methods including DPPH radical-scavenging assay, Trolox equivalent antioxidant capacity (TEAC) and Ferric-reducing antioxidant power assay (FRAP). The major identified compounds in GC/MS analysis were bornyl acetate (26.81%), camphor (22.40%) and α-pinene (7.16%), with 25 other minor constituents. No mortalities in acute toxicity were observed, indicating that the LD50 of T. articulata essential oil is highest than 5 g/kg. In the anti-inflammatory test based on chemical and mechanical induced trauma, the EO demonstrated an effective reduce swelling by 64.71 ± 9.38% and 69.09 ± 6.02% respectively obtained 6 h after administration at the dose of 200 mg/kg when compared to the control groups. Moreover in the antioxidant testing battery, T. articulata essential oil showed a promising scavenging effect measured by DPPH, TEAC and ferric-reducing power assays with IC50 values of 12.05 ± 0.24 mg/mL, 8.90 ± 0.17 mg/mL and 0.15 ± 0.01 mg/mL respectively. These results suggest that, the EO from the leaves of T. articulata constitutes a valuable source of anti-inflammatory and antioxidant metabolites. These findings argue for the possible integration of this oil in pharmaceutical, cosmetic and food industries.

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“…The methanolic extract of Echinops robustus was tested using agar disc diffusion technique for determination of the growth inhibition zones and also broth macro-dilution method was used to determine the MIC and MBC [10].…”

Section: Antimicrobial Assaymentioning

confidence: 99%

Chemical composition and antibacterial activity of methanolic extract from Echinops robustus on typical food-borne pathogens

2020

Lett Appl NanoBioSci

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This study was conducted to evaluate the chemical composition and antibacterial activity of methanolic extract from Echinops robustus on typical food-borne pathogens. Chemical composition of the extract analyzed by gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis of the extract revealed 54 compounds in which Butanal (2.222%), 4-Heptanone (1.717%) and Palmitic acid (4.799%) were the major constituents. The agar disk diffusion method was used to study the antibacterial activity of Echinops robustus methanolic extract against 5 bacterial strains. The extract of Echinops robustus showed moderate antibacterial activity against two gram-positive and three gram negative microorganisms tested with higher sensitivity for gram positive ones (Clear zone= 10mm for Bacillus cereus and 12mm for Staphylococcus aureus). Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were quantified by micro-dilution method. The MIC was 25 mg/ml for Bacillus cereus and 58 mg/ml for Escherichia coli and the MBC values were 400 and >400 mg/ml respectively for the mentioned species. Overall, results presented here suggest that the methanolic extract of Echinops robustus possesses antibacterial properties, and is, therefore, a potential source of active ingredients for food and pharmaceutical industry.

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Chemical composition, antimicrobial properties and toxicity evaluation of the essential oil of Cupressus lusitanica Mill. leaves from Cameroon

Cited by 33 publications

References 27 publications

Biosynthesis and application of silver nano-biotics for enhancing the antimicrobial activity of glass-ionomer cement against studied microorganisms and its effect on compressive strength

Biosynthesis and application of silver nano-biotics for enhancing the antimicrobial activity of glass-ionomer cement against studied microorganisms and its effect on compressive strength

Chemical composition, acute toxicity, antioxidant and anti-inflammatory activities of Moroccan Tetraclinis articulata L.

Chemical composition and antibacterial activity of methanolic extract from Echinops robustus on typical food-borne pathogens

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