Pectic polysaccharides extracted from <i>Rauvolfia verticillata</i> (Lour.) Baill. var. hainanensis Tsiang ameliorate ulcerative colitis via regulating the <scp>MAPK</scp>s and <scp>NF</scp>‐κB pathways in dendritic cells

Miao, Xiaoqing; Sun, Xiulian; Li, Qiong-Si; Cui, Lu-Jia; Wang, Xuan-Yu; Zhuang, Gui-Feng; Deng, Tao-Zhi

doi:10.1111/1440-1681.13026

Cited by 16 publications

(5 citation statements)

References 24 publications

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“…Its roots and leaves are used for medicinal purposes including treating hypertension, hyperthermia, insomnia, and so on (Hong et al, 2012). Miao et al (2019) obtained a pectic polysaccharide PP from the rootstocks of R. verticillata and found that PP treatment attenuated the overall physical activity, DAI, and morphological damage in DSS-treated colitis mice. Meanwhile, PP dramatically reduced TNF-α, IL-6, and MPO activity.…”

Section: Rauvolfia Verticillatamentioning

confidence: 99%

Natural-Derived Polysaccharides From Plants, Mushrooms, and Seaweeds for the Treatment of Inflammatory Bowel Disease

Zhao

et al. 2021

Front. Pharmacol.

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Inflammatory bowel disease (IBD) is a chronic inflammatory disease impairing the gastrointestinal tract, and its incidence and prevalence have been increasing over time worldwide. IBD greatly reduces peoples' quality of life and results in several life-threatening complications, including polyp, toxic colonic dilatation, intestinal perforation, gastrointestinal bleeding, and cancerization. The current therapies for IBD mainly include drugs for noncritical patients and operation for critical patients. However, continuous use of these drugs causes serious side effects and increased drug resistance, and the demand of effective and affordable drugs with minimal side effects for IBD sufferers is urgent. Natural-derived polysaccharides are becoming a research hotspot for their therapeutic effects on IBD. This study focuses on the research progress of various natural polysaccharides from plants, seaweeds, and mushrooms for the treatment of IBD during recent 20 years. Regulation of oxidative stress, inflammatory status, gut microbiota, and immune system and protection of the intestinal epithelial barrier function are the underlying mechanisms for the natural-derived polysaccharides to treat IBD. The excellent efficacy and safety of polysaccharides make them promising candidates for IBD therapy.

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Section: Rauvolfia Verticillatamentioning

confidence: 99%

Natural-Derived Polysaccharides From Plants, Mushrooms, and Seaweeds for the Treatment of Inflammatory Bowel Disease

Zhao

et al. 2021

Front. Pharmacol.

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“…It has been well established that TLR4-mediated activation of NF-κB, a vital transcript factor involved in inflammation, is crucial in regulating intestinal inflammation. [27][28][29] Activation of the NF-κB signaling pathway in macrophages is associated with increased production of proinflammatory cytokines. 30 We have previously found that TLR4 is one of the important regulators of the NF-κB pathway in macrophages.…”

Section: Conflict Of Interestsmentioning

confidence: 99%

PTPRO exaggerates inflammation in ulcerative colitis through TLR4/NF‐κB pathway

Zhao

Yan

Zhu

et al. 2019

J of Cellular Biochemistry

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Previous studies have implicated protein tyrosine phosphatase receptor type O (PTPRO) as a key regulator in inflammation‐associated diseases; however, its role in ulcerative colitis (UC) remains largely unknown. Thus, we aim to elucidate the potential role and underlying mechanism of PTPRO in UC. In this study, increased expression of PTPRO, toll‐like receptor (TLR4) and inflammatory cytokines were observed in mucosal tissues (MTs) from inflamed areas and lamina propria mononuclear cells (LPMCs) of patients with UC compared with those from healthy controls. Then, it was manifested that PTPRO promoted the expression of TLR4 and proinflammatory cytokines in lipopolysaccharide‐induced (LPS‐induced) inflammatory macrophage model. Besides, PTPRO inhibited the proliferation of intestinal epithelial cells (IECs) but enhanced the apoptosis of IECs in macrophages. Moreover, levels of phosphorylated nuclear factor κB (NF‐κB)/p65 and inhibitor of NF‐κB α (IκBα) were more significantly increased in PTPRO overexpressed macrophages. In addition, the area under receiver operating characteristic curve was 0.807 (95%CI = 0.686‐0.958, P < .001) suggesting PTPRO as an ideal diagnostic marker for UC. Taken these, the present study shows strong evidence that PTPRO exaggerates inflammation in UC via TLR4/NF‐κB signaling pathway.

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“…Since they are food polysaccharides, they are a less toxic and effective therapeutic option (20). Studies have shown that PPs from Rauvolfia verticillata have therapeutic effects against UC (7)(8)(9)(10). Intestinal mucosal immunity plays an important role in the pathogenesis of intestinal inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…However, their mechanisms of action have not been established. Previous studies have shown that Rauvolu hainanensis extracts, especially PPs, inhibited the progression of dextran sulfate sodium (DSS)-induced colitis in mice by regulating the nuclear factor (NF)-κB pathway and interleukin (IL)-17 (9,10). Other reports have demonstrated that its mechanisms may be associated with the NF-κB and mitogen-activated protein kinase (MAPK) signaling pathways in which dendrites derived from mouse bone marrow shape cells play an essential role (10).…”

Section: Original Articlementioning

confidence: 99%

“…The mice were housed under standard conditions and maintained in a 12-hour light/dark cycle at 23±1 ℃ with free access to food and water. A total of 18 mice were randomly distributed into 3 groups of 6 mice each: the control group (normal control), in which the mice were administered ultrapure water; the DSS group, in which mice were administered 5% DSS aqueous solution for 5 days; and the DSS + PP group, in which mice were administered DSS aqueous solution for 5 days followed by intragastric administration of 100 μL PP solution (100 mg/kg/d) for 7 consecutive days (9,10). The 5% DSS aqueous solution was prepared using ultrapure water.…”

Section: Animal Modelsmentioning

confidence: 99%

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Pectic polysaccharides ameliorate the pathology of ulcerative colitis in mice by reducing pyroptosis

Cui¹,

Yuan²,

Chen³

et al. 2022

Ann Transl Med

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Background: Ulcerative colitis (UC) is an inflammatory bowel disease which seriously affects the quality of life of patients. There has been an increasing amount of research related to the therapeutic effects and mechanisms of natural plant substances in the treatment of recurrent UC. Rauwolfia verticillata var.Hainanensis is a medicinal plant that is native to Hainan Island, China. Some studies have documented that pectic polysaccharides (PPs) from Rauvolfia inhibited the progression of colon ulcers. However, their mechanisms of action have not been established. Studies have revealed that suppressing pyroptosis can attenuate the damage of experimental colitis. However, it is unclear whether PPs from Rauvolfia verticillata inhibit inflammation through pyroptosis. This study investigated the effects and potential mechanisms of PPs extracted from Rauvolfia verticillata on experimental UC in mice.Methods: Male C57 mice (6-8 weeks old) were allocated into the control group, the dextran sulfate sodium (DSS)-induced UC model group (DSS group), or the DSS with pectic polysaccharides treatment group (DSS + PP group). The body weights, rectal bleeding, and stool consistencies in the mice were observed, and the disease activity index (DAI) score was calculated. Colon tissues were collected for pathological analysis by histological hematoxylin and eosin (H&E) staining. The levels of caspase-1 and interleukin (IL)-1β were detected by immunohistochemistry. Pyroptosis was assessed by transmission electron microscopy.Results: UC in mice induced by DSS resulted in decreased general physical activity and body weight, increased DAI score, significant histological changes, inhibited caspase-1 and IL-1β expression, and promoted pyroptosis. These DSS-induced changes could be partially ameliorated by administration of PP.Conclusions: PPs exerted an ameliorative effect on DSS-induced UC in mice by reducing pyroptosis.

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Pectic polysaccharides extracted from Rauvolfia verticillata (Lour.) Baill. var. hainanensis Tsiang ameliorate ulcerative colitis via regulating the MAPKs and NF‐κB pathways in dendritic cells

Cited by 16 publications

References 24 publications

Natural-Derived Polysaccharides From Plants, Mushrooms, and Seaweeds for the Treatment of Inflammatory Bowel Disease

Natural-Derived Polysaccharides From Plants, Mushrooms, and Seaweeds for the Treatment of Inflammatory Bowel Disease

PTPRO exaggerates inflammation in ulcerative colitis through TLR4/NF‐κB pathway

Pectic polysaccharides ameliorate the pathology of ulcerative colitis in mice by reducing pyroptosis

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