Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract

Abstract

The in vivo wound-healing potential of gels and films based on cuttlefish skin gelatin (CSG) incorporated with aqueous henna extract (AHE) (at 50 and 500 µg/ml) was studied.Since wound healing is related to reactive oxygen species (ROS), this study was first conducted to evaluate the antioxidant activity of AHE by DPPH radical-scavenging, β-carotene-linoleate bleaching, and reducing power assays. The major volatile compounds were lawsone, dimethyl sulfoxide, dimethyl sulfide, eugenol and maltol. The finding of the study showed that the incorporation of AHE into gelatin gels and films enhanced the antioxidant activity in a dose-dependent manner. In the wound-healing study, topical application of gelatin hydrogels or films enriched by AHE, on the wound site in a rat model, enhanced significantly wound healing activity and helped to prevent from inflammation damage, when compared to the control and CICAFLORA®-treated groups. Further, all preparations were found to improve the antioxidant status of treated rats as was evidenced by increased antioxidant enzymatic activities and reduced MDA content. The increase in the activities of catalase (CAT), superoxide dismutase (SOD) and gluthatione peroxydase (GPx) in wound tissues revealed that bioactive compounds in AHE, film and gel are able to enhance the healing of wounds by reducing the damage caused by ROS. Wound-healing activity was confirmed by histopathology studies. The obtained results showed that the addition of AHE to the gelatin matrix could improve the wound healing activity.

Evaluation of the Wound Healing Properties of Luteolin Ointments on Excision and Incision Wound Models in Diabetic and Non-Diabetic Rats

“…These steps can be characterized into four highly programmed phases such as hemostasis, inflammation, proliferation and remodeling [2,6,[12][13][14][15][16]. All phases of healing must occur in suitable sequence for successful healing as a natural phenomenon [6,12,13] but sometimes complex multifactorial process of wound healing can be delayed or failed by unwanted effects such as presence of free radicals [12,17] or microbial infections [5,12,16,17].…”

Preparation and In Vitro Biological Evaluation of Lawsone Loaded O‐Carboxymethyl Chitosan/Zinc Oxide Nanocomposite for Wound‐Healing Application

“…The zinc oxide incorporated chitosan/PVA nanofibrous mats shows noted scavenging property . The proton contributing capability of phenolic groups in henna leaves convert free radicals to a stable product and arrest radical chain reaction . Additionally, lawsone supports in modulating redox signalling by different pathways .…”

“…Usually, lawsone has poor solubility, in order to maintain its bioavailability incorporation with bioactive polymers was followed . The histopathological analysis of aqueous henna extract loaded Cuttlefish gelatin film shows pronounced antioxidant and wound healing . It has been reported that the lawsone loaded hydroxyethyl cellulose/PVP hydrogel and PLLA/gelatin nanofiber shows good antibacterial and biocompatibility towards fibroblast cells.…”

Advances and Impact of Antioxidant Hydrogel in Chronic Wound Healing

“…Clinically, chronic wounds usually refer to wounds caused by various reasons, which cannot automatically restore their functional integrity within three months, and also have no tendency to heal . More seriously, due to the influence of potential physiological conditions, such as diabetes, chronic wounds not only heal slowly but are also often prone to recurrence .…”

“…If inflammation is not controlled, it will lead to the self‐secretion of extracellular polysaccharide matrix, which will protect bacteria from the host's immune response and antibiotic therapy . In addition, persistent hyperglycemia would elevate the levels of advanced glycation end products (AGEs) in the blood, which can directly induce high concentration of ROS . This may disrupt the redox balance of the cells, causing metabolic disorders that further impair the structural integrity of the blood vessels and tissue regeneration …”