Chemical Composition and Antimicrobial Activity of Comorian Ocimum canum Essential Oil Harvested in the Region of Maweni Dimani-Grande Comoros

Hassane, Said Omar Said; Farah, Abdellah; Satrani, Badr; Ghanmi, Mohamed; Chahmi, Naima; Soidrou, S.H.; Chaouch, Abdelaziz

doi:10.1007/978-90-481-8650-1_29

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…An improved distribution of lipids may make them more available to the nematodes and/or bacteria. Indeed, Hassane et al (2012) used agar at 0.2% to obtain a homogenous distribution of essential oils in culture media.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Nutrient Concentration, Addition of Thickeners, and Agitation Speed on Liquid Fermentation of Steinernema feltiae

Leite¹,

Shapiro‐Ilan²,

Hazır³

et al. 2016

Journal of Nematology

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Entomopathogenic nematode production in liquid fermentation still requires improvements to maximize efficiency, yield, and nematode quality. Therefore, this study was aimed at developing a more suitable liquid medium for mass production of Steinernema feltiae, by assessing the effects of nutrient concentration, thickeners (primarily agar), and agitation speed on infective juvenile (IJ) yield. Base medium (BM) contained yeast extract (2.3%), egg yolk (1.25%), NaCl (0.5%), and corn oil (4%). All media were inoculated with Xenorhabdus bovienii, and 2 d later, with 2-d-old S. feltiae juveniles. For the nutrient concentration experiment, we evaluated the base medium versus a modified base medium containing all the components, but with 33 concentrations of yeast extract (6.9%), egg yolk (3.75%), and corn oil (12%). The nematodes and bacteria were cultured in 150-ml Erlenmeyer flasks containing 50 ml of liquid medium at (258C) and 180 rpm on a rotary shaker incubator. To assess the effect of thickeners, IJs were inoculated in BM with agar (0.2%), carrageen (0.2%), and carboxymethyl cellulose (0.2% and 0.5%). The addition of 33 more nutrients relative to the BM resulted in a significantly lower yield of nematodes. For agar and agitation speed experiments, five levels of agar in the BM (0%, 0.2%, 0.4%, 0.6%, and 0.8% agar) and two agitation speeds (180 and 280 rpm) were evaluated for production. Increasing agitation speed from 180 to 280 rpm and higher levels of agar in the medium (. 0.2%) significantly increased the yield of bacteria. At the lower agitation speed, media amended with 0.4% and 0.6% agar produced higher nematode yields compared to media without agar. Media with 0.2% and 0.8% agar resulted in intermediate levels of nematode production. At the higher agitation speed, media supplemented with 0.8% agar resulted in the lowest yield of nematodes when compared to the other media tested. Results indicated that increasing nutrient concentration levels was detrimental to nematode production. Also, media containing agar (0.4% and 0.6%) increased nematode yields when cultures were grown at low agitation speed. When IJs were used as the inoculum, 0.2% agar also enhanced recovery and nematode yield at the higher agitation speed.

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Section: Discussionmentioning

confidence: 99%

The Effects of Nutrient Concentration, Addition of Thickeners, and Agitation Speed on Liquid Fermentation of Steinernema feltiae

Leite¹,

Shapiro‐Ilan²,

Hazır³

et al. 2016

Journal of Nematology

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“…(Lamiaceae), commonly known, as hoary basil, is widely distributed in tropical and subtropical parts of the world, but most prevalent throughout Indian subcontinent (Paton et al 1999). Traditionally, this species is widely used for medicinal propose as well as source of aromatic compounds and essential oils containing biologically active constituents against diabetes (Nyarko et al 2002), various bacteria, fungi (Hassane et al 2012;Saha et al 2013) and parasites (Jayaseelan and Rahuman 2012). However, a major difficulty in the use of Lamiaceous species like Ocimum spp.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

Saha

Roy

Sengupta

et al. 2014

Ind J Plant Physiol.

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An efficient and improved plant regeneration protocol has been developed from nodal explants of Ocimum canum Sims., a medicinally important herbaceous plant species belonging to the family Lamiaceae. Murashige and Skoog (MS) basal medium supplemented with 0.5 mg l -1 N 6 -benzyladenine (BA) showed maximum percentage of shoot multiplication (91.1 %) with 5.32 shoots per explant. Rooting of shoots occurred on 1/2 MS medium supplemented with 1.0 mg l -1 indole-3-butyric acid (IBA). Well-developed plantlets, transferred to plastic pots containing sand, soil and compost (2:2:1) showed 80.9 % survival rate under field condition. The genetic fidelity of in vitro raised field-grown plants to the donor plant was ascertained from histological, cytological and two PCR based molecular markers, i.e., random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR). Histological study showed direct organogenesis on epidermal and sub epidermal layer, where as cytological studies showed no visible anomalies within its chromosome compliments. Both RAPD and ISSR analysis revealed high degree of monomorphism, similar to those of the mother plants. This method of in vitro clonal propagation of O. canum Sims. may, therefore, could be an avenue of its sustainable commercial exploitation and conservation.

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“…As reported rosmanol and ferruginol are also responsible for the biological activity of sage along with the phenolic rosmarinic and salvianolic acids [43]. But, the antibacterial activity of S.officinalis extracts can also be due to the presence of interaction between different volatile constituents [44]. About Rosmarinus officinalis known for these medicinal virtues around the world [45], according to the results obtained in the present work, Genena and al in 2008 was mentioned that R. officinalis leaf extracts obtained by SFE extraction a good antibacterial activity against Gram+ bacteria (Staphylococcus aureus and Bacillus cereus) and Gram-bacteria (Escherichia coli) [46], two years later, other studies mentioned that the extract of R. officinalis has antibacterial activity against clinical Methicillin-resistant Staphylococcus aureus and against Listeria species such as Listeria grayi, Listeria innocua, Listeria ivanovii and Listeria monocytogene [47][48][49].…”

Section: B Discussionmentioning

confidence: 96%

The Antibacterial Activity Screening of the Extracts of Some Moroccan Medicinal Plants

Lahlou¹,

Amraoui²,

Bamhaoud³

2019

International Journal of New Technology and Research

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The antibacterial and antifungal activities of some Moroccan medicinal plants extracts were evaluated against six bacterial species and three yeasts. The agar diffusion method was used to evaluate the antimicrobial activity of the extracts of the following medicinal plants: Rosmarinus officinalis, Salvia officinalis, Psidium guajava, Crocus sativus, Dysphania ambrosioides, Trigonella foenum graecum and Cymbopogon citratus. The used standard antibiotic is the Ampicillin 30µg, and the standard antifungal is the Econazole30µg. The highest antibacterial activity was observed for the extracts of Rosmarinus offficinalis (16,6mm

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Chemical Composition and Antimicrobial Activity of Comorian Ocimum canum Essential Oil Harvested in the Region of Maweni Dimani-Grande Comoros

Cited by 6 publications

References 29 publications

The Effects of Nutrient Concentration, Addition of Thickeners, and Agitation Speed on Liquid Fermentation of Steinernema feltiae

The Effects of Nutrient Concentration, Addition of Thickeners, and Agitation Speed on Liquid Fermentation of Steinernema feltiae

Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

The Antibacterial Activity Screening of the Extracts of Some Moroccan Medicinal Plants

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