Immunocytochemical localization of amelogenins in the deciduous tooth germs of the human fetus.

Uchida, Takashi; Tanabe, T.; Fukae, Makoto

doi:10.1679/aohc.52.543

Cited by 40 publications

(33 citation statements)

References 28 publications

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“…The data obtained show that tooth samples can be successfully cryostabilized and revealed no major differences in cell architecture, intracellular organization, and distribution of enamel proteins when using rapid freezing or chemical fixation followed by freeze-substitution, as compared to conventional tissue processing (for comparative examples of conventionally processed ameloblasts see Kallenbach et al, 1963;Warshawsky and Moore, 1967;Frank and Nalbandian, 1967;Reith, 1970;Smith, 1979;Ozawa et al, 1983;Nanci and Warshawsky, 1984;Nanci et al, 1987a;Uchida et al, 1989;Sasaki, 1990). Indeed, early studies by Moor et al, tion view of nmcloblnsts cut in cross-section.…”

Section: Lmmunocytochemical Labeling Discussionmentioning

confidence: 96%

Ultrastructural studies and immunolocalization of enamel proteins in rodent secretory stage ameloblasts processed by various cryofixation methods

1994

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Section: Lmmunocytochemical Labeling Discussionmentioning

confidence: 96%

Ultrastructural studies and immunolocalization of enamel proteins in rodent secretory stage ameloblasts processed by various cryofixation methods

1994

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“…Quantitative analysis of the immunocytochemical labeling confirmed the overall loss of protein known to occur as enamel progresses through the various stages of amelogenesis, and furthermore, clearly established the extent of the surface-to-DEJ (vertical) gradient in enamel. This gradient has similarly been shown using various histological stains on sections of teeth of limited eruption in several different species (Suga, 1960;Frank and Nalbandian, 1967;Kallenbach, 1977;Eisenmann, 1989;Boyde, 1989;McKee et al, 19911, and by a gradient of immunocytochemical labeling on sections of human, pig, and rat teeth (Uchida et al, 1989(Uchida et al, , 1991. Kallenbach (1977) referred to this particular phenomenon as "matrix reduction."…”

Section: Extracellular Distribution Of Enamel Proteinsmentioning

confidence: 70%

“…Such observations are consistent with the recent hypothesis that most of the enamel proteins are secreted vectorially at the apical surface (rod and interrod secretory sites), but some are also released basolaterallv. The latter material is Enamel proteins are immunodetected in lysosomal elements of ameloblasts at all stages of amelogenesis (Inage et al, 1989;Nanci et al, 1989;Uchida et al, 1989;Inai et al, 1991). Although indicating that some then quickly re-internalized by the ameloblasts, probably by fluid phase endocytosis, and cycled through their lysosomal system .…”

Section: Degradation Of Enamel Proteins By Ameloblastsmentioning

confidence: 99%

“…Only recently has it been shown that the inner enamel epithelium becomes differentiated and initiates secretion of proteins (recognized by antibodies to enamel proteins) significantly earlier than previously considered (Slavkin et al, 1988;Inage et al, 1989;Nanci et al, 1989;Uchida et al, 1989;Inai et al, 1991). Early ultrastructural studies described the existence of a granular material of varying texture associated with the distal ends of differentiating ameloblasts, particularly following initiation of dentin mineralization (Watson, 1960;Fearnhead, 1961;Reith, 1967;Kallenbach, 1971).…”

Section: Early Events Related To Enamel Protein Secretion By Ameloblastsmentioning

confidence: 99%

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Immunolocalization of enamel proteins during amelogenesis in the cat

1992

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Amelogenesis in the cat has been suggested to closely resemble enamel formation in human teeth. In order to further characterize the sequence of events leading to enamel formation in the cat, the expression and distribution of enamel proteins throughout amelogenesis were examined by postembedding immunocytochemistry using an antibody to mouse amelogenins and the high resolution protein A-gold technique. Enamel proteins were first immunodetected in ameloblasts and in the extracellular matrix during the presecretory stage. Secretory stage ameloblasts showed the most intense cellular reactivity. In these cells, protein synthetic organelles, secretory granules, and large lysosome-like structures were all intensely labeled. Extracellularly, numerous gold particles were observed over enamel and over patches of material found at the baso-lateral surfaces of these ameloblasts. During the early maturation stage, the protein synthetic organelles and secretory granules of ameloblasts still showed some immunoreactivity, although the most conspicuous labeling at this later stage was found over enamel and over material present among the extensive apical membrane infoldings of ruffle-ended ameloblasts. Qualitative analysis of lysosome-like elements in ameloblasts suggested that their frequency and immunoreactivity in the maturation stage were relatively lower than in the secretory stage, where some groups of cells often showed numerous large labeled structures. The enamel matrix was intensely labeled at all stages; however, cervical-occlusal and surface-depth gradients were readily apparent by conventional staining and by quantitative analysis of immunolabeling in the late secretory and early maturation stages. These data suggest that the cellular and extracellular distribution of enamel proteins in the cat is generally similar to that reported in other species, although some particularities were observed, perhaps reflecting variation in the timing of developmental parameters.

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“…Por lo tanto, constituirían células odontogénicas epiteliales con diferenciación ameloblástica que producen material tipo matriz de esmalte acumulado en su citoplasma durante el proceso patológico. En trabajos experimentales aplicando técnicas de inmunohistoquímica durante la organogénesis dentaria, se demostró que la amelogenina es segregada en etapas tardías de la diferenciación de los preameloblastos y en etapas iniciales de diferenciación de los ameloblastos secretores (20). En estadios iniciales de la amelogénesis el esmalte en desarrollo se tiñe de rojo intenso con Tricrómico de Masson.…”

Section: Discussionunclassified

Tumores odontogénicos a células fantasmas: Conceptos actuales y aporte de 10 nuevos casos

Basmayean¹,

Moiana²,

Olivera³

2013

Av Odontoestomatol

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RESUMENSe presentan 10 nuevos casos de neoplasias odontogénicas a células fantasmas del archivo de la Cátedra de Anatomía Patológica de la Facultad de Odontología de la UDELAR de Montevideo, Uruguay. Estos tumores constituyeron el 0,08% del total de biopsias del Servicio y el 2,9% de todas las neoplasias odontogénicas registradas. Nueve fueron tumores odontogénicos quísticos calcificantes y uno fue tumor dentinogénico a células fantasmas. Ocho casos fueron centrales y dos periféricos (uno, quístico y el otro, sólido). Se registraron las características clínicas, radiográficas e histopatológicas y se compararon con otros trabajos de la literatura internacional.Palabras clave: Tumores odontogénicos, tumores odontogénicos a células fantasmas, quiste odontogénico calcificante, tumor odontogénico quístico calcificante, tumor dentinogénico de células fantasmas. SUMMARYThis paper reports 10 new cases of ghost cell odontogenic tumors retrieved from the files of the Oral Pathology Service of the Faculty of Dentistry (UDELAR), Montevideo, Uruguay. These tumors accounted for 0.08% of total biopsies and 2.9 % of all registered odontogenic neoplasms. Nine cases were calcifying cystic odontogenic tumors (CCOT) and one dentinogenic ghost cell tumor (DGCT), eight in central and two in peripheral locations (one cystic and one solid variant). Clinical, radiographic and histopathological features are presented and compared with reported cases in literature.

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Immunocytochemical localization of amelogenins in the deciduous tooth germs of the human fetus.

Cited by 40 publications

References 28 publications

Ultrastructural studies and immunolocalization of enamel proteins in rodent secretory stage ameloblasts processed by various cryofixation methods

Ultrastructural studies and immunolocalization of enamel proteins in rodent secretory stage ameloblasts processed by various cryofixation methods

Immunolocalization of enamel proteins during amelogenesis in the cat

Tumores odontogénicos a células fantasmas: Conceptos actuales y aporte de 10 nuevos casos

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