Chemical and toxicological effects of medicinal Baccharis trimera extract from coal burning area

Menezes, Ana Paula Simões; Silva, Juliana da; Fisher, Camila; Silva, Fernanda R. da; Reyes, Juliana; Picada, Jaqueline Nascimento; Ferraz, Alice Gomes; Corrêa, Dione Silva; Premoli, Suziane; Dias, Johnny Ferraz; Souza, Cláudia Telles de

doi:10.1016/j.chemosphere.2015.12.028

Cited by 25 publications

(13 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…34 , who measured PHA in harp seal beaters ( Phoca groenlandica ) and did not find a difference between males and females. The main cellular mechanism of DNA damage discussed by the authors was the oxidative lesions caused by PHA 9,18,35 .…”

Section: Discussionmentioning

confidence: 99%

“…Coal is a fossil fuel that causes impacts through environmental pollution from its extraction to its combustion. A wide range of coal dust and coal byproducts contribute to the pollution of the atmosphere 8 , water and soil 9,10 . Inorganic elements (metals) and polycyclic aromatic hydrocarbons (PAH), which are found in coal, can induce DNA damage by introducing a cellular oxidative imbalance by the generation of several reactive oxygen species (ROS) 11–13 .…”

Section: Introductionmentioning

confidence: 99%

“…The presence of PAH was found in its coal, bottom and fly ash samples 29 , metals were detected in soil samples 30 and Zn, Ni, Cu, Cd, V and Pb were detected in the tissues of the subterranean rodent C. torquatus 23 . In addition, studies with organisms have shown DNA damage in plants ( Baccharis trimera ) 9,31 , snail ( Helix aspersa ) 10 and coal workers’ blood 30,32,33 . To our knowledge, no previous studies have examined the effects of coal and its byproducts on telomere length or DNA global methylation in tuco-tuco.

Figure 1Geographic distribution of C. torquatus , which coincides with the geographic distribution of coal reserves and power plant Candiota (modified from Fernandes et al .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Anthropogenic Effects on Natural Mammalian Populations: Correlation Between Telomere Length and Coal Exposure

Matzenbacher

Silva

Garcia

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

The Candiota coal mine in Rio Grande do Sul (RS) is one of the largest in Brazil. Coal is a fossil fuel that causes environmental impacts from its extraction to combustion due to the release of different agents, such as polycyclic aromatic hydrocarbons (PAH) and heavy metals. C tenomys torquatus are herbivorous and subterranean rodents that dig tunnels with their paws and teeth and can be exposed to coal through contaminated food. Exposure to pollutants can cause DNA damage and affect different tissues, inducing alterations in the population structure and genetic diversity. Our study aimed to evaluate the effect of exposure to coal and its derivatives on the C. torquatus population and to examine the relationship of coal exposure with variations in absolute telomere length (aTL), global DNA methylation and genotoxicity. Our study showed an inverse correlation between telomere length and coal exposure in addition to an increase in DNA damage. The results indicate that coal and its byproducts can contribute to the alteration of the C. torquatus population structure, as evidenced by a reduction in the number of adults.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Figure 1Geographic distribution of C. torquatus , which coincides with the geographic distribution of coal reserves and power plant Candiota (modified from Fernandes et al .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anthropogenic Effects on Natural Mammalian Populations: Correlation Between Telomere Length and Coal Exposure

Matzenbacher

Silva

Garcia

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…These products were visualized under visible UV light (254 nm) and with natural product reagent. The high-performance liquid chromatography (HPLC) analysis followed the previous work with B. trimera [16]. The correlation of chromatographic peaks with quercetin, luteolin, and cirsimaritin was achieved by comparing experimental retention time with reference standards (Sigma, St Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Rodrigues et al [10] have detected a mutagenic activity in mice treated with B. trimera aqueous extract by the increase of micronucleus frequency in bone marrow. In addition, Nogueira et al [15] and Menezes et al [16] have observed that the aqueous extract of B. trimera induces genotoxic effects to kidney cells in vivo .…”

Section: Introductionmentioning

confidence: 99%

Biotoxicological Analyses of Trimeroside from Baccharis trimera Using a Battery of In Vitro Test Systems

Santos

Silva

Menezes

et al. 2018

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

The use in folk medicine of Baccharis trimera and recent studies on DNA damage by oxidative stress mechanisms have motivated this study. We investigated the biotoxicological effects of trimeroside from this plant. Aqueous extract from aerial parts of B. trimera was fractioned by flash chromatography for further isolation by thin-layer chromatography. The novel nor-monoterpene glycoside, trimeroside, and three flavonoids, cirsimaritin, luteolin and quercetin, were isolated. The genotoxic and mutagenic potential of trimeroside was determined by Salmonella/microsome (TA98 and TA100), comet assay, and cytokinesis-block micronucleus cytome assay (CBMN-cyt) in HepG2 cells. We also screened trimeroside into different human tumoral cell lines by sulforhodamine B (SRB) assay. Mutagenicity was detected in TA100 strain with metabolic activation. Genotoxic effects were not observed in HepG2 by comet assay. However, a decrease in the nuclear index division in the 2.0 mg·mL−1 concentration and an increase of nucleoplasmic bridges in the 1.5 mg·mL−1 concentration were detected by CBMN-cyt assay indicating cytotoxic and mutagenic effects. In SRB assay, trimeroside showed weak antiproliferative activity against the cell lines.

show abstract

Comet assay: an essential tool in toxicological research

2016

View full text Add to dashboard Cite

The comet assay is a versatile, reliable, cost-efficient, and fast technique for detecting DNA damage and repair in any tissue. It is useable in almost any cell type and applicable to both eukaryotic and prokaryotic organisms. Instead of highlighting one of the numerous specific aspects of the comet assay, the present review aims at giving an overview about the evolution of this widely applicable method from the first description by Ostling and Johanson to the OECD Guideline 489 for the in vivo mammalian comet assay. In addition, methodical aspects and the influence of critical steps of the assay as well as the evaluation of results and improvements of the method are reviewed. Methodical aspects regarding oxidative DNA damage and repair are also addressed. An overview about the most recent works and relevant cutting-edge reviews based on the comet assay with special regard to, e.g., clinical applications, nanoparticles or environmental risk assessment concludes this review. Taken together, the presented overview raises expectations to further decades of successful applications and enhancements of this excellent method.

show abstract

Chemical and toxicological effects of medicinal Baccharis trimera extract from coal burning area

Cited by 25 publications

References 56 publications

Anthropogenic Effects on Natural Mammalian Populations: Correlation Between Telomere Length and Coal Exposure

Anthropogenic Effects on Natural Mammalian Populations: Correlation Between Telomere Length and Coal Exposure

Biotoxicological Analyses of Trimeroside from Baccharis trimera Using a Battery of In Vitro Test Systems

Comet assay: an essential tool in toxicological research

Contact Info

Product

Resources

About