Chemical castration using an intratesticular injection of mannitol: a preliminary study in a rat model

MAADI, Mohammad-Ali; Behfar, Mehdi; RASAEI, Amir; Jalali, Ali Shalizar; Najafi, Gholamreza; Mohammadi, Vahid

doi:10.3906/vet-2010-111

Cited by 3 publications

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“…Our study found no observed side effects in the animals after intratesticular HM and HS in donkey testis. Similar to our finding, a previous study previous study found that side effects such as diarrhea, lethargy, vomiting, scrotal ulceration, and dermatitis were not observed following intratesticular HM injection [ 29 ].…”

Section: Discussionsupporting

confidence: 92%

“…8 ). In mammals, intratesticular injection of calcium chloride, zinc gluconate, HM, and HS have all been tested for chemical castration [ 3 , 16 , 17 , 29 ]. Animals have been reported to be chemically, surgically and mechanically castrated [ 13 , 59 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

“…Fifteen adult male donkeys were randomly assigned to one of five treatment groups: (1) Control group (no treatment), (2) Surgery group (surgical castration for testosterone control), (3) NS group (receiving a single bilateral intratesticular injection (20 mL per testis) of normal saline (sodium chloride 0.9%, Iran Injection and Pharmaceutical Products Company, Tehran, Iran), (4) HS group (receiving a single bilateral intratesticular injection of sodium chloride 20%, Iran Injection and Pharmaceutical Products Company, Tehran, Iran), and (5) HM group (receiving a single bilateral intratesticular injection of HM 20% (Iran injection and pharmaceutical products company, Tehran, Iran) (Fig. 1 ), [ 3 , 29 ].

Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Administration of hypertonic saline can induce acute local osmotic shock and result in widespread necrosis. Also, this approach was effective in sterilization in adult canine and rat models [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…HM, a sugar alcohol, is incompletely metabolized and is excreted in its native form through the urinary tract. Maadi et al (2021) demonstrated that single intratesticular injection of HM resulted in sterilizations in rats [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Baqerkhani,

Soleimanzadeh,

Mohammadi

2024

BMC Vet Res

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Objectives The aim of the present study was to examine donkey sperm quality after intratesticular injection of hypertonic mannitol (HM) and saline (HS). Methods Randomly assigned to five treatment groups were 15 adult male donkeys: (1) Control group (no treatment), (2) Surgery group (surgical castration for testosterone control), (3) NS group (normal saline intratesticular injection), (4) HS group (hypertonic saline), and (5) HM group. We injected 20 mL per testicle. We took 5 mL blood from all donkeys before injection. Castration was performed under general anesthesia 60 days later. Samples included blood and testicular tissue. Total motility (TM), progressive motility (PM), movementy features, DNA damage, morphology, viability, and plasma membrane functionality were evaluated. Hormone analyses, histomorphometric studies and oxidative stress indices including total antioxidant capacity (TAC), glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), and NADP+/NADPH were evaluated. Apoptosis, pyroptosis-related Bax, Caspase-1, GSDMD, and Bcl-2 expression were also assessed. Results In HS and HM groups, testosterone, epididymal sperm count, motility, viability, and plasma membrane functionality dropped while sperm DNA damage increased. HS and HM groups had significantly lower histomorphometric parameters, TAC, GPx, SOD, GSH, and Bcl-2 gene expression. MDA, NADP+/NADPH, Bax, Caspase-1, and GSDMD gene expression were substantially higher in the HS and HM groups than in the control group. Conclusions Toxic effects of hypertonic saline and mannitol on reproductive parameters were seen following, hence, they might be considered as a good chemical sterilizing treatment in donkeys.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Fig.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Baqerkhani,

Soleimanzadeh,

Mohammadi

2024

BMC Vet Res

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Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications

Coimbra

Dantas

Andrade³

et al. 2023

Toxicology

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Male animal sterilization: history, current practices, and potential methods for replacing castration

Hess,

Park,

Soto

et al. 2024

Front. Vet. Sci.

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Sterilization and castration have been synonyms for thousands of years. Making an animal sterile meant to render them incapable of producing offspring. Castration or the physical removal of the testes was discovered to be the most simple but reliable method for managing reproduction and sexual behavior in the male. Today, there continues to be global utilization of castration in domestic animals. More than six hundred million pigs are castrated every year, and surgical removal of testes in dogs and cats is a routine practice in veterinary medicine. However, modern biological research has extended the meaning of sterilization to include methods that spare testis removal and involve a variety of options, from chemical castration and immunocastration to various methods of vasectomy. This review begins with the history of sterilization, showing a direct link between its practice in man and animals. Then, it traces the evolution of concepts for inducing sterility, where research has overlapped with basic studies of reproductive hormones and the discovery of testicular toxicants, some of which serve as sterilizing agents in rodent pests. Finally, the most recent efforts to use the immune system and gene editing to block hormonal stimulation of testis function are discussed. As we respond to the crisis of animal overpopulation and strive for better animal welfare, these novel methods provide optimism for replacing surgical castration in some species.

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Chemical castration using an intratesticular injection of mannitol: a preliminary study in a rat model

Cited by 3 publications

References 0 publications

Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications

Male animal sterilization: history, current practices, and potential methods for replacing castration

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