Chemical characterization and anti-inflammatory effect of rauvolfian, a pectic polysaccharide of Rauvolfia callus

Popov, Sergey; Винтер, В. Г.; Patova, Olga A.; Markov, Pavel A.; Nikitina, I. R.; Оводова, Р. Г.; Popova, G. Yu.; Shashkov, A. S.; Ovodov, Yu. S.

doi:10.1134/s0006297907070139

Cited by 11 publications

(7 citation statements)

References 27 publications

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“…Baill. (Luo Fu Mu in Chinese), which belongs to the family Apocynaceae, has been commonly used as a traditional Chinese medicine (TCM) for centuries to treat hypertension, snake bites, inflammation, and pruritus, among other diseases (31)(32)(33). R. verticillata is primarily distributed in the Yunnan and Guangxi provinces of China, India, and other tropical regions worldwide.…”

Section: Introductionmentioning

confidence: 99%

Reserpine Inhibit the JB6 P+ Cell Transformation Through Epigenetic Reactivation of Nrf2-Mediated Anti-oxidative Stress Pathway

Bao

Zhang

et al. 2016

AAPS J

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Abstract. Nuclear factor erythroid-2 related factor 2 (Nrf2) is a crucial transcription factor that regulates the expression of defensive antioxidants and detoxification enzymes in cells. In a previous study, we showed that expression of the Nrf2 gene is regulated by an epigenetic modification. Rauvolfia verticillata, a traditional Chinese herbal medicine widely used in China, possesses anticancer and antioxidant effects. In this study, we investigated how Nrf2 is epigenetically regulated by reserpine, the main active component in R. verticillata, in mouse skin epidermal JB6 P+ cells. Reserpine induced ARE (antioxidant response element)-luciferase activity in HepG2-C8 cells. Accordingly, in JB6 P+ cells, it upregulated the mRNA and protein levels of Nrf2 and its downstream target genes heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO1), while it only increased the protein level of UDP-glucuronosyltransferase 1A1 (UGT1A1). Furthermore, reserpine decreased the TPA (12-O-tetradecanoylphorbol-13-acetate)-induced colony formation of JB6 cells in a dosedependent manner. DNA sequencing and methylated DNA immunoprecipitation further demonstrated the demethylation effect of reserpine on the first 15 CpGs of the Nrf2 promoter in JB6 P+ cells. Reserpine also reduced the mRNA and protein expression of DNMT1 (DNA methyltransferase 1), DNMT3a (DNA methyltransferases 3a), and DNMT3b (DNA methyltransferases 3b). Moreover, reserpine induced Nrf2 expression via an epigenetic pathway in skin epidermal JB6 P+ cells, enhancing the protective antioxidant activity and decreasing TPA-induced cell transformation. These results suggest that reserpine exhibits a cancer preventive effect by reactivating Nrf2 and inducing the expression of target genes involved in cellular protection, potentially providing new insight into the chemoprevention of skin cancer using reserpine.

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Section: Introductionmentioning

confidence: 99%

Reserpine Inhibit the JB6 P+ Cell Transformation Through Epigenetic Reactivation of Nrf2-Mediated Anti-oxidative Stress Pathway

Bao

Zhang

et al. 2016

AAPS J

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“…var. has 135 species in the world, and 9 species in China, and is widely distributed in tropical and subtropical areas. PP is the acidic polysaccharide of molecular weight 50–300 kD.…”

Section: Discussionmentioning

confidence: 99%

“…Because the biological activity of PP is closely related to its structure, PP from different plants with different structures vary in their biological activities. In 2007, Popov et al extracted PP (rauvolfian RS) from R. verticillata (Lour) Baill. var.…”

Section: Discussionmentioning

confidence: 99%

“…hainanensis Tsiang was obtained from the Jianfengling National Forest Park of Ledong Li autonomous county, Hainan province, China. PP was extracted as per the previous report with minor modification . Briefly, 5 g of rootstock dried at 40°C was treated with diluted HCl (up to pH 4.4) at 50°C for 3 hours, and then extracted with 0.7% aqueous ammonium oxalate.…”

Section: Methodsmentioning

confidence: 99%

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Pectic polysaccharides extracted from Rauvolfia verticillata (Lour.) Baill. var. hainanensis Tsiang ameliorate ulcerative colitis via regulating the MAPKs and NF‐κB pathways in dendritic cells

Miao

Sun²,

et al. 2018

Clin Exp Pharma Physio

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This study was to investigate the effects and mechanisms of pectic polysaccharides (PP) extracted from Rauvolfia verticillata (Lour.) Baill. var. hainanensis Tsiang on dextran sulphate sodium (DSS)-induced ulcerative colitis (UC). Eighty female BALB/c mice were randomly divided into four groups: Control, DSS, DSS + salicylazosulfapyridine (SASP), and DSS+ PP. The disease activity index (DAI), overall physical activity, and blood stool were monitored daily to evaluate severity of UC. Histological scores of the colon were observed. The expression of nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPKs) pathways in colon tissues and bone marrow-derived dendritic cells (DCs) was assessed by western blot, immunohistochemistry, electrophoretic mobility shift assay (EMSA) and real time polymerase chain reaction (RT-PCR). Cytokines were measured by enzyme-linked immunosorbent assay (ELISA). The overall physical activity, DAI and histological scores decreased in DSS+SASP and DSS+PP groups, compared with the DSS-alone group. Also, tumour necrosis factor α (TNF-α) and interleukin 6 (IL-6) reduced significantly while the expression of IκBα was up-regulated, extracellular signal-regulated kinase (ERK), Jun N-terminal kinase (JNK) and p38 were activated, in DSS+SASP and DSS+PP groups. PP inhibited activation of MAPKs and NF-κB pathways in the bone-marrow-derived DCs. In conclusion, PP significantly ameliorated murine DSS-induced UC model, via regulation of MAPKs and NF-κB pathways in DCs.

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“…The active components of this medicinal plant are pectin polysaccharides (PPs) and alkaloids (7). Popov et al documented that PPs from Rauvolfia inhibited the progression of colon ulcers and reduced rectal myeloperoxidase levels in mice (8). However, their mechanisms of action have not been established.…”

Section: Original Articlementioning

confidence: 99%

Pectic polysaccharides ameliorate the pathology of ulcerative colitis in mice by reducing pyroptosis

Cui¹,

Yuan²,

Chen³

et al. 2022

Ann Transl Med

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Background: Ulcerative colitis (UC) is an inflammatory bowel disease which seriously affects the quality of life of patients. There has been an increasing amount of research related to the therapeutic effects and mechanisms of natural plant substances in the treatment of recurrent UC. Rauwolfia verticillata var.Hainanensis is a medicinal plant that is native to Hainan Island, China. Some studies have documented that pectic polysaccharides (PPs) from Rauvolfia inhibited the progression of colon ulcers. However, their mechanisms of action have not been established. Studies have revealed that suppressing pyroptosis can attenuate the damage of experimental colitis. However, it is unclear whether PPs from Rauvolfia verticillata inhibit inflammation through pyroptosis. This study investigated the effects and potential mechanisms of PPs extracted from Rauvolfia verticillata on experimental UC in mice.Methods: Male C57 mice (6-8 weeks old) were allocated into the control group, the dextran sulfate sodium (DSS)-induced UC model group (DSS group), or the DSS with pectic polysaccharides treatment group (DSS + PP group). The body weights, rectal bleeding, and stool consistencies in the mice were observed, and the disease activity index (DAI) score was calculated. Colon tissues were collected for pathological analysis by histological hematoxylin and eosin (H&E) staining. The levels of caspase-1 and interleukin (IL)-1β were detected by immunohistochemistry. Pyroptosis was assessed by transmission electron microscopy.Results: UC in mice induced by DSS resulted in decreased general physical activity and body weight, increased DAI score, significant histological changes, inhibited caspase-1 and IL-1β expression, and promoted pyroptosis. These DSS-induced changes could be partially ameliorated by administration of PP.Conclusions: PPs exerted an ameliorative effect on DSS-induced UC in mice by reducing pyroptosis.

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Chemical characterization and anti-inflammatory effect of rauvolfian, a pectic polysaccharide of Rauvolfia callus

Cited by 11 publications

References 27 publications

Reserpine Inhibit the JB6 P+ Cell Transformation Through Epigenetic Reactivation of Nrf2-Mediated Anti-oxidative Stress Pathway

Reserpine Inhibit the JB6 P+ Cell Transformation Through Epigenetic Reactivation of Nrf2-Mediated Anti-oxidative Stress Pathway

Pectic polysaccharides extracted from Rauvolfia verticillata (Lour.) Baill. var. hainanensis Tsiang ameliorate ulcerative colitis via regulating the MAPKs and NF‐κB pathways in dendritic cells

Pectic polysaccharides ameliorate the pathology of ulcerative colitis in mice by reducing pyroptosis

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