Chemical and biophysical properties of gelatins extracted from alkali-pretreated skin of cuttlefish (Sepia officinalis) using pepsin

Jridi, Mourad; Nasri, Rim; Lassoued, Imen; Souissi, Nabil; Mbarek, Aïcha; Barkia, Ahmed; Nasri, Monçef

doi:10.1016/j.foodres.2013.09.026

Cited by 68 publications

(45 citation statements)

References 35 publications

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“…these enzyme activities were similar and eve higher than those obtained by CICAFLORA. This is may be due to the presence of peptides in the gelatin elaborated during the extraction process [15,16]. Similarly, Kim et al [38] reported that antioxidant gelatin peptides may protect living cells against free radical mediated oxidative damage by enhancing cell viability against oxidation-induced cell death.…”

Section: Tissue Antioxidant Enzyme Activities and Mda Contentmentioning

confidence: 99%

“…The observed low antioxidant activity of control gelatin gel (G0) and film (F0) was probably related to the presence of some antioxidant peptides probably elaborated during gelatin extraction process [15,16]. Therefore, gelatin matrix alone could be used in dermal wound healing processes due to its bioactivity, biodegradable and biocompatible properties.…”

Section: In Vitro Antioxidant Activity Of Ahe Csg-gels and Csg-filmsmentioning

confidence: 99%

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Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract

Jridi

Sellimi

Lassoued

et al. 2017

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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The in vivo wound-healing potential of gels and films based on cuttlefish skin gelatin (CSG) incorporated with aqueous henna extract (AHE) (at 50 and 500 µg/ml) was studied.Since wound healing is related to reactive oxygen species (ROS), this study was first conducted to evaluate the antioxidant activity of AHE by DPPH radical-scavenging, β-carotene-linoleate bleaching, and reducing power assays. The major volatile compounds were lawsone, dimethyl sulfoxide, dimethyl sulfide, eugenol and maltol. The finding of the study showed that the incorporation of AHE into gelatin gels and films enhanced the antioxidant activity in a dose-dependent manner. In the wound-healing study, topical application of gelatin hydrogels or films enriched by AHE, on the wound site in a rat model, enhanced significantly wound healing activity and helped to prevent from inflammation damage, when compared to the control and CICAFLORA®-treated groups. Further, all preparations were found to improve the antioxidant status of treated rats as was evidenced by increased antioxidant enzymatic activities and reduced MDA content. The increase in the activities of catalase (CAT), superoxide dismutase (SOD) and gluthatione peroxydase (GPx) in wound tissues revealed that bioactive compounds in AHE, film and gel are able to enhance the healing of wounds by reducing the damage caused by ROS. Wound-healing activity was confirmed by histopathology studies. The obtained results showed that the addition of AHE to the gelatin matrix could improve the wound healing activity.

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Section: Tissue Antioxidant Enzyme Activities and Mda Contentmentioning

confidence: 99%

Section: In Vitro Antioxidant Activity Of Ahe Csg-gels and Csg-filmsmentioning

confidence: 99%

Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract

Jridi

Sellimi

Lassoued

et al. 2017

Colloids and Surfaces A: Physicochemical and Engineering Aspect

Self Cite

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“…Comparison of a continuous 48h pepsin extraction with two sequential 24h pepsin digestion indicated that yields increased with the addition of new pepsin. Addition of fresh pepsin may solubilize additional collagen by replacing pepsin that has undergone autolysis (Funatsu and Tokuyasu, 1959), thereby cleaving additional peptide bonds and resulting in an increase in gelatin extraction within the same extraction time Jridi et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Emulsifying properties The emulsion activity index (EAI) and the emulsion stability index (ESI) of BLG were determined according to the methods of Aewsiri et al (2013) and Jridi et al (2013), respectively. Eight mL of gelatin solutions of 0.5, 1.0 and 2.0% were mixed with 2 mL of sunflower oil by Vortex and homogenized for 1 min at 24, 200 rpm at room temperature.…”

Section: Gelatin Extraction With Pepsinmentioning

confidence: 99%

“…The colour was described using the Commission International de L'eclairage (CIE) coordinates L* (lightness), a* (redness/greenness) and b* (yellowness/blueness). Foaming properties Foam expansion (FE) and foam stability (FS) of BLG solutions were determined according to the method of Jridi et al (2013). Twenty five mL of gelatin solutions of 0.5, 1.0 and 2.0% (w/v) were homogenized at 20, 000 rpm for 1 min at room temperature using an Ultra-Turrax Ika T-18 Basic Ultra Turrax Homogenizer (Cole-Parmer, Canada) to incorporate air.…”

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confidence: 99%

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Extraction and Characterization of Gelatin from Bovine Lung

Roy

Omana

Betti

et al. 2017

FSTR

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Gelatin is extracted from animal tissues using heat usually with low yields, but pepsin may increase high quality gelatin yield per unit of tissue. Gelatin from bovine lungs was extracted using heat and pepsin and the resulting gelatins were characterized. Pepsin increased gelatin yield by about 9-fold that of heat extraction alone. All bovine lung gelatin contained protein as the major proximate component, with little ash and non-detectable fat. Bovine lung gelatin had pH, moisture and protein comparable to or less than that of commercial bovine gelatin and decreased ash. Transmittance of bovine lung gelatin was substantially reduced compared to that of commercial bovine gelatin but had increased water and fat-binding capacity, and comparable or increased gelling and melting temperature.Gel strengths of bovine lung gelatin were comparable to or lower than and foam stability and emulsifying activity were lower than commercial bovine gelatin. Increased imino acid (proline and hydroxyproline) content was associated with increased gelling and melting temperatures and was comparable to commercial bovine gelatin.Heat-extracted bovine lung gelatin contained predominantly collagen γ-chains, β-chains and α-chains (α1(I) and α2(I)), with some low molecular weight peptides, while the pepsin-extracted lung gelatins were characterized by comparatively decreased β-and α-chains and increased low molecular weight peptides. The gel strength of heatextracted bovine lung gelatin was higher than that of pepsin-extracted gelatins, indicating that additional yield was associated with reduced gelatin quality. Bovine lung is a potential source of gelatin for application in diversified industrial fields and use of pepsin is a viable method for extracting additional gelatin after heat extraction of high quality (increased gel strength) gelatin from bovine lung.Keywords: gelatin, bovine lung, extraction, pepsin, emulsifying, foaming IntroductionGelatin is a popular biopolymer used in food, pharmaceutical, cosmetic and photographic applications (Schrieber and Gareis, 2007) because of its unique functional properties (Zhou et al., 2006). Gelatin is derived from collagen, the major protein constituent of connective tissue, through partial hydrolysis using hot water, dilute acid or alkali treatments (Zhang et al., 2009). The global gelatin market volume was approximately 373,000 tons in 2013, with this market volume anticipated to grow by 3.8% every year to an estimated worth of $3,000,000,000 by 2020 (Grand View Research Inc., 2014). Gelatin derived from pig skin has accounted for the highest proportion available for use, followed by that from bovine hides and bovine bones (Karim and Bhat, 2009).Bovine Spongiform Encephalopathy (BSE) has been a concern with regard to the safety of gelatin from bovine sources despite the expectation that the probability of prion infectivity is low given the harsh chemical treatment of the raw material during gelatin production (Baziwane and He, 2003) and the Scientific SteeringCommittee of the European Un...

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Bioactive Peptides from Fish Collagen Byproducts

Nasri

2019

Byproducts From Agriculture and Fisheries

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Chemical and biophysical properties of gelatins extracted from alkali-pretreated skin of cuttlefish (Sepia officinalis) using pepsin

Cited by 68 publications

References 35 publications

Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract

Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract

Extraction and Characterization of Gelatin from Bovine Lung

Bioactive Peptides from Fish Collagen Byproducts

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