Chemical characterization and antioxidant potential of ecuadorian propolis

Cuesta‐Rubio, Osmany; Hernández, Ingrid Márquez; Fernández, Mercedes Campo; Rodríguez-Delgado, Irán; Porto, Rodny Montes de Oca; Piccinelli, Anna Lisa; Celano, Rita; Rastrelli, Luca

doi:10.1016/j.phytochem.2022.113415

Cited by 6 publications

(4 citation statements)

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“…Furthermore, in a study conducted in China, the TPC of Chinese propolis, Baccharis propolis and Eucalyptus propolis was determined as 192.80, 135.07, and 217.70 mg GAE/g propolis, respectively [32] . In addition, two different studies showed that the total amount of phenolic substances varies in different numbers of propolis obtained from various regions of Poland (150.05–197.14 mg GAE/g propolis), and Ecuador (20.75–172.69 mg GAE/g dry propolis extract) [33,34] . In a study conducted in Türkiye, the inhibition properties of the ethanolic extracts of Ankara, Kars, Erzurum, Zonguldak, Düzce, and Giresun propolis were investigated.…”

Section: Resultsmentioning

confidence: 99%

“…[32] In addition, two different studies showed that the total amount of phenolic substances varies in different numbers of propolis obtained from various regions of Poland (150.05-197.14 mg GAE/g propolis), and Ecuador (20.75-172.69 mg GAE/g dry propolis extract). [33,34] In a study conducted in Türkiye, the inhibition properties of the ethanolic extracts of Ankara, Kars, Erzurum, Zonguldak, Düzce, and Giresun propolis were investigated. In the study, the TPC of propolis extracts was found to be between 114.207 and 261.055 mg GAE/g propolis.…”

Section: Antiviral Activity Assay Resultsmentioning

confidence: 99%

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Chemical Analysis and in Vitro Antiviral Effects of Northeast Türkiye Propolis Samples against HSV‐1

Cora

Buruk

Ünsal

et al. 2023

Chemistry & Biodiversity

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Propolis is one of the mixtures with the widest biological activity among natural products used in complementary medicine. HSV‐1 is a highly contagious and endemic virus. Available drugs are insufficient for recurrent HSV‐1 infections. Therefore, new approaches to treat HSV‐1 infections are still being developed. In this study, it was aimed to investigate the inhibition effect of ethanolic Anatolian propolis extracts obtained from the Eastern Black Sea Region (Pazar, Ardahan, and Uzungöl) on HSV‐1. In addition to the total phenolic (TPC) and the total flavonoid content (TFC), the phenolic profiles of the extracts were analyzed by HPLC‐UV. The antiviral activity of the extracts were tested by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), quantitative Real Time Polymerase Chain Reaction (qRT‐PCR), and plaque reduction tests, and the results were evaluated statistically. It was determined that the total amount of phenolic substances varied between 44.12 and 166.91 mg GAE/g, and the total flavonoid content of the samples varied between 12.50 and 41.58 (mg QUE/g). It was shown that all propolis samples used in the current study were effective against HSV‐1, but the higher phenolic compounds contained in the samples showed the higher activity. The results show that ethanolic propolis extracts are promising candidates for HSV‐1 treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Antiviral Activity Assay Resultsmentioning

confidence: 99%

Chemical Analysis and in Vitro Antiviral Effects of Northeast Türkiye Propolis Samples against HSV‐1

Cora

Buruk

Ünsal

et al. 2023

Chemistry & Biodiversity

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“…Clusia genus species thus far, with C. minor now added as another potential source of that phytochemical. A comprehensive review focusing on nemorosone effects recently has been published 44 considering its potential pharmacological effects including cytotoxicity against malignant cells and antiparasitic and antimicrobial properties.…”

Section: Resultsmentioning

confidence: 99%

“…Although other Clusiaceae genera are also reported for yielding bicyclic polyprenylated acylphloroglucinols (BPAPs), nemorosone has only been described in the name‐giving Clusia genus species thus far, with C. minor now added as another potential source of that phytochemical. A comprehensive review focusing on nemorosone effects recently has been published 44 considering its potential pharmacological effects including cytotoxicity against malignant cells and antiparasitic and antimicrobial properties.…”

Section: Resultsmentioning

confidence: 99%

A multiplex approach of MS, 1D‐, and 2D‐NMR metabolomics in plant ontogeny: A case study on Clusia minor L. organs (leaf, flower, fruit, and seed)

Noleto‐Dias,

Farag,

Porzel

et al. 2023

Phytochemical Analysis

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IntroductionThe genus Clusia L. is mostly recognised for the production of prenylated benzophenones and tocotrienol derivatives.ObjectivesThe objective of this study was to map metabolome variation within Clusia minor organs at different developmental stages.Material and MethodsIn total 15 organs/stages (leaf, flower, fruit, and seed) were analysed by UPLC‐MS and 1H‐ and heteronuclear multiple‐bond correlation (HMBC)‐NMR‐based metabolomics.ResultsThis work led to the assignment of 46 metabolites, belonging to organic acids(1), sugars(2) phenolic acids(1), flavonoids(3) prenylated xanthones(1) benzophenones(4) and tocotrienols(2). Multivariate data analyses explained the variability and classification of samples, highlighting chemical markers that discriminate each organ/stage. Leaves were found to be rich in 5‐hydroxy‐8‐methyltocotrienol (8.5 μg/mg f.w.), while flowers were abundant in the polyprenylated benzophenone nemorosone with maximum level detected in the fully mature flower bud (43 μg/mg f.w.). Nemorosone and 5‐hydroxy tocotrienoloic acid were isolated from FL6 for full structural characterisation. This is the first report of the NMR assignments of 5‐hydroxy tocotrienoloic acid, and its maximum level was detected in the mature fruit at 50 μg/mg f.w. Seeds as typical storage organ were rich in sugars and omega‐6 fatty acids.ConclusionTo the best of our knowledge, this is the first report on a comparative 1D‐/2D‐NMR approach to assess compositional differences in ontogeny studies compared with LC‐MS exemplified by Clusia organs. Results derived from this study provide better understanding of the stages at which maximal production of natural compounds occur and elucidate in which developmental stages the enzymes responsible for the production of such metabolites are preferentially expressed.

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