Chemical composition and microbicidal activity of extracts from Brazilian and Bulgarian propolis

Salomão, Kelly; Dantas, A.P.; Borba, Cíntia M.; Campos, Leila Carvalho; Machado, D.G.; Neto, Francisco Radler de Aquino; Castro, Solange L. de

doi:10.1111/j.1472-765x.2003.01458.x

Cited by 107 publications

(83 citation statements)

References 23 publications

(25 reference statements)

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“…Comparison between the direct effect of Et-Blg against the three forms of the parasite showed that tissue-culture derived amastigotes were more susceptible than epimastigotes (Prytzyk et al 2003) and trypomastigotes (Salomão et al 2004). Such difference in susceptibility to Et-Blg among the three forms of T. cruzi has been already described in treatments with other drugs (Schlemper et al 1977, De Castro et al 1992, 1993.…”

Section: Discussionmentioning

confidence: 84%

“…It has been already shown propolis activity against pathogenic microorganisms that cause diseases in humans and animals (reviewed in , Prytzyk et al 2003, Salomão et al 2004). The present work encourages further investigations on the effect of propolis in experimentally T. cruzi-infected mice, aiming an alternative natural product for the treatment of Chagas disease.…”

Section: Discussionmentioning

confidence: 99%

“…In recent studies with a Bulgarian propolis sample, we have characterized by high temperature high resolution gas chromatography coupled to mass spectrometry (HT-HRGC-MS) the composition of ethanol and acetone extracts and assayed their activity against epimastigote and trypomastigote forms of T. cruzi, as well as on some species of fungi and bacteria of medical importance (Prytzyk et al 2003, Salomão et al 2004. In the present work we have analyzed the activity of the two extracts from this Bulgarian propolis sample against tissue culture-derived and intracellular amastigotes of T. cruzi, as well as ultrastructural alterations induced in epimastigote and trypomastigote forms of this parasite.…”

Section: Extracts From a Bulgarian Propolis With Known Chemical Compmentioning

confidence: 99%

“…Thus, our group has been engaged in experimental chemotherapy studies using propolis (Higashi & De Castro 1994, De Castro & Higashi 1995, Marcucci et al 2001, Prytzyk et al 2003, Paulino et al 2003, Salomão et al 2004). Propolis, a bee product collected from different plant exudates, is a complex mixture containing known bioactive constituents, such as flavonoids, phenolic acids and esters, terpenes, sesquiterpenes, aromatic aldehydes, and alcohols (Marcucci 1995 In temperate zones, bud exudates from poplar trees (Populus spp.)…”

Section: Extracts From a Bulgarian Propolis With Known Chemical Compmentioning

confidence: 99%

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The effect of Bulgarian propolis against Trypanosoma cruzi and during its interaction with host cells

Dantas

Salomão

Barbosa

et al. 2006

Mem. Inst. Oswaldo Cruz

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Propolis has shown activity against pathogenic microorganisms that cause diseases in humans and animals. The ethanol (Et-Blg) and acetone (Ket-Blg) extracts from a Bulgarian propolis, with known chemical compositions, presented similar activity against tissue culture-derived amastigotes. The treatment of Trypanosoma cruzi-infected skeletal muscle cells with Et-Blg led to a decrease of infection and of the intracellular proliferation of amastigotes, while damage to the host cell was observed only at concentration 12.5 times higher than those affecting the parasite. Ultrastructural analysis of the effect of both extracts in epimastigotes revealed that the main targets were the mitochondrion and reservosomes. Et-Blg also affected the mitochondrion-kinetoplast complex in trypomastigotes, offering a potential target for chemotherapeutic agents.Key words: propolis -natural products -Trypanosoma cruzi -Chagas disease Chagas disease, caused by the trypanosomatid protozoan Trypanosoma cruzi, is endemic in Latin America. Initially restricted to natural ecotopes, with transmission among small autochthonous mammals by hematophagous Triatominae bugs, the occupation by humans of such ecotopes led to the establishment of the vectors in human dwellings, changing the characteristic of the disease from zoonotic to antropozoonotic. In humans, the acute phase results in 2-8% mortality, specially among children, while in the chronic phase most patients remain asymptomatic and about 30 to 40% of the cases develop symptoms of cardiac or digestive lesions. The World Health Organization estimates that about 18 million people are infected with T. cruzi (WHO 2002). The available drugs for the clinical treatment, the nitroderivatives benznidazole and nifurtimox, are unsatisfactory. They present variable healing effects during the acute phase according to the geographical region and require long term treatment, besides presenting frequent toxic side effects and limited efficacy in the chronic stage (reviewed in Coura & De Castro 2001).In this context, there is an intense search for new synthetic compounds and natural products for treatment of Chagas disease. Thus, our group has been engaged in experimental chemotherapy studies using propolis (Higashi & De Castro 1994, De Castro & Higashi 1995, Marcucci et al. 2001, Prytzyk et al. 2003, Paulino et al. 2003, Salomão et al. 2004). Propolis, a bee product collected from different plant exudates, is a complex mixture containing known bioactive constituents, such as flavonoids, phenolic acids and esters, terpenes, sesquiterpenes, aromatic aldehydes, and alcohols (Marcucci 1995). In temperate zones, bud exudates from poplar trees (Populus spp.) are the main source of flavonoid-rich propolis, which are rich in flavonoids (reviewed in Bankova et al. 2000).During the last decades, an increasing number of studies on the chemical composition, biological activity, and therapeutic uses of propolis has been published (reviewed in De Castro 2001). In recent studies with a Bulgarian propolis sample, we ...

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Extracts From a Bulgarian Propolis With Known Chemical Compmentioning

confidence: 99%

Section: Extracts From a Bulgarian Propolis With Known Chemical Compmentioning

confidence: 99%

See 2 more Smart Citations

The effect of Bulgarian propolis against Trypanosoma cruzi and during its interaction with host cells

Dantas

Salomão

Barbosa

et al. 2006

Mem. Inst. Oswaldo Cruz

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“…The chemical composition of propolis is very complex and more than 300 constituents have been identified. It contains mainly resin (50-55%), wax (30%), essential oils (8-10%), organic material, minerals, pollen, and mechanical impurities [7][8][9]. In general, the composition of propolis is directly related to that of the bud exudates collected by the honeybees from various plants, and these bud exudates depend on many ecological factors [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Assessment of heavy metals in propolis and soil from the Pelagonia region, Republic of Macedonia

Popov

Hristova

Presilski

et al. 2017

Maced. J. Chem. Chem. Eng.

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In this study, the contents of five representative heavy metals (Cr, Pb, Zn, Cd and Cu) were determined in soil and propolis samples from four locations in southwestern Macedonia using atomic absorption spectrometry. The aim was to pinpoint the key factors that influence the content of heavy metals in propolis and to establish whether there is a connection between the contents of heavy metals in soil and in propolis from the same location. Generally, at all of the locations, the relative concentrations of heavy metals in soil were found to decrease in the following order: Zn>Cr>Cu>Pb>Cd. The highest mean values for concentrations of heavy metals in these soils were found to be: 72. 03, 38.28, 26.64, 17.15 and 0.60 mg kg -1 for Zn, Cr, Cu, Pb and Cd, respectively, and they are all below the target values from the new Dutch list. The general trend of the heavy metal contents in propolis from the same four locations, in decreasing order, is: Pb>Cr>Zn>Cu≈Cd. Generally, the propolis samples from the highland locations (Orle and Rapeš) had lower overall contents of heavy metals than the lowland locations (Novaci and Makovo). All of the analyzed propolis samples meet the requirements of the Macedonian legislation and the international organizations for the maximum allowed levels for heavy metals. Attempts were made to find a correlation between the heavy metal contents in soil and propolis. According to our aim, the investigation presented herein offers one step towards a complete picture of ecological safety of the specific areas in the Republic of Macedonia. To do so, it is necessary to perform additional studies and to find appropriate biomonitoring methods. Further studies are needed to complete the picture and to determine the major pathways of incorporation of heavy metals in beehive products.Key words: heavy metals; soil; propolis; atomic absorption spectrometry; honeybee; biomarker ПРОЦЕНА НА ТЕШКИ МЕТАЛИ ВО ПРОПОЛИС И ВО ПОЧВА ОД ПЕЛАГОНИСКИОТ РЕГИОН, РЕПУБЛИКА МАКЕДОНИЈАВо оваа студија, со користење на атомска апсорпциона спектрометрија, беше одредена содржината на пет тешки метали (Cr, Pb, Zn, Cd и Cu) во примероци почва и прополис, од четири локации во југозападниот дел на Македонија. Целта беше да се посочат клучните фактори кои влијаат на содржината на тешките метали во почвата и во прополис од иста локација. Општо земено, на сите локации релативните концентрации на тешки метали во почва беа со следниот опаѓачки редослед: Zn>Cr>Cu>Pb>Cd. Највисоките средни вредности од концентрациите на тешки метали во овие почви изнесуваат: 72,03; 38,28; 26,64; 17,15;0,60 mg kg -1 за Zn, Cr, Cu, Pb и Cd, соодветно, и сите тие се под целните вредности претставени во Холандската листа. Општиот тренд на содржината на тешки метали во прополисот од истите четири локации, е со следниот B. Bogdanova Popov, V. Karapetkovska Hristova, S. Presilski,M. A. Shariati, S. Najman Maced. J. Chem. Chem. Eng. 36(1), 23-33 (2017) 24 опаѓачки редослед: Pb>Cr>Zn>Cu≈Cd. Генерално, примероците од прополис од планинските локации (Орле и Ра...

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Chemical Properties and Antioxidant and Antimicrobial Activities of Slovenian Propolis

Mavri¹,

Abramovič²,

Polak³

et al. 2012

Chemistry & Biodiversity

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The chemical composition as well as the antioxidant and antimicrobial activities of two EtOH extracts of propolis (PEEs) from Slovenia were determined. EtOH was used as extracting solvent at 70 and 96%, providing the extracts PEE70 and PEE96, respectively. The extraction with 70% EtOH was more efficient than that with 96% EtOH, as the PEE70 was richer in total phenolic compounds than the PEE96. The Slovenian propolis was characterized by different phenolic acids and flavonoids. The PEE96 was slightly richer in three specific compounds, i.e., caffeic acid, ferulic acid, and luteolin, while all other substances detected showed higher contents in the PEE70. The PEE70 showed a stronger reducing power and ability to scavenge free radicals and metal ions than the PEE96. Both PEEs were in the main more effective against Gram-positive bacteria than against fungi and Gram-negative bacteria like Salmonella and Escherichia coli, with the exception of Campylobacter. The PEE96 decreased the intracellular oxidation in Saccharomyces cerevisiae in a dose-dependent manner. The antimicrobial activities and antioxidant properties were related to the total phenolic contents. The two PEEs have the potential for use as natural antimicrobial and antioxidant additives in foods.

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Chemical composition and microbicidal activity of extracts from Brazilian and Bulgarian propolis

Cited by 107 publications

References 23 publications

The effect of Bulgarian propolis against Trypanosoma cruzi and during its interaction with host cells

The effect of Bulgarian propolis against Trypanosoma cruzi and during its interaction with host cells

Assessment of heavy metals in propolis and soil from the Pelagonia region, Republic of Macedonia

Chemical Properties and Antioxidant and Antimicrobial Activities of Slovenian Propolis

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