Chemical Characterization and Evaluation of the Hypoglycemic Effect of Fructooligosaccharides from Psacalium decompositum

Abstract

Psacalium decompositum (Gray) H.E. Rob. & Brett (Asteraceae), popularly known as "matarique," is a medicinal plant used in Mexico for the empirical treatment of diabetes mellitus. Previous studies with P. decompositum roots demonstrated that an aqueous fraction containing carbohydrates (WMP-fraction) exhibited a notable hypoglycemic effect in experimental animals. However, studies to identify these carbohydrates have not yet been performed. The goal of this investigation was to isolate and characterize the principal compounds in the WMP-fraction by spectroscopic and spectrometric analysis. Additionally, we sought to evaluate the hypoglycemic effect of these compounds in healthy and alloxan-induced diabetic mice. The results show that the chemical structures of the major compounds in the WMP-fraction correspond with fructan-type oligosaccharides and have a hypoglycemic effect in healthy and diabetic mice.

Effect of Fructooligosaccharides Fraction from Psacalium decompositum on Inflammation and Dyslipidemia in Rats with Fructose-Induced Obesity

“…After this time, the normal group was kept under the same conditions, while the second group was divided in three groups: the Fru group treated with isotonic saline solution (4 mL/kg/day), the Fru + Beza group treated with bezafibrate (30 mg/kg/day, positive control), and the Fru + FOS group treated with FOS fraction (150 mg/kg/day); all treatments were administered intragastrically and doses were determined based on previous studies of FOS fraction [4]. Groups Fru, Fru + Beza and Fru + FOS were maintained under initial diet conditions and treatments were administrated for 12 weeks.…”

“…The major material content isolated was located in fractions 89–91, according to their HPTLC profiles, resulting in a white amorphous solid component. The identity of this component present in the 89–91 fractions was established by nuclear magnetic resonance ( 1 H and 13 C) [ 4 ].…”

“…10 mg of the FOS fraction were dissolved in D 2 O and analyzed on a Varian Gemini spectrometer operating at 500 MHz ( 1 H) and 125 MHz ( 13 C). Chemical shifts were reported in δ units (ppm) and compared with those previously described [ 4 ].…”

“…Jimenez-Estrada et al . [ 4 ] have reported that the aqueous fraction contains a carbohydrate-type fructan (inulin), which showed hypoglycemic effect in healthy and alloxan-induced diabetic mice. While the hypoglycemic properties of roots of P .…”

Chemoinformatic Analysis of Selected Cacalolides from Psacalium decompositum (A. Gray) H. Rob. & Brettell and Psacalium peltatum (Kunth) Cass. and Their Effects on FcεRI-Dependent Degranulation in Mast Cells

“…Interestingly, the decoction of the roots from P. decompositum has shown clear hypoglycemic effects in healthy mice and in transtiently hyperglycemic rabbits [7]. Besides, polysaccharide fractions obtained from the freeze-dried water extract from P. decompositum significantly reduced fasting blood glucose in mild alloxan-diabetic mice suggesting that the aqueous fraction, containing fructan-type oligosaccharides, is responsible for the hypoglycemic effects observed [8,10]. Recently, treatment with a fructooligosaccharides fraction from P. decompositum was shown to significantly reduce cholesterol, triglycerides, IL-6, IFN-γ, MCP-1, IL-1β and VEGF levels, as well as a decrease in body weight in an animal model of obesity.…”