Chemical and Biological Assessment of Angelicae Sinensis Radix after Processing with Wine: An Orthogonal Array Design To Reveal the Optimized Conditions

Abstract

The roots of Angelica sinensis [Angelica Sinensis Radix (ASR)] have been used as a common health food supplement for women's care for thousands of years in China. According to Asian tradition, ASR could be processed with the treatment of wine, which subsequently promoted the biological functions of ASR. By chemical and biological assessments, an orthogonal array design was employed here to determine the roles of three variable parameters in the processing of ASR, including oven temperature, baking time, and flipping frequency. The results suggested that oven temperature and baking time were two significant factors, while flipping frequency was a subordinate factor. The optimized condition of processing with wine therefore was considered to be heating in an oven at 80 °C for 90 min with flipping twice per hour. Under the optimized processing conditions, the solubilities of ferulic acid and Z-ligustilide from ASR were markedly increased and decreased, respectively. In parallel, the biological functions of processed ASR were enhanced in both anti-platelet aggregation and estrogenic activation; these increased functions could be a result of the altered levels of ferulic acid and Z-ligustilide in wine-processed ASR. Thus, the chemical and biological assessment of the processed ASR was in full accordance with the Chinese old tradition.

The potential of metabolic fingerprinting as a tool for the modernisation of TCM preparations

“…This is of special importance for the detoxification of toxic materials like Aconitum drugs (Singhuber et al, 2010) but also for the preservation of active constituents, ease of administration, flavour correction or cleansing (Zhu, 1998). Many drugs can be processed by various methods like stir-frying, steaming or calcining in order to gain a product with altered therapeutic properties and -implicitly -altered chemical composition (Zhao et al, 2010;Zhan et al, 2011).…”

Simultaneous determination of six hydrophilic components in rat plasma after oral administration of Jitai tablet by liquid chromatography–electrospray ionization–tandem mass spectrometry: Application to a pharmacokinetic study

“…Previous chemical investigations and pharmacological studies also discovered that the water-soluble compounds, including DSS, SAA and SAB are the representative agents of Radix Salviae Miltiorrhiae. Additionally, ADL, FA and HSYA are also the important constituents owing to their antiemetics [10], antiinflammatory [11][12][13], antinociceptive [14,15], anxiolytic [16], and sedative tranquilizing effects [17]. Therefore, it is of great value to develop an efficient and reliable bioanalytical protocol for simultaneous determination of these active ingredients in biological matrix with hopefully maximum response and resolution, and to study the PK properties of them to advance the therapeutic applications of JTT.…”

Er Zhi Wan, an ancient herbal decoction for woman menopausal syndrome, activates the estrogenic response in cultured MCF-7 cells: An evaluation of compatibility in defining the optimized preparation method

“…boiled together in a defined volume of water and herb ratio (Dong et al, 2006); and (ii) Angelica Sinensis Radix has to be processed with 15% alcohol before the making of DBT (Zhan et al, 2011). Thus, the traditional preparation of herbal decoction should be followed unless we have a good reason to change, even though the rationale behind this preparation still has not been fully revealed.…”

“…The classical estrogenic pathway involves the phosphorylation of estrogen receptors and the transcriptional activation of estrogen responsive element (ERE) of target genes. Cultured MCF-7 breast cell is well-known to contain estrogen receptors and to be responsive upon estrogen treatment (Gao et al, 2007;Zhan et al, 2011). In addition, the proliferation of MCF-7 cells could serve as another parameter in revealing the potential risk of developing breast cancer.…”