Chemical Characteristics and In vitro Antidiabetic and Antioxidant Activities of Premna serratifolia L. Leaf Infusion and Decoction

Abstract

Introduction: Leaves of Premna serratifolia L. (local name: arogo) is well known as food ingredient for fish/meat-based soup in Tentena, Indonesia. Evaluation of its bioactivities is needed. Objective: This study aimed to evaluate the α-glucosidase inhibitory and antioxidant activities of infusion and decoction of P. serratifolia leaves. Methods: The leaf samples were prepared by infusion and decoction and analysed for their α-glucosidase inhibitory and antioxidant activities, as well as total phenolic contents and LC-MS profiles. Results: The total phenolic content of the decoction was higher than the infusion. The UV spectra of both infusion and decoction showed peak at 325 nm and shoulder at 285 nm. Their LC-MS yielded one dominant component at 132 (100) m/z fragment, which was tentatively identified as caffeic acid. Both infusion and decoction extracts exhibited strong α-glucosidase inhibition with IC 50 of 4.27 and 0.046 µg GAE/ml, respectively. The IC 50 for DPPH scavenging activity of the infusion and decoction were similar; 6.82 and 7.28 μg GAE/ml, respectively. But the IC 50 of the infusion and decoction for ferrous ion chelating activities were different; 161.66 and 28.34 μg GAE/ ml, respectively. The α-glucosidase inhibitory and antioxidant activities were related to the phenolic content. Conclusion: The study concludes that both infusion and decoction of arogo leaves have antidiabetic effect and potential antioxidant activity. The decoction of the leaves may increase its antidiabetic capacity.

Antioxidant Activity, Enzyme Inhibition Potentials, and Phytochemical Profiling of Premna serratifolia L. Leaf Extracts

“…This study demonstrated that P. serratifolia leaves are inhibitors of α -glucosidase and α -amylase. This observation is in agreement with our previous study, in which it was shown that water extract inhibited α -glucosidase in a competitive mode [ 14 ]. On a previous report, the leaves of P. serratifolia have been reported to have hypoglycemic activity using animal model [ 37 ].…”

“…Stronger activity was observed for EEPS than WEPS ( p < 0.05). Scavenging activities found in these extracts are in agreement with previous studies [ 14 , 26 ]. Previously, extract of other part of P. serratifolia has been reported to have good activity on DPPH radicals.…”

“…Only few papers have been published regarding the pharmacological properties of the leaves of P. serratifolia , such as tumor cell suppression activity [ 10 ], cytotoxic activities on neuroblastoma and melanoma cell lines [ 11 ], and anti-inflammatory and anticancer activities using animal model [ 12 , 13 ]. Recently, we reported antidiabetic and antioxidant properties of the water extract of P. serratifolia leaves [ 14 ]. The present study aimed at extending this investigation on the antioxidant (reducing power and DNA protective) activities and enzyme ( α -glucosidase, α -amylase, xanthine oxidase, and protease) inhibitory properties, of the water and ethanol extracts.…”

α-Glucosidase Inhibitory Effect of Fermented Fruit Juice of Morinda Citrifolia L and Combination Effect with Acarbose

“…α-Glucosidase Assay α-Glucosidase inhibitory activities of fFJ of M.citrifolia was determined based on our reported procedure. 13 Different concentrations of fFJ were prepared by serial dilutions. The reaction mixture was prepared consisting of the sample (50 μl), 50 μl of phosphate buffer (50 mM, pH 6.8) and 50 μl of α-glucosidase (0.5 U/ml).…”

Antidiabetic, Antibacterial and Antioxidant Activities of Different Extracts from Brucea javanica (L.) Merr Seeds

“…The inhibition mode of the most active extract was determined by performing an enzyme kinetic assay based on previous method. 22 In this assay, substrate (p-nitrophenyl α-D-glucopyranoside) was prepared at increasing concentrations (0.25 -1.25 mM). The solution was incubated with α-glucosidase (0.5 U/mL) in the absence and presence of extract (inhibitor) at different concentrations in phosphate buffer (50 mM pH 6.8).…”