Chemical Characterization and Anti-inflammatory Assessment of the Hydroethanolic Extract of Fridericia chica

Abstract

Fridericia chica (Bonpl.) L.G. Lohmann, Bignoniaceae, is an Amazonian species known as "pariri" or "crajiru" that is included in the Brazilian National List of Medicinal Plants of Interest to the Unified Health System (Renisus). This herbal remedy is traditionally used as an infusion to treat diarrhea, anemia, inflammation, symptoms of mucositis, and frequent complications of chemotherapy. This study aimed to characterize the chemical profile of the hydroethanolic extract of F. chica and to assess its intestinal anti-inflammatory activity. The chemical profile of the leaves was determined by ultra-performance liquid chromatography coupled to mass spectrometry, and its potential anti-inflammatory activity in the gut was evaluated in mucositis induced by 5-fluorouracil. Three novel compounds from this the species were identified 6,7,3′,4′-tetrahydroxy-5-methoxyflavilium-O-glucuronide, scutellarein-O-glucuronide, and 5-methyl-scutellarein-O-glucuronide, as well as flavones and anthocyanidins that have been previously described. Mice received the hydroethanolic extract (300 mg/kg) for 9 days, and no signs of toxicity were observed. After 72 h of 5-fluorouracil administration, intestinal permeability, bacterial translocation, myeloperoxidase activity, eosinophil peroxidase activity, and histological analyses were performed. Treatment with the analyzed extract was beneficial, as it normalized intestinal permeability, bacterial translocation, myeloperoxidase activity/eosinophil peroxidase and preserved intestinal epithelium architecture. This study provides new insights into the chemical composition and biological activity of the polar extracts from "pariri", an important Amazonian crude medicinal drug.

Cytoprotective, Antiproliferative, and Anti-Oxidant Potential of the Hydroethanolic Extract of Fridericia chica Leaves on Human Cancer Cell Lines Exposed to α- and β-Zearalenol

“…In addition to the properties mentioned, it should be noted that other authors have demonstrated the safety of the extract in an in vivo model with rats, where a dose greater than 200 mg/kg did not induce changes in biochemical and hematological parameters [ 47 , 48 ]. Similarly, Takenaka et al [ 49 ] did not observe signs of toxicity after the treatment with 300 mg/Kg of hydroethanolic extract of F. chica in BALB/c mice.…”

A Review of the Phytochemistry and Pharmacological Properties of the Genus Arrabidaea

“…The intestinal anti-inflammatory activity of HEFc was investigated. The group, which was treated with the plant extract (5-fluorouracil), exhibited partially preserved villi and crypts, reduced infiltration, and preserved muscle layers; the experimental intestinal mucositis model exhibited reduced weight changes [34].…”

“…The guided fractionation of the hydroalcoholic A. chica extract (ACE) facilitated the isolation of four 3-deoxyanthocyanidins, namely 6,7-dihydroxy-5,4-dimethoxy-flavylium (33a, carajurin), 6,7,4 -trihydroxy-5-methoxy-flavylium (33b, carajurone), 6,7,3 ,4 -tetrahydroxy-5methoxy-flavylium (33c), and 6,7,3 -trihydroxy-5,4'-dimethoxy-flavylium (33d) [8] (Table 6). In addition, a new flavone, 6,7,3 ,4 -tetrahydroxy-5-methoxy flavone (34), which is called carajuflavone, three flavonoids, vicenin-2 (35), kaempferol (36), and 4 -hydroxy-3,7dimethoxyflavone (37), were reported for the first time in the genus Arrabidaea [38,39]. The chemical profile of the leaves allowed the isolation and identification of scutellarin (38a) and a rich fraction of the compound (33b), 6,7-trihydroxy-5-methoxyflavone (39), β-carotene (40a), α-carotene (40b), 6,7,3 ,4 -tetrahydroxy-5-methoxyflavilium-O-glucuronide(41a), scutellarein-O-glucuronide (41b), and 5-methyl-scutellarein-O-glucuronide (41c) [34,36,[40][41][42].…”

“…In addition, a new flavone, 6,7,3 ,4 -tetrahydroxy-5-methoxy flavone (34), which is called carajuflavone, three flavonoids, vicenin-2 (35), kaempferol (36), and 4 -hydroxy-3,7dimethoxyflavone (37), were reported for the first time in the genus Arrabidaea [38,39]. The chemical profile of the leaves allowed the isolation and identification of scutellarin (38a) and a rich fraction of the compound (33b), 6,7-trihydroxy-5-methoxyflavone (39), β-carotene (40a), α-carotene (40b), 6,7,3 ,4 -tetrahydroxy-5-methoxyflavilium-O-glucuronide(41a), scutellarein-O-glucuronide (41b), and 5-methyl-scutellarein-O-glucuronide (41c) [34,36,[40][41][42]. The compounds ( 26), (19a) and ( 25) have also been found in A. chica.…”

Therapeutic Potential of Leaves from Fridericia chica (Bonpl.) L. G. Lohmann: Botanical Aspects, Phytochemical and Biological, Anti-Inflammatory, Antioxidant and Healing Action

“…The effectiveness of Fridericia chica leaves as an anti-inflammatory agent has been demonstrated in other models, such as inflammatory angiogenesis, which was induced in a murine model by a sponge implant (polyether-polyurethane) and in the proliferative process of human tumor cell lines in vitro, in which Michel et al (2015) [ 130 ] observed a reduction in the amount of neutrophils at the site of the sponge implants and a great decrease in angiogenesis after treatment with aqueous and ethanolic extracts. Flavone escutelarin derivatives, found in the hydroethanolic extract of Fridericia chica leaves, have been associated with a reduction of parameters linked to inflammatory response in the model of intestinal mucositis induced by 5-fluorouracil in a study by [ 136 ].…”