Chemical changes during formation and maturation of human deciduous enamel

Robinson, C.; Briggs, H.D.; Atkinson, Peter J.; Weatherell, J.A.

doi:10.1016/0003-9969(81)90113-8

Cited by 43 publications

(27 citation statements)

References 17 publications

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“…Although several biochemical and immunochemical reports have dealt with enamel proteins of the human tooth germ (FINCHAM, 1980;ROBINSON et al, 1981;DEUTSCH et al, 1983DEUTSCH et al, , 1984FINCHAM et al, 1983;ZEICHNER-DAVID et al, 1987), no report has discussed the immunocytochemical localization of enamel proteins, except for a brief description by DEUTSCH (1989) at the light microscopic level. In the present study, we investigated the ultrastructural localization of amelogenin-like immunoreactivity in the deciduous tooth germ of the human fetus.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical localization of amelogenins in the deciduous tooth germs of the human fetus.

Uchida

Tanabe

Fukae

1989

Archives of Histology and Cytology

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Section: Discussionmentioning

confidence: 99%

Immunocytochemical localization of amelogenins in the deciduous tooth germs of the human fetus.

Uchida

Tanabe

Fukae

1989

Archives of Histology and Cytology

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“…Massive net selective 1088 of enamel ~trix proteins' occurs at a falrly_specific stage of deve1op-ment at or shortly after the full thickness of the tissue bas been laid down (Robinson et al, 1971(Robinson et al, , 1978(Robinson et al, , 1981 can be disturbed by nearby turbulence such as that caused by the drill ! .…”

Section: Response Of the Enamel Organ And Ename! To Drilling Proceduresmentioning

confidence: 99%

In vivo experimentation on rat incisor enamel organs through a surgical window

McKee

Warshawsky

1984

Anat. Rec.

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Experimental agents administered systemically are costly and often toxic to animals. An in vivo technique has been developed whereby a surgical window in the alveolar bone allows selected areas of the rat incisor enamel organ and underlying enamel to be exposed to various drugs, radiolabeled molecules, and molecular weight markers. Sherman rats weighing 100 gm were anesthetized and the inferior surface of each hemimandible was surgically exposed. A slow‐speed dental hand drill was used to drill a small hole through the alveolar bone overlying the secretion or maturation zones of the enamel organ. The wound was closed and during recovery the mechanical trauma to the underlying tissue moved away from the hole due to the continuous eruption of the tooth. Two to 5 days later the hole was reexposed and microinjections of 3H‐proline, 125I‐salmon calcitonin, vinblastine sulphate, and normal saline (as control) were administered through the hole with a micro‐manipulator and a microliter syringe. Radioautographic detection of 3H‐proline incorporation in secretory ameloblasts and enamel at 10 minutes, 30 minutes, 1 hour, 4 hours, 1 day, and 2 days after microinjection was identical to that obtained previously by systemic injection. Two hours after microinjection of vinblastine sulphate the cellular response was again identical to that following systemic injection; 125I‐salmon calcitonin (M.W. ∼ 3,600D) was used as a molecular weight marker and was seen to diffuse into the enamel of the maturation zone at 10 minutes after microinjection. This study has demonstrated the feasibility of this new technique for experimentation on rat incisor enamel organs.

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“…At this late stage, full tissue thickness has been achieved; the supporting extracellular matrix largely replaced by fluid and considerable growth in crystal thickness begins. Selective uptake may thus be due to the highly porous, hydrated nature of this developmental stage [Hiller et al, 1975;Robinson et al, 1981Robinson et al, , 1988. Substantial amounts of fluoride are then lost during subsequent maturation.…”

Section: Stage Of Fluoride Uptakementioning

confidence: 99%

The Effect of Fluoride on the Developing Tooth

et al. 2004

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This review aims to outline the effects of fluoride on the biological processes involved in the formation of tooth tissues, particularly dental enamel. Attention has been focused on mechanisms which, if compromised, could give rise to dental fluorosis. The literature is extensive and often confusing but a much clearer picture is emerging based on recent more detailed knowledge of odontogenesis. Opacity, characteristic of fluorotic enamel, results from incomplete apatite crystal growth. How this occurs is suggested by other changes brought about by fluoride. Matrix proteins, associated with the mineral phase, normally degraded and removed to permit final crystal growth, are to some extent retained in fluorotic tissue. Fluoride and magnesium concentrations increase while carbonate is reduced. Crystal surface morphology at the nano-scale is altered and functional ameloblast morphology at the maturation stage also changes. Fluoride incorporation into enamel apatite produces more stable crystals. Local supersaturation levels with regard to the fluoridated mineral will also be elevated facilitating crystal growth. Such changes in crystal chemistry and morphology, involving stronger ionic and hydrogen bonds, also lead to greater binding of modulating matrix proteins and proteolytic enzymes. This results in reduced degradation and enhanced retention of protein components in mature tissue. This is most likely responsible for porous fluorotic tissue, since matrix protein removal is necessary for unimpaired crystal growth. To resolve the outstanding problems of the role of cell changes and the precise reasons for protein retention more detailed studies will be required of alterations to cell function, effect on specific protein species and the nano-chemistry of the apatite crystal surfaces.

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Chemical changes during formation and maturation of human deciduous enamel

Cited by 43 publications

References 17 publications

Immunocytochemical localization of amelogenins in the deciduous tooth germs of the human fetus.

Immunocytochemical localization of amelogenins in the deciduous tooth germs of the human fetus.

In vivo experimentation on rat incisor enamel organs through a surgical window

The Effect of Fluoride on the Developing Tooth

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