Chemical Assay of Gonadotrophin in Urine

Crooke, A. C.; Butt, W. R.; Ingram, J.N.; Romanchuck, LudmilaE.

doi:10.1016/s0140-6736(54)90925-3

Cited by 14 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The method of column chromatography for the preparation of GA produces a sub¬ stance which contains fewer inactive proteins, and is probably of more constant composition, than those preparations described here [Butt & Crooke, 1953;Crooke et al 1954]. It was demonstrated by electrophoresis on paper over a wide range of pH that GA so prepared migrated to produce a sharp band [Butt & Dériaz, 1953], whereas the material described here produced a diffuse band.…”

Section: Discussionmentioning

confidence: 86%

“…Glucosamine was measured by a modification [Rimington, 1940] of the method of Elson & Morgan [1933], and fucose by the method of Dische & Shettles [1948] after hydrolysis in 2n-H2S04 for 4 hr at 105°C. The preparations were examined by polarography in the presence of cobalt nitrate [Crooke et al 1954], and cysteine was measured by the same method [Brdîcka, 1933] after acid hydrolysis.…”

Section: Sources Of Materialsmentioning

confidence: 99%

“…By adsorption of gonadotrophin on kaolin followed by elution in 0-lN-NaOH, precipitation from acetone and washing with ether [Dekanski, 1949;Crooke et al 1954].…”

Section: Experimental Procedures and Findingsmentioning

confidence: 99%

“…The chemical measurement of GA, particularly from the urine of women during the reproductive years, was used as an index of gonadotrophic function [Crooke, Butt, Ingram & Romanchuck, 1954]. The amount excreted, when measured by reaction with ninhydrin, fluctuated in a cyclic manner during the menstrual cycle, but the results could not be evaluated by bioassay because of the insensitivity of the biological methods.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Studies on Urinary Gonadotrophins After Fractionation by Tricalcium Phosphate

Butt¹

1956

Journal of Endocrinology

View full text Add to dashboard Cite

1. The chemical properties of a gonadotrophic substance, gonadotrophin A (GA), prepared from human urine, have been studied.2. Preparations of GA from various sources showed marked differences in biological activity, but not in chemical properties. Highly active preparations, however, showed greater u.v. absorption between the wavelengths of 280 and 295 m\g=m\.3. Methods are described for the purification of GA by countercurrent distribution and partition chromatography.The preparation of a gonadotrophic substance, gonadotrophin A (GA), from the urine of postmenopausal women was described by Butt & Crooke [1953]. The chemical measurement of GA, particularly from the urine of women during the reproductive years, was used as an index of gonadotrophic function [Crooke, Butt, Ingram & Romanchuck, 1954]. The amount excreted, when measured by reaction with ninhydrin, fluctuated in a cyclic manner during the menstrual cycle, but the results could not be evaluated by bioassay because of the insensitivity of the biological methods. Large stocks of GA from various sources were therefore prepared so that the chemical results could be checked by accurate bioassays. Brown [1956] has thus shown, by using GA from the urine of postmenopausal women as a reference sub¬ stance, that the activity of similar preparations from younger women is much less than the chemical results indicated.The object of this investigation has been to determine whether there are chemical differences in GA, prepared from various sources, which account for the variations in biological activity. A preliminary report was given by Butt & Dériaz [1953]. MATERIALS AND METHODS Sources of materialsLarge stocks of GA were prepared from pooled urine collected from postmenopausal women, healthy children between the ages of 2 and 4 years, a woman aged 65 with Simmonds' disease following surgical removal of a chromophobe adenoma of the pituitary gland, and a man aged 33 with panhypopituitarism. A sample of gonado¬ trophin from pooled urine of postmenopausal women was obtained from Organon Ltd. Smaller quantities of GA were obtained from 24 hr specimens of urine collected from normal and diseased persons.

show abstract

Section: Discussionmentioning

confidence: 86%

Section: Sources Of Materialsmentioning

confidence: 99%

“…By adsorption of gonadotrophin on kaolin followed by elution in 0-lN-NaOH, precipitation from acetone and washing with ether [Dekanski, 1949;Crooke et al 1954].…”

Section: Experimental Procedures and Findingsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Studies on Urinary Gonadotrophins After Fractionation by Tricalcium Phosphate

Butt¹

1956

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Both materials were prepared from urinary extracts obtained by the same method as that used in the main investigation. In addition they were treated with calcium phosphate by the short method of Crooke, Butt, Ingram & Romanchuck(1954), the material not adsorbed being precipitated as the substandard.…”

Section: Calibration Of Substandardsmentioning

confidence: 99%

Urinary Gonadotrophins in Post-Menopausal Women

Brown¹

1963

Journal of Endocrinology

View full text Add to dashboard Cite

Urinary gonadotrophins from post-menopausal patients were tested by two methods of bioassay. Measured by the response of the mouse uterus, the daily excretion of total gonadotrophin in the less severely disabled patients ranged from 10 to 200 mg. equivalents of HMG 20 A. Patients with active rheumatoid arthritis showed similar levels but some patients with severe disability due to anorexia, reticulosis or anaemia excreted very little gonadotrophin.The gonadotrophins were also measured in terms of follicle-stimulating hormone (FSH) using the ovarian response in mice treated with excess human chorionic gonadotrophin. It was found that they differed strikingly from HMG 20 A in being relatively more potent in the assay for FSH. This suggests that they contained relatively more FSH.The day-to-day fluctuation in the excretion of total gonadotrophin was compared with the differences between duplicate estimations. There were considerable quantitative fluctuations which could not be explained by the experimental errors. There was little evidence of major fluctuation in the quality of the gonadotrophins when this was assessed in a similar way.

show abstract