Chemical composition and cytotoxic activity of the essential oil from the leaves of Casearia lasiophylla

Salvador, Marcos José; Carvalho, João Ernesto de; Wisniewski, Alberto; Kassuya, Cândida Aparecida Leite; Santos, Élide Pereira dos; Riva, Dilamara; Stefanello, Maria Élida Alves

doi:10.1590/s0102-695x2011005000073

Cited by 28 publications

(23 citation statements)

References 15 publications

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“…The evidence from this study, the high amount of terpenes, known for their anticancer activity such as β-caryophyllene (16.1%), aromadendrene oxide (14.7%), δ-selinene (13.6%), selin-11-en-4α-ol (12.5%), α-humulene (11.9%), β-caryophyllene oxide (4.1%), and α-cadinol (1.6%) ( Table 1), suggests that P. guajava essential oil is a significant potential source of pure compounds with promising anticancer activity (Salvador et al, 2011;Quassinti et al, 2013;Fidyt et al, 2016;Guerrini et al, 2016). Besides, despite the low concentration of hydrocarbon monoterpenes (1.8%) and oxygenated monoterpenes (1.2%) in the essential oil of P. guajava (Table 1), this class of compounds deserves special attention for its important antitumor activity (Sobral et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Antibacterial and antiproliferative activities of the fresh leaf essential oil of Psidium guajava L. (Myrtaceae)

et al. 2019

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This study evaluated the antibacterial and antiproliferative activities of the essential oil of Psidium guajava leaves (PG-EO), traditionally used in folk medicine. The essential oil was obtained from fresh leaves by hydrodistillation, using a modified Clevenger apparatus. The major PG-EO chemical constituents were identified by GC-MS and GC-FID as being β-caryophyllene (16.1%), α-humulene (11.9%), aromadendrene oxide (14.7%), δ-selinene (13.6%), and selin-11-en-4α-ol (12.5%). The antibacterial activity of the essential oil of P. guajava leaves was determined in terms of its minimum inhibitory concentrations (MIC) using the broth microdilution method in 96-well microplates. PG-EO had moderate activity against Streptococcus mutans (MIC = 200 µg/mL), S. mitis (MIC = 200 µg/mL), S. sanguinis (MIC = 400 µg/mL), S. sobrinus (MIC = 100 µg/mL), and S. salivarius (MIC = 200 µg/mL). The antiproliferative activity was evaluated against different tumor cell lines: breast adenocarcinoma (MCF-7), human cervical adenocarcinoma (HeLa), and human gliobastoma (M059J). A normal human cell line (GM07492A, lung fibroblasts) was included. The antiproliferative activity was evaluated using the XTT assay and the results were expressed as IC50. The essential oil showed significantly lower IC50 values against MCF-7 and M059J lines than that obtained for the normal line, showing selectivity. Our results suggest that the essential oil of Psidium guajava L. has promising biological activities and can be considered a new source of bioactive compounds.

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Section: Resultsmentioning

confidence: 99%

Antibacterial and antiproliferative activities of the fresh leaf essential oil of Psidium guajava L. (Myrtaceae)

et al. 2019

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“…Although there are no studies concernng the cytotoxic activity of sesquiterpenes isolated from oil of C. sylvestris , Salvador et al . [34] showed that essential oil from C. lasiophylla were cytotoxic against tumor cell lines U251, UACC-62, MCF-7, NC1-ADR/RES, NCI-H460, PC03, OVCAR-3, HT-29 K562. Silva et al .…”

Section: Resultsmentioning

confidence: 99%

Chemical Composition and Cytotoxicity Evaluation of Essential Oil from Leaves of Casearia Sylvestris, Its Main Compound α-Zingiberene and Derivatives

et al. 2013

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Casearia sylvestris (Salicaceae), popularly known as "guaçatonga", is a plant widely used in folk medicine to treat various diseases, including cancer. The present work deals with the chemical composition as well as the cytotoxic evaluation of its essential oil, its main constituent and derivatives. Thus, the crude essential oil from leaves of C. sylvestris was obtained using a Clevenger type apparatus and analyzed by GC/MS. This analysis afforded the identification of 23 substances, 13 of which corresponded to 98.73% of the total oil composition, with sesquiterpene α-zingiberene accounting for 50% of the oil. The essential oil was evaluated for cytotoxic activity against several tumor cell lines, giving IC 50 values ranging from 12 to 153 μg/mL. Pure α-zingiberene, isolated from essential oil, was also evaluated against the tumor cell lines showing activity for HeLa, U-87, Siha and HL60 cell lines, but with IC 50 values higher than those determined for the crude essential oil. Aiming to evaluate the effect of the double bonds of α-zingiberene on the cytotoxic activity, partially hydrogenated α-zingiberene (PHZ) and fully hydrogenated α-zingiberene (THZ) derivatives were obtained. For the partially hydrogenated derivative only cytotoxic activity to the B16F10-Nex2 cell line (IC 50 65μg/mL) was detected, while totally hydrogenated derivative showed cytotoxic activity for almost all cell lines, with B16F10-Nex2 and MCF-7 as exceptions and with IC 50 values ranging from 34 to OPEN ACCESSMolecules 2013, 18 9478 65 μg/mL. These results indicate that cytotoxic activity is related with the state of oxidation of compound.

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“…They identified about 25 constituents, the most predominant of which was the presence of germacrene D, bicyclogermacrene, δ-cadinene, spathulenol, caryophyllene oxide and β-caryophyllene at different levels 24 .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial potential of Casearia sylvestris against oral bacteria

Cavalheiro

Godoi

Carvalho

et al. 2016

Rev. odontol. UNESP

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ResumoObjetivo: O objetivo deste estudo foi obter extratos por diferentes métodos de extração de Casearia sylvestris, incluindo a obtenção de óleo essencial, a fim de comparar suas atividades antimicrobianas em relação aos enxaguatórios bucais convencionais com clorexidina contra bactérias orais. Material e método: Para esta avaliação, extratos das folhas foram obtidos por diferentes métodos de extração (infusão, decocção, maceração e percolação) utilizando diferentes sistemas de solventes: 100% de água, 100%, de etanol, metanol 100%, água: etanol 3: 7; água: etanol 7: 3; água: metanol 7: 3 e água: metanol 3: 7. O óleo essencial, que corresponde a uma fração volátil, foi obtido por hidrodestilação usando o aparelho de Clevenger modificado. O método de microdiluição em caldo foi usado para determinar os valores de concentração inibitória mínima (MIC) e a concentração bactericida mínima (MBC) para os seguintes microrganismos: Streptococcus mutans ATCC 25175, ATCC 49456 S. mitis, S. sanguinis ATCC 10556, S. salivarius ATCC 25975, Lactobacillus casei ATCC 11578 e Enterococcus faecalis ATCC4082. Gluconato de clorexidina foi usada como um controle positivo. Resultado: Todos os extratos avaliados no protocolo utilizado apresentaram valores de MIC superior a 400 ug/mL e alguns mostraram atividade bactericida. A atividade antimicrobiana do óleo essencial foi maior do que a atividade dos extratos, e a melhor concentração inibitória mínima e valores de concentração bactericida mínima foram obtidos contra L. casei (MIC de 0,023 ug/mL e MBC de 0,046 ug/mL) e S. mutans (MIC de 25 ug/mL e MBC de 50 ug /mL), respectivamente. Conclusão: O óleo essencial de Casearia sylvestris tem atividade antimicrobiana significativa contra microrganismos orais.Descritores: Pesquisa de laboratório; cáries dentárias; microrganismos; fitoterapia. AbstractAim: The aim of this study was to obtain Casearia sylvestris leave extracts by different extractive methods, including the obtention of essential oil, in order to compare their antimicrobial activities to conventional mouthwash chlorhexidine against oral bacteria. Material and method: For this evaluation, extracts from the leaves were obtained by different methods of extraction (infusion, decoction, maceration and percolation) using different solvent systems: water 100%, ethanol 100%, methanol 100%, water: ethanol 3:7; water: ethanol 7:3; water: methanol 7:3 and water: methanol 3:7. The essential oil, which corresponds to a volatile fraction, was obtained by hydrodistillation using Clevenger modified apparatus. The microdilution broth method was used to determine the values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for the following microorganisms: Streptococcus mutans ATCC 25175, S. mitis ATCC 49456, S. sanguinis ATCC 10556, S. salivarius ATCC 25975, Lactobacillus casei ATCC 11578 and Enterococcus faecalis ATCC4082. Chlorhexidine gluconate was used as a positive control. Result: All extracts evaluated in the used protocol displayed MIC values higher t...

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Chemical composition and cytotoxic activity of the essential oil from the leaves of Casearia lasiophylla

Cited by 28 publications

References 15 publications

Antibacterial and antiproliferative activities of the fresh leaf essential oil of Psidium guajava L. (Myrtaceae)

Antibacterial and antiproliferative activities of the fresh leaf essential oil of Psidium guajava L. (Myrtaceae)

Chemical Composition and Cytotoxicity Evaluation of Essential Oil from Leaves of Casearia Sylvestris, Its Main Compound α-Zingiberene and Derivatives

Antimicrobial potential of Casearia sylvestris against oral bacteria

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