Chemical and Molecular Composition of the Chrysalis Reveals Common Chitin-Rich Structural Framework for Monarchs and Swallowtails

Goularte, Nicolette F.; Kallem, Till; Cegelski, Lynette

doi:10.1016/j.jmb.2022.167456

Cited by 6 publications

(4 citation statements)

References 42 publications

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“…Spectra of M. smegmatis mAGP obtained with different CP times exhibited a typical increase and slow decrease in signal intensity for carbons across the spectrum ( Fig. 1E ), similar to our published results with comparable types of lyophilized biological solids, including mixed peptide-glycan composites present in bacterial cell walls, extracellular matrix materials, and butterfly chrysalides [ 35 , 39 , 50 ]. Peak intensities for shift-resolved carbons revealed comparable slopes of decay, revealing similar T 1ρ (H) relaxation for mAGP carbons ( Fig.…”

Section: Resultssupporting

confidence: 88%

CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells

Liu

Brčić

Cassell

et al. 2023

Journal of Magnetic Resonance Open

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Section: Resultssupporting

confidence: 88%

CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells

Liu

Brčić

Cassell

et al. 2023

Journal of Magnetic Resonance Open

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“…In the present study, the process conditions established in the isolation of chitosan from the carapace of M. rosenbergii yielded 23.79% and 20.21% chitin and chitosan, respectively ( Table 4 ). Studies have showed that the raw shell of crustaceans contains chitin, protein, calcium carbonate, phosphate, and other compounds [ 49 , 50 ]. To produce chitosan, inorganic elements were first removed by soaking raw shell in 1 M of HCl in a process called demineralization, followed by deproteinization with 1 M NaOH, and subsequently in 60% NaOH at high temperature to achieve deacetylation [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

Response Surface Methodology (RSM) Approach to Optimization of Coagulation-Flocculation of Aquaculture Wastewater Treatment Using Chitosan from Carapace of Giant Freshwater Prawn Macrobrachium rosenbergii

et al. 2023

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The major sources of waste from aquaculture operations emanates from fish or shellfish processing and wastewater generation. A simple technique called coagulation/flocculation utilizes biowaste from aquaculture to produce chitosan coagulant for wastewater treatment. A chemical method was applied in the present study for chitin and chitosan extraction from carapace of Macrobrachium rosenbergii and subsequent application for removal of turbidity and salinity from shrimp aquaculture wastewater. Box-Behnken in RSM was used to determine the optimum operating conditions of chitosan dosage, pH, and settling time, after which quadratic models were developed and validated. Results show that 80 g of raw powder carapace yielded chitin and chitosan of 23.79% and 20.21%, respectively. The low moisture (0.38%) and ash (12.58%) content were an indication of good quality chitosan, while other properties such as water-binding capacity (WBC), fat-binding capacity (FBC), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscope (SEM) confirmed the structure and the α-group, as well as the rough morphology of chitosan. In addition, the high solubility (71.23%) and DDA (85.20%) suggested good coagulant potentials. It was recorded in this study that 87.67% turbidity was successfully removed at 20 mg/L of chitosan dosage and 6.25 pH after 30 min settling time, while 21.43% salinity was removed at 5 mg/L of chitosan dosage, 7.5pH, and 30 min settling time. Therefore, the process conditions adopted in this study yielded chitosan of good quality, suitable as biopolymer coagulant for aquaculture wastewater treatment.

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“…As such, CPMAS experiments have proven useful, and multiple studies have demonstrated that CPMAS can be used quantitatively for lyophilized biological solids as long as CP kinetics are taken into account. 63,64,[82][83][84] Another solid-state NMR experiment that has been employed to study biofilm EPS is rotational-echo double-resonance (REDOR), which provides quantitative information about dipolar coupling strengths between a pair of heteronuclei. Because dipolar coupling is dependent upon internuclear distance, REDOR is used to determine precise distances.…”

Section: Solid-state Nmr As a Tool To Quantitatively Study Biofilm Ma...mentioning

confidence: 99%

“…However, DPMAS experiments can be prohibitively long because they do not benefit from an increase in 13 C signal from CP from the abundant, high gyromagnetic ratio 1 H nuclei or decreases in relaxation time. As such, CPMAS experiments have proven useful, and multiple studies have demonstrated that CPMAS can be used quantitatively for lyophilized biological solids as long as CP kinetics are taken into account 63,64,82–84 . Another solid‐state NMR experiment that has been employed to study biofilm EPS is rotational‐echo double‐resonance (REDOR), which provides quantitative information about dipolar coupling strengths between a pair of heteronuclei.…”

Section: Solid‐state Nmr As a Tool To Quantitatively Study Biofilm Ma...mentioning

confidence: 99%

Challenges and opportunities in elucidating the structures of biofilm exopolysaccharides: A case study of the Pseudomonas aeruginosa exopolysaccharide called Pel

Amyx‐Sherer,

Reichhardt

2023

Magnetic Reson in Chemistry

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Biofilm formation protects bacteria from antibiotic treatment and host immune responses, making biofilm infections difficult to treat. Within biofilms, bacterial cells are entangled in a self‐produced extracellular matrix that typically includes exopolysaccharides. Molecular‐level descriptions of biofilm matrix components, especially exopolysaccharides, have been challenging to attain due to their complex nature and lack of solubility and crystallinity. Solid‐state nuclear magnetic resonance (NMR) has emerged as a key tool to determine the structure of biofilm matrix exopolysaccharides without degradative sample preparation. In this review, we discuss challenges of studying biofilm matrix exopolysaccharides and opportunities to develop solid‐state NMR approaches to study these generally intractable materials. We specifically highlight investigations of the exopolysaccharide called Pel made by the opportunistic pathogen, Pseudomonas aeruginosa. We provide a roadmap for determining exopolysaccharide structure and discuss future opportunities to study such systems using solid‐state NMR. The strategies discussed for elucidating biofilm exopolysaccharide structure should be broadly applicable to studying the structures of other glycans.

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Chemical and Molecular Composition of the Chrysalis Reveals Common Chitin-Rich Structural Framework for Monarchs and Swallowtails

Cited by 6 publications

References 42 publications

CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells

CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells

Response Surface Methodology (RSM) Approach to Optimization of Coagulation-Flocculation of Aquaculture Wastewater Treatment Using Chitosan from Carapace of Giant Freshwater Prawn Macrobrachium rosenbergii

Challenges and opportunities in elucidating the structures of biofilm exopolysaccharides: A case study of the Pseudomonas aeruginosa exopolysaccharide called Pel

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