Chemical, Antioxidant and Antimicrobial Investigations of Pinus cembra L. Bark and Needles

Apetrei, Cristina Lungu; Tuchiluş, Cristina; Aprotosoaie, Ana Clara; Oprea, Adrian; Malterud, Karl Egil; Miron, Anca

doi:10.3390/molecules16097773

Cited by 75 publications

(62 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the presence of the electron-donating compounds, the potassium ferric/ferricyanide complex is reduced to its ferrous form (Fe 2+ ). The amount of Fe 2+ is then quantitatively monitored by measuring the intensity of the Perls Prussian blue colour complex formed by reaction with ferric chloride, at 700 nm [30]. The reaction between the ferrous form and the ferric chloride is:…”

Section: Ferric Reducing Powermentioning

confidence: 99%

“…The ferric reducing power of the compounds was quantified by the method described by [30] with slight modifications. phosphate buffer (1 mL, 0.2 M, pH 6.6) and potassium ferricyanide (1 mL, 1% w/v) were added in a test tube to the sample (50 µL, 100 µL, 200 µL, 300 µL) obtained from a stock solution (5 mg/mL in DMSO).…”

Section: Ferric Reducing Powermentioning

confidence: 99%

“…The method is based on the reduction of Mo (VI) to Mo (V) by the tested compounds followed by the formation of a green phosphate/Mo(V) complex at acid pH. There were used different sample volumes (10,15,20,30,40, 60 µL) obtained from a stock solution (5 mg/mL in DMSO). The samples were mixed with the reagent solution (2 mL, 28 mM sodium phosphate; 4 mM ammonium molybdate; 0.6 M sulphuric acid), incubated at 95 °C for 90 min, cooled at room temperature and then centrifuged at 4,500 rpm for 15 min.…”

Section: Total Antioxidant Activitymentioning

confidence: 99%

See 2 more Smart Citations

The Synthesis and the Biological Evaluation of New Thiazolidin-4-one Derivatives Containing a Xanthine Moiety

et al. 2013

View full text Add to dashboard Cite

Abstract:Starting from theophylline (1,3-dimethylxanthine) new thiazolidin-4-one derivatives 7a 1-7 , 7b 1-7 have been synthesized as potential antidiabetic drugs. The structure of the new derivatives was confirmed using spectral methods (FT-IR, 1 H-NMR, 13 C-NMR). The in vitro antioxidant potential of the synthesized compounds was evaluated according to the ferric reducing power, the total antioxidant activity and the DPPH and ABTS radical scavenging assays. Reactive oxygen species (ROS) and free radicals are considered to be implicated in a variety of pathological events, such as diabetes mellitus and its micro-and macrovascular complications. The results of chemical modulation of the thiazolidin-4-one intermediaries 6a, 6b through condensation with several aromatic aldehydes is the improvement of the antioxidant effect. All benzylidenethiazolidin-4-one derivatives 7a 1-7 , 7b 1-7 are more active than their parent thiazolidin-4-ones. The most active compounds are the ones obtained by reaction of condensation with 4-hydroxybenzaldehyde (compounds 7a 5 , 7a 6 ), 4-dimethylaminobenzaldehyde (compounds 7a 6 , 7b 6 ) and 2-nitrobenzaldehyde (compounds 7a 7 , 7b 7 ).

show abstract

Section: Ferric Reducing Powermentioning

confidence: 99%

Section: Ferric Reducing Powermentioning

confidence: 99%

Section: Total Antioxidant Activitymentioning

confidence: 99%

See 1 more Smart Citation

The Synthesis and the Biological Evaluation of New Thiazolidin-4-one Derivatives Containing a Xanthine Moiety

et al. 2013

View full text Add to dashboard Cite

show abstract

“…While the developed antioxidant sensor was characterized with GA, the obtained antioxidant capacity of the tested herbal extracts can be validated by comparing with the antioxidant activity of GA since this approach was done in many studies [47][48][49][50]. Using the standard calibration curve of GA obtained by scanometry technique, the antioxidant capacity of each extract was calculated and expressed as gallic acid equivalent in mg/L (mg/L GAE) as described in our previous work [29].…”

Section: Application Of Antioxidant Sensor On the Plant Extractsmentioning

confidence: 99%

Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

Kuswandi¹,

Sari²,

Hidayat³

2018

jrp

View full text Add to dashboard Cite

How to cite this article: Hidayat MA, Sari P, Kuswandi B. Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts. Marmara Pharm J. ABSTRACT: A simple scanometric assay for antioxidant capacity of the herbal extracts has been developed based on dry reagent of 2,2-diphenyl-1-picrylhydrazyl (DPPH) immobilized on the pharmaceutical blister. The deep violet color of DPPH solution was regenerated by introducing methanol to each well as sensing zones. The antioxidant capacity of plant extract will proportionally reduce the violet color to form the corresponding yellow color adduct, where the color change was captured by a flatbed scanner. Then, the optical responses toward antioxidant are presented as an averaged value of the red green blue (RGB) color using image color analysis (ImageJ). By using this color value, analytical performance of the antioxidant sensor could be characterized, where it has a linear range between 1-28 mg/L as gallic acid equivalent (GAE), with response time at 10 min. The reproducibility of the antioxidant sensor was good (RSD < 1%) with the recovery value of 102-105%. The scanometric assay was applied to several herbal extracts, such as sappan wood, guava leaf, and turmeric rhizome. The results obtained are in good agreement with the spectrophotometric method, suggesting that the proposed assay can be used as an alternative method for antioxidant capacity determination.

show abstract

“…3,4 Bark extracts from other Pinus species have been reported to possess potent antioxidant activity. [7][8][9][10] Extracts of Pinus sylvestris, Pinus pinea, and Pinus massoniana barks showed cytotoxic activity against human cancer cell lines. 8,9 In addition, P. sylvestris and P. massoniana bark extracts reduced the production of several inflammatory mediators (nitric oxide, prostaglandin E 2 , and intercellular adhesion molecule-1).…”

Section: Introduction Bmentioning

confidence: 99%

In VitroStudy on the Antioxidant Activity of a Polyphenol-Rich Extract fromPinus brutiaBark and Its Fractions

Creţu

Karonen

Salminen

et al. 2013

Journal of Medicinal Food

Self Cite

View full text Add to dashboard Cite

A crude hydromethanolic extract from Pinus brutia bark and its fractions (diethyl ether, ethyl acetate, n-butanol, and aqueous fractions) were studied with regard to their phenolic content and antioxidant activities. The total phenolics and proanthocyanidins in each extract were quantified by spectrophotometric methods; the polyphenolic profile was analyzed by RP-HPLC-DAD-ESI-MS. All extracts were tested with regard to their ability to scavenge free radicals (ABTS radical cation, superoxide and hydroxyl radicals), reduce ferric ions, and inhibit 15-lipoxygenase. P. brutia bark extracts had high phenolic contents (303.79 -7.34-448.90 -1.39 mg/g). Except diethyl ether extract, all other extracts contained proanthocyanidins ranging from 225.79 -3.94 to 250.40 -1.44 mg/g. Several polyphenols were identified by RP-HPLC-DAD-ESI-MS: taxifolin in diethyl ether extract, a taxifolin-O-hexoside, catechin, procyanidin dimers, and trimers in ethyl acetate extract. Except diethyl ether extract, all other extracts were effective scavengers of superoxide and hydroxyl radicals (EC50 = 33.5 -1.1-54.93 -2.85 lg/mL and 0.47 -0.06-0.6 -0.0 mg/mL, respectively). All extracts had noticeable 15-lipoxygenase inhibitory effects (EC50 = 22.47 -0.75-34.43 -2.25 lg/mL). We conclude that P. brutia bark is very promising for the dietary supplements industry due to its high free radical scavenging and 15-lipoxygenase inhibitory effects.

show abstract

Chemical, Antioxidant and Antimicrobial Investigations of Pinus cembra L. Bark and Needles

Cited by 75 publications

References 47 publications

The Synthesis and the Biological Evaluation of New Thiazolidin-4-one Derivatives Containing a Xanthine Moiety

The Synthesis and the Biological Evaluation of New Thiazolidin-4-one Derivatives Containing a Xanthine Moiety

Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

In VitroStudy on the Antioxidant Activity of a Polyphenol-Rich Extract fromPinus brutiaBark and Its Fractions

Contact Info

Product

Resources

About