Chemical composition and antimicrobial activity of hexane leaf extract of Anisopus mannii (Asclepiadaceae)

Abstract

Objective:The aim was to determine the chemical constituents and antimicrobial activity of the hexane leaf extract of Anisopus mannii against a wide range of human pathogenic microorganisms.Methods:The chemical constituents of the hexane leaf extract was determined using gas chromatography-mass spectrometry (GC-MS) analysis; and the antimicrobial activity was evaluated on “standard strains”, clinical susceptible and resistant bacterial and fungal isolates using the disc diffusion and broth microdilution methods.Results:GC-MS analysis of the hexane leaf extract revealed 32 compounds, representing 73.8% of the identified components. The major compounds were hexadecanoic acid, ethyl ester (34%), oxirane, hexadecyl- (11%) and 9, 12, 15-octadecatrienoic acid, ethyl ester, (Z, Z, Z) (9.6%). Results from the antimicrobial activity demonstrated higher inhibition zones against Bacillus cereus (29 mm), followed by Streptococcus pyogenes (28 mm). Other notable inhibitions were observed with Enterococcus faecalis (27 mm), Proteus vulgaris (26 mm) and MRSA (25 mm). The MIC values ranged from 0.625 mg/mL to 1.25 mg/mL while the MBC/MFC values ranged from 2.5 mg/mL to 5.0 mg/mL.Conclusion:These results support the traditional use of the plant and demonstrate the huge potential of A. mannii as a source of antimicrobial compounds.

Green Synthesis of Hydrophobic Magnetite Nanoparticles Coated with Plant Extract and Their Application as Petroleum Oil Spill Collectors

“…The wide availability of this plant, fast extraction process, low cost, green character and diversity of compounds in these extracts ensure their compatibility as capping agents for the MNPs. The n -hexane and chloroform extracts usually contain different active compounds, such as fatty acids, sesquiterpenoids, diterpenoids, phenolic compounds, coumarins and terpenoids [ 38 , 39 , 40 ]. The presence of these compounds in the capping agent increased their ability to form colloidal particles in the crude oil by utilizing several interactions, such as the aromatic π–π* stacking force, hydrogen bonding, Van der Waals force and electrostatic attractions.…”

Ethyl Iso-allocholate from a Medicinal Rice Karungkavuni Inhibits Dihydropteroate Synthase in Escherichia coli: A Molecular Docking and Dynamics Study

“…The biological activities of these compounds are listed in Table 2 [23][24][25][26][27][28][29][30][31][32][33][34] . Reports suggest that compounds 9,12,15-octadecatrienoic acid-2,3-dihydroxy propyl ester [26] , sitosterol [28,29] , squalene [30,31] and ethyl iso-allocholate [33] have antimicrobial activity. We selected these four compounds for docking studies to reveal the binding orientation of ligands with the target.…”

Antibacterial and anti-biofilm activity of the lipid extract from Mantidis ootheca on Pseudomonas aeruginosa

“…The lipid extract from M. ootheca consists of more than 16 compounds, and the proportions of oleic acid, 9,12-octadecadienoic acid, and 9,12,15-octadecatrienoic acid collectively comprise up to 79.74% of the extract. Some extracts from other natural products containing oleic acid, 9,12octadecadienoic acid, or 9,12,15-octadecatrienoic acid were demonstrated to have antibacterial properties in previous studies (Clementi et al, 2013;Musa et al, 2015;Patra et al, 2015). However, whether the antibacterial function results from a single component or the synergistic action of several components has not yet been studied in detail.…”