Chemical composition and antibacterial properties of the essential oils and crude extracts of Merremia borneensis

Abstract

The hydro distilled essential leaves and stems oils of Merremia borneensis were analysed by GC-MS. Sixty-nine compounds representing 96.81% and 89.89% of the leaves and stems oils, respectively, were identified, of which chloromethyl propanoate (3.29% and 3.54%), methylcyclopropanemethanol (1.29% and 1.03%), oxirane (1.41% and 1.05%), 1-penten-3-ol (1.33% and 1.12%), 1-(2-propenyloxy)-heptane (3.44% and 2.98%), camphene (4.11% and 3.65%), l-octen-3-ol (1.56% and 1.08%), a-pinene (2.98% and 2.12%), b-pinene (2.19% and 1.93%), 2-methyl-2-nitropropane (11.91% and 10.51%), bis(1,1-dimethylethyl)-diazene (1.25% and 1.71%), p-cymene (2.23% and 2.11%), limonene (1.28% and 1.11%), neopentane (12.02% and 11.95%), cyclopropyl methyl carbinol (2.19% and 1.99%), cis-2-octenal (1.29% and 1.13%), 4-undecanone (4.11% and 3.99%), menthone (1.99% and 1.73%), isomenthone (1.01% and 0.93%), methylchavicol (1.57% and 2.22%), dodecane (1.01% and 0.72%), eugenol (3.12% and 3.09%), b-elemene (1.99% and 1.89%), methyleugenol (1.42% and 1.13%), b-carryophyllene (1.12% and 1.05%), a-humulene (6.54% and 6.32%), tridecane (1.16% and 1.08%) were the major compounds. Thus, different types of monoterpenes and sesquiterpenes were the predominant portions of the oils. Essential oils and methanol extract of M. borneensis and the derived fractions of hexane, chloroform, and ethyl acetate were tested for antibacterial activity, which was determined by disc diffusion and minimum inhibitory concentration (MIC) determination methods. The oils, methanol extract and derived fractions of methanol extract did not display any potential of antibacterial activity against the tested 10 phytopathogenic bacterial such as Enterobacter cloacae, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Salmonella typhimurium, Salmonella biafra, Klebsiella pneumoniae, Vibrio cholerae and Vibrio parahaemolyticus, in the range of 0% and minimum inhibitory concentration ranging from 25 to 100 lg/ml.

Postharvest Management and Value Addition of Ginger (Zingiber Officinale Roscoe): A Review

“…The aromatic constituents include zingiberene and bisabolene. Other gingerol-or shogaol-related compounds (1-10%), which have been reported in ginger rhizome, include 6-paradol, 1-dehydrogingerdione, 6-gingerdione and 10-gingerdione, 4-gingerdiol, 6-gingerdiol, 8-gingerdiol, and 10-gingerdiol, and diaryl-heptanoids (Michiein et al 2009, Elaissi et al 2011, Hossain et al 2011. The mode of action and active constituents present in ginger are listed in Table 1.…”

Evaluation of Antibacterial Activities of Citrus limon, Citrus reticulata, and Citrus grandis Against Pathogenic Bacteria

“…The extracts were transferred to glass vials and kept at 4°C before use. 11 Preparation of Different Concentrations of Methanolic Extracts Different plant extracts used in this study were dissolved in 80% methanol for evaluation of their antimicrobial susceptibility. The concentrations were as follows: C. reticulata 400 mg/mL, C. limon 150 mg/mL, and C. grandis 200 mg/mL.…”

“…All essential oils were dried over anhydrous sodium sulphate and stored in brown vials in the refrigerator at 4°C for the next step of test. 10,11 Preparation of Different Concentrations of Essential Oils The stock solution of essential oils was prepared by dissolving 10 mg of leaf essential oils of three Citrus species in 10 mL of 80% methanol. Eight dilutions of these essential oils were made for determination of minimum inhibitory concentration (MIC).…”

Evaluation of the Antimicrobial Activity of Tribulus terrestris, Allium sativum, Salvia officinalis, and Allium hirtifolium Boiss Against Enterococcus faecalis

“…An aliquot of the extract was heated at 121°C for 15 minutes using the aforementioned pressure autoclave for further evaluations as heated A. sativum extract. 21 Microorganism and its Maintenance Antibacterial assessment of aqueous extracts of the abovementioned plants were made by agar disc diffusion, using E. faecalis (PTCC 1394) obtained from the Persian Type Culture Collection. The bacterium was stored in trypticase soy broth containing 25% (v/v) glycerol (Merck, Germany) and refreshed on Mueller-Hinton agar (MHA) media (Hi media, India) before use.…”