Chemical Composition and Antioxidant, Anti-Inflammatory, and Antiproliferative Activities of Lebanese Ephedra Campylopoda Plant

Kallassy, Hany; Fayyad‐Kazan, Mohammad; Makki, Rawan; El-Makhour, Yolla; Rammal, Hassan; Léger, David Y.; Sol, Vincent; Fayyad‐Kazan, Hussein; Liagre, Bertrand; Badran, Bassam

doi:10.12659/msmbr.905056

Cited by 22 publications

(16 citation statements)

References 28 publications

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“…Terpenoid derivatives were also identified, mainly in the dichloromethane extract of E. chilensis (see Table 8). Among these, we found phytol which was also found in E. campylopoda [59]. This compound showed cytotoxic activity for a wide range of cancer cell lines, and particularly for the MCF-7 and PC-3 cancer cell lines [60].…”

Section: Discussionmentioning

confidence: 99%

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

Mellado

Soto

Madrid

et al. 2019

BMC Complement Altern Med

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Background: Ephedra chilensis K Presl, known locally as pingo-pingo, is a Chilean endemic plant used in traditional medicine as an anti-inflammatory and used in other treatments. However, unlike for the other Ephedra species, there have been no reports on the antioxidant and cytotoxic effects of this plant. The present study aims to explore the potential applications of E. chilensis extract as a cytotoxic agent against in vitro cancer cell lines and to explore the relationship between this extract and antioxidant activity. Methods: Total anthraquinone, flavonoid, and phenolic contents, as well as antioxidant activity (DPPH, FRAP, and TRAP assays) and cytotoxic effect on several cancer cell lines (MCF-7, PC-3, DU-145, and HT-29) were measured for the hexane, dichloromethane and ethanol extracts of E. chilensis. In addition, several correlations among the phytochemical content, antioxidant activity, and cytotoxic effect were evaluated. Finally, GC-MS analyses of the most active extracts were carried out to identify their major components and to relate these components to the cytotoxic effect. Results: Antioxidant activity was found in the EtOH extracts of Ephedra, and the results were correlated with the phenolic content. For the cytotoxic activity, the non-polar extracts of E. chilensis had the highest antiproliferative effect for the MCF-7 and PC-3 cancer lines; the extract was shown to be up to three times more selective than doxorubicin. However, the cytotoxic effect was not correlated with the antioxidant activity. Lastly, the GC-MS analysis showed a high concentration of saturated fatty acids (mainly n-hexadecanoic acid) and terpenoids (mainly 4-(hydroxy-ethyl)-γbutanolactone). Conclusion: The cytotoxic activity and selectivity of the non-polar extracts of E. chilensis for the MCF-7 and PC-3 cell lines could be related to the terpenic compounds and fatty acids of the extracts or to the synergistic effect of all of the compounds in the extracts. These non-polar extracts can be used for the development of new drugs against breast and prostate cancer.

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Section: Discussionmentioning

confidence: 99%

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

Mellado

Soto

Madrid

et al. 2019

BMC Complement Altern Med

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“…(Syn. E. campylopoda ), allowed the identification of vinyl guaiacol ( 39 ), syringol ( 40 ), di- tert -butylphenol ( 41 ), antiarol ( 42 ), and vitamin E ( 43 ) through liquid chromatography coupled to mass spectrometry (HPLC-MS/MS) analysis [ 91 ]. In recent years, the standardization of herbal products used in the Chinese traditional medicine has been implemented as a quality control tool to guarantee the effectiveness of these kind of products.…”

Section: Chemistrymentioning

confidence: 99%

A Review of the Ephedra genus: Distribution, Ecology, Ethnobotany, Phytochemistry and Pharmacological Properties

et al. 2020

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Ephedra is one of the largest genera of the Ephedraceae family, which is distributed in arid and semiarid regions of the world. In the traditional medicine from several countries some species from the genus are commonly used to treat asthma, cold, flu, chills, fever, headache, nasal congestion, and cough. The chemical constituents of Ephedra species have been of research interest for decades due to their contents of ephedrine-type alkaloids and its pharmacological properties. Other chemical constituents such as phenolic and amino acid derivatives also have resulted attractive and have provided evidence-based supporting of the ethnomedical uses of the Ephedra species. In recent years, research has been expanded to explore the endophytic fungal diversity associated to Ephedra species, as well as, the chemical constituents derived from these fungi and their pharmacological bioprospecting. Two additional aspects that illustrate the chemical diversity of Ephedra genus are the chemotaxonomy approaches and the use of ephedrine-type alkaloids as building blocks in organic synthesis. American Ephedra species, especially those that exist in Mexico, are considered to lack ephedrine type alkaloids. In this sense, the phytochemical study of Mexican Ephedra species is a promising area of research to corroborate their ephedrine-type alkaloids content and, in turn, discover new chemical compounds with potential biological activity. Therefore, the present review represents a key compilation of all the relevant information for the Ephedra genus, in particular the American species, the species distribution, their ecological interactions, its ethnobotany, its phytochemistry and their pharmacological activities and toxicities, in order to promote clear directions for future research.

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“…From a chemical point of view, previous studies conducted on Ephedra showed that it contained different types of polyphenols, flavonoids, and anthocyanins [41,42,[90][91][92][93][94][95][96][97]. For quantitative measurement, gallic acid (quercetin or catechin) and cyanidin-3-glucoside were used as standard compounds (references) to quantify total polyphenol, total flavonoid, and total anthocyanins content, respectively.…”

Section: Ephedra Extracts Phytochemical Contentmentioning

confidence: 99%

“…Hegazy et al [96] observed that the total anthocyanins content (TAC) of Saudi E. foeminea, collected from Shada Mountain, southwest Saudi Arabia, was 0.14 mg cy-3-glu/ 100 g. TAC of Lebanese E. campylopoda was detected in the MeOH extracts but not in EtOH and aqueous fractions [97].…”

Section: Ephedra Extracts Phytochemical Contentmentioning

confidence: 99%

A Review on Worldwide Ephedra History and Story: From Fossils to Natural Products Mass Spectroscopy Characterization and Biopharmacotherapy Potential

Elhadef

Smaoui

Fourati

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Growing worldwide, the genus Ephedra (family Ephedraceae) had a medicinal, ecological, and economic value. The extraordinary morphological diversity suggests that Ephedra was survivor of an ancient group, and its antiquity is also supported by fossil data. It has recently been suggested that Ephedra appeared 8–32 million years ago, and a few megafossils document its presence in the Early Cretaceous. Recently, the high analytical power provided by the new mass spectrometry (MS) instruments is making the characterization of Ephedra metabolites more feasible, such as ephedrine series. In this regard, the chemical compounds isolated from crude extracts, fractions, and few isolated compounds of Ephedra species were characterized by MS-based techniques (LC-MS, LC-ESI-MS, HPLC-PDA-ESI/MS, LC-DAD-ESI/MSn, LC/Orbitrap MS, etc.). Moreover, we carry out an exhaustive review of the scientific literature on biomedicine and pharmacotherapy (anticancer, antiproliferative, anti-inflammatory, antidiabetic, antihyperlipidemic, antiarthritic, and anti-influenza activities; proapoptotic and cytotoxic potential; and so on). Equally, antimicrobial and antioxidant activities were discussed. This review is focused on all these topics, along with current studies published in the last 5 years (2015–2019) providing in-depth information for readers.

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Chemical Composition and Antioxidant, Anti-Inflammatory, and Antiproliferative Activities of Lebanese Ephedra Campylopoda Plant

Cited by 22 publications

References 28 publications

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

A Review of the Ephedra genus: Distribution, Ecology, Ethnobotany, Phytochemistry and Pharmacological Properties

A Review on Worldwide Ephedra History and Story: From Fossils to Natural Products Mass Spectroscopy Characterization and Biopharmacotherapy Potential

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