Chemical Composition and, Cellular Evaluation of the Antioxidant Activity of Desmodium adscendens Leaves

Abstract

Desmodium adscendens plant is widely used as juice or tea in various parts of the world against a wide range of diseases. This study determines the quality and the quantity of polyphenols, flavonoids, anthocyanins, and tannins in D. adscendens leaves by UV-spectrophotometry and RP-HPLC methods. In addition, the antioxidant capacity of these phenolic compounds is evaluated by ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic)), DPPH (2,2-diphenyl-1 picrylhydrazyl), and Cellular tests. D. adscendens leaves are mainly composite of flavonoid compounds with 12.8 mg of catechin equivalent (CE)/g dw. The amounts of total polyphenol compounds are 11.1 mg of gallic acid equivalent (GAE)/g dw. The quantity of total anthocyanin and total tannin compounds is not considerable 0.0182 mg CgE/g dw and 0.39 mg CE/g dw, respectively. A direct correlation between phenolic compounds and antioxidant activity is observed (R 2 = 0.96). The RP-HPLC analyses reveal that the main phenolic compound identified in the methanol-water extract is quercetrin dihydrat (2.11 mg/mL). According to the results, it is observed that D. adscendens leaves possess a considerable scavenging antioxidant and antiradical capacity, therefore these antioxidant properties might increase the therapeutic value of this medicinal plant.

The genus Desmodium (Fabaceae)-traditional uses in Chinese medicine, phytochemistry and pharmacology

“…The methanol-water extract of Desmodium adscendens leaves was shown to possess a considerable scavenging antioxidant activity, the IC 50 values were 4.00 g/mL in both 2,2 -azino-bis(3-ethyl benzothiazoline-6-sulfonic and 2,2-diphenyl-1-picrylhydrazyl tests (Muanda et al, 2011).…”

“…Flavonoids and alkaloids are regarded as the major constituents and perhaps responsible for most of the activities shown by the plants of this genus (Ghosh and Anandakumar, 1983;Delle Monache et al, 1996;Khan et al, 2006;Gan et al, 2008;Ning et al, 2009). Furthermore, both in vivo and/or in vitro experiments have indicated that the Desmodium plant extracts possessed a wide range of pharmacological properties, including improving cardiovascular and cerebrovascular functions and regulating immune system as well as anti-inflammatory, cytotoxic, antiparasitic, antidiabetic, antinephrolithic, antibacterial, and nootropic activities (Ning et al, 2009;Kurian and Paddikkala, 2010;Zhu et al, 2010;Muanda et al, 2011).…”

Phytochemical screening, antioxidant, antiulcer and toxicity studies on <i>Desmodium adscendens </i>(Sw) DC Fabaceae leaf and stem

“…Also, compounds that have been isolated from the leaf include phenylethylamines, indole-3-alkyl amines, tetrahydroiso-quinolones, and triterpenoid saponins [4]. The major compounds reported in the volatile oil of the leaves were phytone (14.72 %), caryophyllene oxide (11.32 %), esdesma (7.41 %), geraniol (5.42 %), linalool (5.33 %), palmitic acid (5.06 %), α-caryophyllene (4.76 %), scytalone (3.83 %), β-ionone (3.47 %), 2,2-dimethyl-hexanale (3.37 %), pelargonaldehyde (3.26 %), hyperforine (3.27 %), 2-pentyl furan (2.71), oleic acid (2.68 %), and 4-azidoheptane (2.02%) [5].A literature research on the safety of D. adscendens extracts on renal, and hepatocytes cells in addition tothe protection against oxidative stress had been investigated. The report had shown that the concentrations of D. adscendens (1 and 10 mg/ml) were safe for HEPG2 and LLC-PK1 and also offers protection against oxidative stress in LLC-PK1 cells [6].…”

Desmodium adscendens . De l’usage traditionnel camerounais contre les hépatites à l’accompagnement des chimiothérapies

“…L'essai a été réalisé en utilisant une molécule de référence, l'acide chlorogénique et différentes doses d'extraits de Da.Ilaétédémontré que les extraits de Da possèdent une forte activté antiradicalaire/antioxydante, inhibant la fluorescence induite par H 2 O 2 de 81 % à la dose de 5m g/ml de lyophilisat, correspondant à 50 mg/kg de plante sèche [53]. Des travaux centréssur cette activité ont été repris [55] …”