Chemical analysis and vibrational spectroscopy study of essential oils from Lippia sidoides and of its major constituent

Saraiva, Antônio Gildenberg Queiroz; Saraiva, G.D.; Albuquerque, Roberto Lima de; Nogueira, C.E.S.; Teixeira, Alexandre Magno Rodrigues; Lima, Leandro B.; Cruz, Beatriz Gonçalves; Sousa, Francisco F. de

doi:10.1016/j.vibspec.2020.103111

Cited by 18 publications

(11 citation statements)

References 35 publications

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“…Factors such as harvest time, climate, soil characteristics, geographic origin, temperature, and the presence of flowers influence the biosynthesis of chemotypes and yield of essential oils, which can affect biological activity [ 54 , 55 ]. Thus, the action of essential oils on the enzymes, alkaline phosphatase and acid phosphatase, inhibiting or promoting their production, occurred either by the presence of various chemotypes present in essential oils that acted in synergism or through mechanisms of resistance to plant bioinsecticides.…”

Section: Resultsmentioning

confidence: 99%

Chemical Composition, Larvicidal Activity, and Enzyme Inhibition of the Essential Oil of Lippia grata Schauer from the Caatinga Biome against Dengue Vectors

Felix

Rodrigues

et al. 2021

Pharmaceuticals

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Insect resistance and environmental pollution are among the drawbacks of continuous use of synthetic insecticides against the vectors of dengue, Aedes aegypti and Aedes albopictus. The objective of this study was to analyze the composition of the essential oil of Lippia grata Schauer collected from plants, in three periods of the year, to compare the larvicidal activity and enzymatic inhibition of the dengue vectors. The oilsanalyzed by gas chromatography coupled to mass spectrometry (GC-MS), presented thymol and 1,8-cineole, as the main constituents, in all three periods. This composition was different from that found in previous studies of the species from different places, thus, suggesting a new chemotype of Lippia grata. Larvicidal tests were performed at concentrations of 100, 75, 50, 25, and 12.5 μg.mL−1 and the essential oil from the rainy season showed the best results, with LC50 of 22.79 μg.mL−1 and 35.36 μg.mL−1 against Ae. aegypti and Ae. albopictus, respectively; this result was better than other reports. In the rainy period, however, there was a greater variety of components, which led to a better larvicidal effect, possibly due to synergistic action with minor constituents. Total proteins, amylases, and acetylcholinesterase of both species were inhibited by the oils.

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Section: Resultsmentioning

confidence: 99%

Chemical Composition, Larvicidal Activity, and Enzyme Inhibition of the Essential Oil of Lippia grata Schauer from the Caatinga Biome against Dengue Vectors

Felix

Rodrigues

et al. 2021

Pharmaceuticals

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“…Earlier studies have identified thymol as the main metabolite of EO extracted from L. origanoides from different regions of Brazil [10,[14][15][16][17]19,21,22,[24][25][26][28][29][30][31][32]35,37,38,[40][41][42]45,49,50,[52][53][54][55]57,59,61,[66][67][68][69][70][71][72][73][74][75]. However, some other studies showed that carvacrol was also the main metabolite [8,9,13,27,33,34,36,58,…”

Section: Discussionmentioning

confidence: 99%

“…EOLO is rich in terpenes, mainly carvacrol [8,9,13,27,33,34,36,58,63,64] and thymol [10,[14][15][16][17]19,21,22,[24][25][26][28][29][30][31][32]35,37,38,[40][41][42]45,49,50,[52][53][54][55]57,59,61,[66][67][68][69][70][71][72][73][74][75], responsible for their antibac...…”

Section: Introductionmentioning

confidence: 99%

Extraction, Chemical Composition and in vitro Antibacterial Activity of the Essential Oil of Lippia origanoides Kunth from Northeast Brazil

Silva

Monte

Damaceno

et al. 2022

Braz. arch. biol. technol.

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Lippia origanoides Kunth has among its synonyms Lippia sidoides Cham. and is popularly known as 'alecrim-pimenta' or 'rosemary pepper'. Essential oil of L. origanoides (EOLO) has been previously reported for several biological activities without significant acute toxicity, even at higher doses. In this study, essential oil (EO) was extracted from dried leaves, inflorescences, and branches of L. origanoides from Limoeiro do Norte, Ceará, Brazil by hydrodistillation at 3.63% (w/w) yield. Posteriorly, the presence of chemical compounds in the EOLO was identified by gas chromatography coupled to mass spectrometry (GC-MS). The analysis allowed to identify the thymol as the main metabolite (83.53%), followed by p-cymene (5.92%), caryophyllene (4.31%), γ-terpinene (3.27%), thymol methyl ether (1.18%), β-myrcene (1.00%), and α-terpinene (0.79%). The minimal inhibitory concentration (MIC) of EOLO was assessed against Gramnegative and Gram-positive bacterial pathogens such as Enterobacter aerogenes, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella enterica serovar Typhimurium, and Staphylococcus aureus. At 10 µL/mL concentration, the EOLO exhibited antibacterial activity against all the tested bacterial pathogens, and this concentration was considered as the MIC. The minimum bactericidal concentration (MBC) of EOLO was assessed at the MIC concentration, which inhibited the complete growth of all tested bacterial pathogens after 3 h. Therefore, concentrations less than 10 µL/mL were set as MBC. Thus, our findings suggest that the EOLO can be used as a promising antibacterial agent in the industries. HIGHLIGHTS• Thymol was identified as the main metabolite in the EOLO.• EOLO has antibacterial activity against Gram-negative and Gram-positive bacterial pathogens.• The antibacterial effect of EOLO may be associated with the presence of thymol.

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“…While Morais et al, (2016) working with essential oil of L. origanoides collected from São Gonçalo do Abaeté, in the state of Minas Gerais, Brazil, found MIC of 2,000 μg/mL for samples of P. aeruginosa ATCC 9027 and E. coli ATCC 11229, the plants compound originating from this region had isoborneol (14.66%), bornyl acetate (11.86%), α-humulene (11.23%) as prevalent components. The yield and chemical composition of essential oils are determined by a combination of natural and genomic factors, being therefore variable and these variations will determine physical, chemical, biological and organoleptic properties, defining the usual and commercial purpose of the substance (Saraiva, et al, 2020).…”

Section: Od 620nmmentioning

confidence: 99%

Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistant

Pinheiro

Fontenelle²,

Rodrigues³

et al. 2022

RSD

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The objective of this study was to evaluate the antimicrobial potential of essential oil (EO) from L. origanoides against Gram-negative bacteria with multidrug resistance (MDR) phenotype and to correlate the results obtained with the genetic profile of these microorganisms. The oil was extracted from the leaves of this plant by steam dragging and condensation with Clevenger equipment, the chemical components were identified and quantified by gas chromatography coupled with mass spectrometry and gas chromatography coupled with the flame ionization detector. Twenty clinical isolates and one standard strain of each species were analyzed: E. coli, K. pneumoniae and P. aeruginosa (n=63). The antibacterial potential was evaluated by broth microdilution tests. Thymol is the prevalent component (87.37%). The EO at a concentration of 312 μg/mL was effective in inhibiting 52% of E. coli strains and about 38% of K. pneumoniae. On the other hand, for P. aeruginosa a concentration of 2,500 μg/mL was needed to obtain growth inhibition of approximately 38% of the strains. Among the resistance genes detected, the highest prevalence was blaCTX-M 1/2, however, even with the presence of these genes, an antimicrobial action of this EO was observed. Therefore, the results of this study suggest that the EO of L. origanoides presented an efficient antibacterial action with potential use in the fight against infections by MDR microorganisms.

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Chemical analysis and vibrational spectroscopy study of essential oils from Lippia sidoides and of its major constituent

Cited by 18 publications

References 35 publications

Chemical Composition, Larvicidal Activity, and Enzyme Inhibition of the Essential Oil of Lippia grata Schauer from the Caatinga Biome against Dengue Vectors

Chemical Composition, Larvicidal Activity, and Enzyme Inhibition of the Essential Oil of Lippia grata Schauer from the Caatinga Biome against Dengue Vectors

Extraction, Chemical Composition and in vitro Antibacterial Activity of the Essential Oil of Lippia origanoides Kunth from Northeast Brazil

Inhibitory and bactericidal activities of Lippia origanoides essential oil against Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa multidrug resistant

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