Chemical characterization and immunostimulatory effects of a polysaccharide from Polygoni Multiflori Radix Praeparata in cyclophosphamide-induced anemic mice

Chen, Qin; Zhang, Song-zhao; Ying, Hua‐Zhong; Dai, Xiaojian; Li, Xiaoxian; Yu, Chunjing; Ye, Hai-chao

doi:10.1016/j.carbpol.2012.02.055

Cited by 56 publications

(29 citation statements)

References 33 publications

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“…The chemical bonds and chief functional groups of polysaccharides were showed in the FT‐IR spectrum of DIP‐1. As shown in Figure b, DIP‐1 displayed a broad band around 3,416.46 cm −1 , which exhibited O–H stretching vibration (Chen et al, ). The band at 2,927.76 cm −1 reflected the weak anti‐symmetrical C–H stretching vibration (Chen et al, ; You, Yin, Zhang, & Jiang, ).…”

Section: Resultsmentioning

confidence: 97%

“…As shown in Figure b, DIP‐1 displayed a broad band around 3,416.46 cm −1 , which exhibited O–H stretching vibration (Chen et al, ). The band at 2,927.76 cm −1 reflected the weak anti‐symmetrical C–H stretching vibration (Chen et al, ; You, Yin, Zhang, & Jiang, ). Band at 1,640.67 cm −1 was the bonded water's characteristic absorption and demonstrated the N–H of primary amine (Wang et al, ).…”

Section: Resultsmentioning

confidence: 97%

“…The extraction of crude polysaccharide was performed in accordance with the previous methods with some modifications (Chen et al, ; Seedevi et al, ). Briefly, the pretreated dry powders of D. indica (100 g) were soaked in distilled water for 1 hr.…”

Section: Methodsmentioning

confidence: 99%

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Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Xiang

Лі

et al. 2019

J Food Biochem

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A neutral polysaccharide (DIP‐1) from Duchesnea indica (Andr.) Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE‐52 cellulose anion‐exchange column and Sephadex G‐100 gel column). The physicochemical properties of DIP‐1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV–visible spectrophotometry, scanning electron microscope and Congo red test. The results indicated that DIP‐1 was consisted of mannose, glucosamine, glucose, galactose and arabinose in a ratio of 1.00:0.42:18.36:14.17:0.81, and its molecular weight was 218.3 kDa. Meanwhile, DIP‐1 presented a straight hexahedron structure, but no triple‐helical conformation. In antioxidant activity tests, DIP‐1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose‐dependent manner. Especially, DIP‐1 demonstrated high inhibitory activities against SKOV‐3 and Hep‐G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively. Practical applications D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on. At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity. In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time. Moreover, the neutral polysaccharide exhibits significant antioxidant and antitumor activities. Therefore, the present study laid a foundation for the high‐value application of D. indica polysaccharides in functional food and pharmaceutical industries.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

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Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Xiang

Лі

et al. 2019

J Food Biochem

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“…Heshouwu has been described as a dietary herbal medicine, rather than a therapeutic drug in the Wudang Taoist medicine system (6,7). Previous studies have demonstrated various biological effects for Heshouwu, including anti-aging, immunomodulatory, antihyperlipidemic, hepatoprotective, anticancer and anti-inflammatory effects, in addition to an ability to improve learning and memory (21)(22)(23)(24)(25)(26)(27)(28). At present, residents of the Wudang Mountain Area prefer to collect wild Heshouwu for their dietary and medicinal requirements, instead of purchasing it commercially (3,6,7).…”

Section: Discussionmentioning

confidence: 99%

Quality evaluation of Heshouwu, a Taoist medicine in Wudang, China

Cao

Wang

et al. 2016

Experimental and Therapeutic Medicine

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Thunb., which is known as Heshouwu in China, is a Taoist medicine sourced from the Wudang mountain area. At present, the quality of the Heshouwu sourced from this region is unknown. The present study aimed to evaluate the quality of wild Heshouwu collected from the Wudang mountain area, particularly the 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG) and combined anthraquinone (CAQ) content, compared with that of commercially available Heshouwu. Furthermore, the potential quantities of organic pesticide residues were determined. High performance liquid chromatography with a diode array detector was used to quantify TSG and CAQ content, whereas gas chromatography (GC), performed using a temperature gradient, was used to detect the presence of organochlorine, pyrethroid and organophosphorus pesticides. The average TSG content present in the wild Heshouwu from the Wudang mountain area and in the commercially available Heshouwu was 2.39 and 1.10%, respectively. In addition, the average content of CAQ in these was 1.41 and 3.46%, respectively. GC did not detect residues of organic pesticides in the wild Heshouwu, thus this plant met the criterion of the Chinese Pharmacopeia (2010 edition). The results of the present study indicated that wild Heshouwu from the Wudang mountain area may be suitable for use as a Chinese medicine across China.

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“…Afterwards, the acetylated derivative components of the totally hydrolyzed LEP-1 were detected by a GC-MS analyzer [16,17]. The chromatographic data was acquired using the NIST Mass Spectral Database (NIST11) with NIST MS search program v.2.0.…”

Section: Monosaccharide Composition Analysis Of Lep-1mentioning

confidence: 99%

Structural characterization and anti-hypoxia activity of an exopolysaccharide isolated from fermentation broth of Lachnum sp.

Zong

Surhio

et al. 2016

Process Biochemistry

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Chemical characterization and immunostimulatory effects of a polysaccharide from Polygoni Multiflori Radix Praeparata in cyclophosphamide-induced anemic mice

Cited by 56 publications

References 33 publications

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Quality evaluation of Heshouwu, a Taoist medicine in Wudang, China

Structural characterization and anti-hypoxia activity of an exopolysaccharide isolated from fermentation broth of Lachnum sp.

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